Pharmaceutics,
Год журнала:
2024,
Номер
16(3), С. 312 - 312
Опубликована: Фев. 23, 2024
Curcumin
(CUR)
is
a
natural
compound
that
can
be
combined
with
miconazole
(MCZ)
to
improve
vulvovaginal
candidiasis
(VVC)
caused
by
Candida
albicans
treatment’s
efficacy.
This
study
aimed
develop
ureasil–polyether
(U-PEO)
vaginal
ovules
loaded
CUR
and
MCZ
for
the
treatment
of
VVC.
Physicochemical
characterization
was
performed
thermogravimetry
(TGA),
differential
thermal
analysis
(DTA),
Fourier
transform
infrared
spectroscopy
(FTIR),
in
vitro
release.
Antifungal
assays
were
used
determine
minimum
inhibitory
concentrations
(MICs)
synergism
between
MCZ,
activity
U-PEO
microdilution
agar
diffusion.
TGA
results
showed
high
stability
hybrid
ovules.
In
DTA,
amorphous
character
possible
interaction
observed.
FTIR
no
chemical
incompatibility
drugs.
release
resulted
80%
95%
released
within
144
h.
The
MICs
256
2.5
µg/mL,
respectively.
After
combining
drugs,
MIC
decreased
four-fold
0.625
while
eight-fold
32
µg/mL.
Synergism
confirmed
fractional
concentration
index
(FICI)
equal
0.375.
alone
antifungal
activity.
U-PEO/MCZ
U-PEO/CUR/MCZ
greatest
zones
inhibition
(≥18
mm).
highlight
potential
administered
at
lower
frequency
reduced
doses
compared
available
formulations.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2023,
Номер
13
Опубликована: Май 2, 2023
Antifungal
agents
are
not
always
efficient
in
resolving
vulvovaginal
candidiasis
(VVC),
a
common
genital
infection
caused
by
the
overgrowth
of
Candida
spp.,
including
albicans,
or
preventing
recurrent
infections.
Although
lactobacilli
(which
dominant
microorganisms
constituting
healthy
human
vaginal
microbiota)
important
barriers
against
VVC,
Lactobacillus
metabolite
concentration
needed
to
suppress
VVC
is
unknown.We
quantitatively
evaluated
concentrations
determine
their
effect
on
27
strains
crispatus,
L.
jensenii,
gasseri,
Lacticaseibacillus
rhamnosus,
and
Limosilactobacillus
vaginalis,
with
inhibitory
abilities
biofilms
C.
albicans
clinical
isolates.Lactobacillus
culture
supernatants
suppressed
viable
fungi
approximately
24%-92%
relative
preformed
biofilms;
however,
suppression
differed
among
species.
A
moderate
negative
correlation
was
found
between
lactate
production
biofilm
formation,
but
no
observed
hydrogen
peroxide
formation.
Both
were
required
planktonic
cell
growth.
that
significantly
inhibited
formation
supernatant
also
adhesion
epithelial
cells
an
actual
live
bacterial
competition
test.Healthy
microflora
metabolites
may
play
roles
development
new
antifungal
agent
albicans-induced
VVC.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(15), С. 18434 - 18448
Опубликована: Апрель 5, 2024
The
poor
solubility
of
clotrimazole
in
the
aqueous
medium
and
uncontrolled
removal
drug-loaded
suppository
content
limit
its
effectiveness
treatment
vulvovaginal
candidiasis.
We
present
here
formulations
form
non-Newtonian
structured
fluids,
i.e.,
Bingham
plastic
or
pseudoplastic
fluids
constructed
hyperbranched
polyglycidol,
HbPGL,
with
a
hydrophobized
core
aryl
groups
such
as
phenyl
biphenyl.
amphiphilic
constructs
were
obtained
by
modification
linear
units
containing
monohydroxyl
benzoyl
chloride,
isocyanate,
biphenyl
while
terminal
1,2-diol
shell
protected
during
step,
followed
their
deprotection.
encapsulation
within
internally
HbPGLs
using
solvent
evaporation
method
water
addition
resulted
formation.
Detailed
Fourier-transform
infrared
spectroscopy
(FTIR)
differential
scanning
calorimetry
(DSC)
analyses
performed
for
aryl-HbPGLs
revealed
difference
drug
compatibility
among
polymers.
Clotrimazole
biphenyl-enriched
unlike
derivatives,
was
molecularly
distributed
both
dry
hydrated
states,
resulting
transparent
formulations.
shear-thinning
properties
fluid
make
them
injectable
thus
suitable
intravaginal
application.
Permeability
tests
usage
Franz
diffusion
cell
showed
5-fold
increase
permeability
constant
compared
to
drugs
loaded
commercially
available
disposable
tablet
50-fold
comparison
suspension
clotrimazole.
Furthermore,
biphenyl-modified
HbPGL-based
liquid
enhanced
antifungal
activity
against
Candida
albicans
glabrata
that
retained
up
7
days,
contrast
phenyl-HbPGL
derivatives
tablet.
With
simple
formulation,
convenient
clotrimazole/biphenyl-HbPGL
formulation
strategy,
rheological
properties,
these
systems
are
potential
therapeutics
gynecological
applications.
This
study
points
synthetic
direction
improving
poorly
water-soluble
aryl-enriched
pharmaceuticals.
PLoS ONE,
Год журнала:
2025,
Номер
20(3), С. e0318703 - e0318703
Опубликована: Март 12, 2025
Reptiles
may
act
as
reservoirs
or
spreaders
of
potential
pathogenic
microorganisms
including
Candida
yeasts.
While
the
epidemiology
yeast
species
has
been
thoroughly
studied,
virulence
profile
isolated
is
not
well
investigated.
Therefore,
this
study
aimed
to
assess
haemolytic,
phospholipase,
lipase
activities
and
biofilm
formation
yeasts
from
cloacal
swabs
venomous
snakes
Marrakech,
Morocco
(Group
I,
n
=
40)
non-venomous
Cocullo,
Italy
II,
32).
All
Group
1
showed
low
production
(Lz
≥
0.90)
haemolysin
(Hz
0.90),
only
35%
them
were
phospholipase
(Pz)
producers
(Pz
>
0.90).
In
contrast,
all
2
produced
enzymes
more
than
62%
high
amounts
≤
0.64;
Lz
0.69;
Hz
0.69).
Data
show
that
able
produce
factors,
which
vary
according
hosts
their
origin,
thus
suggesting
role
in
harboring
spreading
environment.
Since
was
lower
ones,
we
discussed
it
be
affected
by
venom
composition.
This
will
pave
way
for
fungal
infection
control,
alternative
antifungal
drugs
order
overcome
resistance
phenomena.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Март 27, 2025
Background
Despite
the
pathogenesis
of
vulvovaginal
candidiasis
(VVC)
is
multifactorial,
this
study
aimed
to
assess
whether
phenotypic
characteristics,
such
as
biofilm
production
and
quality,
along
with
clinical
symptoms,
are
associated
recurrent
VVC
(RVVC).
Methods
Over
1
year
(Oct
2021–Oct
2022),
we
prospectively
included
271
patients
≥18
years
who
attended
our
institution,
had
Candida
spp.
isolated
in
vaginal
swabs,
provided
informed
consent.
Patients
were
followed
for
year.
isolates
tested
by
following
techniques:
crystal
violet
(CV)
biomass
quantification,
XTT
metabolic
activity
microscopy
area
quantification.
Clinical
microbiological
data
also
collected.
Results
Overall,
55
(20.3%)
experienced
at
least
one
recurrence,
19
(7.0%)
meeting
criteria
RVVC
(≥3
episodes/year),
65
episodes
total.
Demographic
characteristics
similar
both
groups.
Most
C.
albicans
(90.0%).
Median
(interquartile,
[IQR])
absorbance
values
CV
18/19
238/252
non-RVVC
follows:
CV,
1.850
(1.578–2.156)
vs.
1.426
(1.081–1.823),
p
=
0.005;
XTT,
0.184
(0.116–0.293)
0.228
(0.147–0.331),
0.253.
(IQR)
occupation
percentage
16/19
16/252
was,
respectively:
13.15
(8.54–16.9)
10.73
(5.88–17.73),
0.710.
Conclusion
was
high
production.
Additionally,
exhibited
a
tendency
toward
lower
activity,
which
may
contribute
treatment
failure.
