MicroRNAs in Plasma-Derived Extracellular Vesicles as Non-Invasive Biomarkers for Eosinophilic Esophagitis

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 639 - 639

Опубликована: Янв. 14, 2025

Eosinophilic esophagitis (EoE) is a chronic inflammatory esophageal disorder. The lack of non-invasive biomarkers currently results in dependency on endoscopy with biopsies for its diagnosis and monitoring. We aimed to identify potential using microRNAs (miRNAs) plasma-derived extracellular vesicles (pEVs). This was prospective single-center observational study discovery cohort EoE patients (n = 26) active disease (EoE.Basal) after anti-inflammatory treatment (EoE.Post.tx) control subjects 16). Small-RNA-seq performed differentially regulated small RNAs (sRNAs). Candidate miRNAs were validated an independent (EoE patients, n 33; controls, 14). pEVs-sRNA cargo differed among conditions. Compared Ser_Comb_22, Leu_Comb_5, miR-10b-5p, miR-125a-5p upregulated EoE.Basal, miR-224-5p, miR-221-3p, let-7d-5p, miR-191-5p downregulated. combination miR-221-3p miR-10b-5p showed the best diagnostic performance. Comparing paired samples, miR-374a-5p miR-30a-3p while miR-15a-5p let-7d-5p Combined AUC values, alone as monitoring biomarker (p 0.001). In conclusion, changed upon inflammation proposed treatment. first explore use pEVs EoE.

Язык: Английский

---

Proteomic analysis of plasma-derived extracellular vesicles: pre- and postprandial comparisons

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 3, 2024

Язык: Английский

---

Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer

Cell Death and Disease, Год журнала: 2024, Номер 15(10)

Опубликована: Окт. 16, 2024

Radiotherapy represents a major curative treatment for prostate cancer (PCa), but some patients will develop radioresistance (RR) and relapse. The underlying mechanisms remain poorly understood, miRNAs might be key players in the acquisition maintenance of RR. Through their encapsulation small extracellular vesicles (EVs), they can also relevant biomarkers radiation response. Using next-generation sequencing, we found that miR-200c-3p was downregulated PCa RR cells EVs due to gain methylation on its promoter during acquisition. We next showed exogenous overexpression restores radiosensitivity by delaying DNA repair through targeting HP1α. Interestingly, observed downregulation expression radiation-resistant lung breast cell lines. In summary, our study demonstrates could help distinguish radioresistant from sensitive tumor cells. This miRNA targets HP1α delay promote death.

Язык: Английский

---

Recommended Clinical Context and Patient Context Data Elements for Liquid Biopsy Data Submitted to Data Repositories and Data Commons

Clinical and Translational Science, Год журнала: 2025, Номер 18(4)

Опубликована: Апрель 1, 2025

ABSTRACT In 2020, BLOODPAC recommended 11 pre‐analytical minimal technical data elements for collection and submission of liquid biopsy to public databases. This article expands on that work by recommending 22 clinical context 10 patient elements. These elements, essential submitted repositories like the Data Commons, cover tumor characteristics, disease progression, demographics, supporting biomarker validation, research, trials.

Язык: Английский

---

Altered exosomal miRNA profiles in patients with paraneoplastic cerebellar degeneration

Annals of Clinical and Translational Neurology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 29, 2024

Abstract Objective Patients with ovarian cancer (OC) may develop anti‐Yo‐associated paraneoplastic cerebellar degeneration (PCD)—a ataxia associated tumor‐induced autoimmunity against CDR2 and CDR2L proteins. Dysregulation of circulating exosomal microRNAs (miRNAs) occur in OC. Here, we investigated whether PCD is changes the miRNA profiles OC patients. Methods Serum exosomes were isolated from patients ( n = 15 ), 14 ) healthy controls (HC, ). Small RNA sequencing was used to identify differentially expressed miRNAs. Receiver operating characteristic curves evaluate biomarker sensitivity specificity, target prediction analysis employed elucidate gene targets. Results exhibited a distinct expression profile. We detected 103 miRNAs compared without 139 controls. Particularly miR‐486‐5p, miR‐4732‐5p, miR‐98‐5p miR‐21‐5p notable specificity for discriminating both showed that several targeted genes. Interpretation Our results demonstrate exhibit profile PCD. Several diagnostic potential hold relevance understanding pathogenesis

Язык: Английский

---

Circulating pre- and postprandial extracellular vesicle proteomic profiles

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 4, 2024

Extracellular vesicles (EVs) are key in intercellular communication, carrying biomolecules like nucleic acids, lipids, and proteins. This study investigated postprandial characteristics proteomic profiles of circulating large extracellular (lEVs) healthy individuals. Twelve participants fasted overnight before baseline assessments. After consuming a controlled isocaloric meal, lEVs were isolated for flow cytometric analysis. Plasma triacylglyceride (TAG) levels confirmed fasting completion, while protein concentrations plasma monitored stability. Proteomic analysis identified upregulated proteins related to transport mechanisms epithelial/endothelial functions postprandially, indicating potential roles physiological responses nutritional intake. Enrichment analyses revealed vesicle-related pathways immune system processes. Flow cytometry showed increased expression CD324 on medium-sized CD9⁺CD63⁺CD81⁺ EVs suggesting an epithelial origin. These findings offer insights into lEV dynamics their significance, highlighting the need stringent guidelines EV studies account effects composition function.

Язык: Английский

---