Exploring Optimal Biomarker Sources: A Comparative Analysis of Exosomes and Whole Plasma in Fasting and Non-Fasting Conditions for Liquid Biopsy Applications
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
25(1), С. 371 - 371
Опубликована: Дек. 27, 2023
The
study
of
liquid
biopsy
with
plasma
samples
is
being
conducted
to
identify
biomarkers
for
clinical
use.
Exosomes,
containing
nucleic
acids
and
metabolites,
have
emerged
as
possible
sources
biomarkers.
To
evaluate
the
effectiveness
exosomes
over
plasma,
we
analyzed
small
non-coding
RNAs
(sncRNAs)
metabolites
extracted
from
in
comparison
those
directly
whole
under
both
fasting
non-fasting
conditions.
We
found
that
sncRNA
profiles
were
not
affected
by
either
exosome
or
samples.
Our
results
showed
exosomal
sncRNAs
more
consistent
profiles.
miRNA
contained
high
concentrations
cell-derived
miRNAs
likely
due
hemolysis.
determined
certain
exhibited
noteworthy
concentration
shifts
relation
status,
while
others
did
not.
Here,
propose
(1)
required
a
involves
metabolomic
profiling,
long
are
influenced
status
selected,
(2)
utilization
promotes
robust
findings
samples,
mitigating
impact
batch
effects
derived
These
advance
optimization
methodologies
applications.
Язык: Английский
Advancement of Radiolabeled Exosomes in Brain Disorders
Опубликована: Янв. 1, 2024
A
groundbreaking
nanotechnology
is
promised
by
the
exosome
application
in
brain
disorder
treatments
due
to
its
phenomenal
characteristic
of
being
able
cross
blood-brain
barrier
(BBB),
along
with
greater
biocompatibility,
low
immunogenicity,
and
minimal
side
effects.
Exosomes
are
involved
intracellular
communication
as
they
noted
be
heterogeneous
group
nanoscale
membrane-bound
vesicles,
typically
50–100
nm
diameter,
secreted
both
prokaryotic
eukaryotic
cells.
Their
role
seemed
merely
unimportant
for
years,
until
last
two
decades,
when
their
structure,
role,
contents
were
studied
found
that
this
paracrine
cell-to-cell
achieved
substances
carried
cargos,
including
interleukins
cytokines.
Recent
trend
investigations
displayed
how
exosomes
can
come
handy
pathogenesis,
diagnosis,
treatment
several
disorders,
Alzheimer's
disease,
Parkinson's
ischemic
stroke,
many
more,
natural
ability
load
hydrophilic
hydrophobic
compounds
while
crossing
BBB.
Currently,
RMT
(receptor-mediated
transcytosis)
prominent
method
application,
where
ligands
expressed,
such
LDLR-targeting
apolipoprotein
B,
engineered
demonstrate
much
efficient
drug
uptake
parenchyma.
As
comprehensive
neurological
disorders
currently
progress,
it
crucial
comprehend
nature
potential
these
exosomes;
thus,
radiolabeled
provide
noninvasive
tracking
understanding
activity
action,
imaging
signal
tissue
penetration
accurate
quantification.
per
recent
studies,
higher
sensitivity,
specificity,
versatility
noninvasive.
This
chapter
will
explore
nano-drug
delivery
system,
isolation
innovative
means,
physiochemical
properties
focus
on
latest
progression
technology
underlying
BBB
ability,
summarizing
natural,
engineered,
assisted
brain-targeted
exosome-oriented
treatment.
We
expect
also
make
our
readers
major
challenges
terms
kinetics
clinical
applications.
Язык: Английский
Proteomic Dissection of Exosome Cargo: Progress and Future Perspectives
Cancers,
Год журнала:
2023,
Номер
15(17), С. 4292 - 4292
Опубликована: Авг. 28, 2023
The
interest
in
exosomes
cancer
research
and
treatment
has
increased
exponentially
the
past
few
years
[...].
Язык: Английский