Multiplex genome editing for climate-resilient woody plants

Frontiers in Forests and Global Change, Год журнала: 2025, Номер 8

Опубликована: Фев. 5, 2025

Climate change is severely impacting global forest ecosystems, stressing woody plants due to rising temperatures, shifting precipitation patterns, and extreme weather events. These pressures threaten biodiversity disrupt the essential roles forests play in carbon sequestration, timber production, ecosystem stability. Traditional management strategies, such as selective breeding, cannot keep up with rapid pace of climate change, given long juvenile phase trees. Multiplex genome editing, particularly through CRISPR technologies, offers a promising solution accelerate development climate-resilient traits plants. By simultaneously targeting multiple genes, multiplex enables efficient modification polygenic that govern stress tolerance, disease resistance, other crucial resilience factors. This mini-review examines potential technologies management, agroecological practices, showing how they can improve tree support sustainable forestry response growing challenges change.

Язык: Английский

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Genetic Architecture Underlying Response to the Fungal Pathogen Dothistroma septosporum in Lodgepole Pine, Jack Pine, and Their Hybrids

Evolutionary Applications, Год журнала: 2025, Номер 18(2)

Опубликована: Фев. 1, 2025

In recent decades, Dothistroma needle blight (DNB), a pine tree disease caused by the fungal pathogen septosporum, has severely damaged lodgepole (Pinus contorta Dougl. ex. Loud.) in British Columbia, Canada, and raised health concerns for jack banksiana Lamb.). The already shown signs of host shift eastward to hybrid populations between × P. banksiana), possibly into pure pine. However, we have little knowledge about mechanisms resistance D. especially underlying genetic basis variation pines. this study, conducted controlled inoculations induce infection septosporum performed genome-wide case-control association study with pooled sequencing (pool-seq) data dissect architecture response pine, their hybrids. We identified candidate genes associated samples. also assessed structure inferred how introgression may affect distribution involved studied These results can be used develop genomic tools evaluate DNB risk, guide forest management strategies, potentially select resistant genotypes.

Язык: Английский

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The future of rubber production: a review of genetic regulation of biosynthetic pathways and genome editing technologies in natural rubber-producing plants

Tree Genetics & Genomes, Год журнала: 2025, Номер 21(2)

Опубликована: Фев. 13, 2025

Язык: Английский

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Increased genome editing efficiency in poplar by optimizing sgRNA length and copy number

Industrial Crops and Products, Год журнала: 2025, Номер 226, С. 120664 - 120664

Опубликована: Фев. 12, 2025

Язык: Английский

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Application of CRISPR-Cas9 in Agriculture and Forestry

CABI eBooks, Год журнала: 2025, Номер unknown, С. 131 - 145

Опубликована: Март 21, 2025

Язык: Английский

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Advances in Dill (Anethum graveolens L.) Molecular Breeding Strategies

Опубликована: Янв. 1, 2025

Язык: Английский

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CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(23), С. 16656 - 16656

Опубликована: Ноя. 23, 2023

CRISPR (short for "Clustered Regularly Interspaced Short Palindromic Repeats") is a technology that research scientists use to selectively modify the DNA of living organisms. was adapted in laboratory from naturally occurring genome-editing systems found bacteria. In this work, we reviewed methods used introduce CRISPR/Cas-mediated genome editing into fruit species, as well impacts application activate and knock out target genes different tree including on development, yield, quality, tolerance biotic abiotic stresses. The gene-editing could allow development new generations crops with improved traits by targeting genetic segments or even facilitate introduction elite cultivars without changing other traits. However, currently, scarcity efficient regeneration transformation protocols some fact many those procedures are genotype-dependent, convenience segregating transgenic parts system represent main handicaps limiting potential techniques trees. Finally, latest news legislation regulations about plants modified using CRISPR/Cas has been also discussed.

Язык: Английский

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The Use of Gene Editing Technology to Introduce Targeted Modifications in Woody Plants

Опубликована: Янв. 1, 2024

Genome editing technologies help in precise target genome modifications. Various powerful tools such as transcription activator-like effector nucleases (TALENs), zinc-finger (ZFNs), clustered regularly interspaced short palindromic repeats-associated protein systems (CRISPR/Cas), etc. have been used to modify various genomes efficiently. CRISPR/Cas is the most recent technology gene modifications almost all kingdoms of life, including plants. One best uses this can be observed woody plants these show a complexity their genome, viz., huge size, ploidy nature, and long life span. The species-independent nature helps controlling has for studying growth development, wood properties plants, well responses biotic abiotic stresses. This book chapter highlights different advances alteration genes current challenges encountered during use improvement traits, products, germplasm improvement.

Язык: Английский

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Genome editing in Latin America: research achievements and regulatory evolution

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2024, Номер 159(3)

Опубликована: Ноя. 18, 2024

Язык: Английский

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Enhancing Crop Resilience through CRISPR/Cas9-Mediated Development of Disease-Resistant Cultivars

International Journal of Environment and Climate Change, Год журнала: 2023, Номер 13(10), С. 2773 - 2783

Опубликована: Сен. 9, 2023

A major issue in agriculture is the protection of crops against diseases and pests. Plant breeding has been primarily responsible for growth disease-resistant cultivars. The use gene editing techniques plant essential obtaining desired features. Clustered Regular Interspaced Palindromic Repeats (CRISPER)/Cas9 (CRISPR-related protein) a new advancement technology. It can be utilised defence mechanisms pathogen attack by recognising bacterial immune system destroying invasive genes. Advances through CRISPR/Cas9 integration have helped develop cultivars including hereditary resistance to viral diseases. Future crop generations acquire CRISPR/Cas9-mediated transgene if Cas9/sgRNA isolated F1 generation. separation makes safe breeding. Although proven wonderful tool revolutionize various disease resistant varieties, its effect on many physiological processes remains thoroughly investigated.

Язык: Английский

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