Acta Tropica, Год журнала: 2024, Номер 254, С. 107182 - 107182
Опубликована: Март 11, 2024
Язык: Английский
Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)
Опубликована: Март 11, 2023
The advancement of microfluidics has enabled numerous discoveries and technologies in life sciences. However, due to the lack industry standards configurability, design fabrication microfluidic devices require highly skilled technicians. diversity discourages biologists chemists from applying this technique their laboratories. Modular microfluidics, which integrates standardized modules into a whole, complex platform, brings capability configurability conventional microfluidics. exciting features, including portability, on-site deployability, high customization motivate us review state-of-the-art modular discuss future perspectives. In review, we first introduce working mechanisms basic evaluate feasibility as components. Next, explain connection approaches among these modules, summarize advantages over integrated biological applications. Finally, challenge perspectives
Язык: Английский
Процитировано
48
Regenerative Biomaterials, Год журнала: 2024, Номер 11
Опубликована: Янв. 1, 2024
Abstract Biofabrication techniques allow for the construction of biocompatible and biofunctional structures composed from biomaterials, cells biomolecules. Bioprinting is an emerging 3D printing method which utilizes biomaterial-based mixtures with other biological constituents into printable suspensions known as bioinks. Coupled automated design protocols based on different modes droplet deposition, bioprinters are able to fabricate hydrogel-based objects specific architecture geometrical properties, providing necessary environment that promotes cell growth directs differentiation towards application-related lineages. For preparation such bioinks, various water-soluble biomaterials have been employed, including natural synthetic biopolymers, inorganic materials. Bioprinted constructs considered be one most promising avenues in regenerative medicine due their native organ biomimicry. a successful application, bioprinted should meet particular criteria optimal response, mechanical properties similar target tissue, high levels reproducibility fidelity, but also increased upscaling capability. In this review, we highlight recent advances bioprinting, focusing regeneration tissues bone, cartilage, cardiovascular, neural, skin organs liver, kidney, pancreas lungs. We discuss rapidly developing co-culture bioprinting systems used resemble complexity crosstalk between populations regeneration. Moreover, report basic physical principles governing ideal bioink biomaterials’ potential. examine critically present status regarding its applicability current limitations need overcome establish it at forefront artificial production transplantation.
Язык: Английский
Процитировано
12
Biosensors, Год журнала: 2024, Номер 14(7), С. 336 - 336
Опубликована: Июль 10, 2024
Cell co-culture technology aims to study the communication mechanism between cells and better reveal interactions regulatory mechanisms involved in processes such as cell growth, differentiation, apoptosis, other cellular activities. This is achieved by simulating complex organismic environment. Such studies are of great significance for understanding physiological pathological multicellular organisms. As an emerging cultivation technology, 3D based on microfluidic chips, can efficiently, rapidly, accurately achieve co-culture. accomplished leveraging unique microchannel structures flow characteristics chips. The simulate native microenvironment providing a new technical platform studying intercellular communication. It has been widely used research oncology, immunology, neuroscience, fields. In this review, we summarize provide insights into design systems detection methods employed systems, applications these models.
Язык: Английский
Процитировано
8
Cyborg and Bionic Systems, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
Three-dimensional (3D) neural cultures are increasingly recognized for their complexity and resemblance to in vivo microenvironments. In this paper, we present a novel 3D cell culturing noninvasive characterization technique of spheroids. Based on embedded platinum wires, the cultured cells lollipop-shaped spheroids where axons extended integrated around wires. Electrical microstimulation enhanced connectivity between demonstrated signal propagation among them. The resultant axonal elongation facilitated formation robust tracts interconnecting Variation cells' density allows adjust spheroid's diameter, identifying 1 million as good number spheroid formation. Recordings activities reveal higher-quality measurement from interior compared those obtained exterior cells. Viability assays confirmed efficacy proposed sustained growth over 1-month period. holds potential applications various fields, such development brain organoids, which enables real-time interconnection sensing environment conditions.
Язык: Английский
Процитировано
1
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 9, 2025
Язык: Английский
Процитировано
1
Journal of NeuroVirology, Год журнала: 2023, Номер 29(2), С. 121 - 134
Опубликована: Апрель 1, 2023
Язык: Английский
Процитировано
19
Clinical and Translational Science, Год журнала: 2023, Номер 17(2)
Опубликована: Дек. 8, 2023
In vitro and in vivo models play integral roles preclinical drug research, evaluation, precision medicine. primarily involve research platforms based on cultured cells, typically the form of two-dimensional (2D) cell models. However, notable disparities exist between 2D cells across various aspects, rendering former inadequate for replicating physiologically relevant functions human or animal organs tissues. Consequently, these failed to accurately reflect real-life scenarios post-drug administration. Complex (CIVMs) refer that integrate a multicellular environment three-dimensional (3D) structure using bio-polymer tissue-derived matrices. These seek reconstruct organ- tissue-specific characteristics extracellular microenvironment. The utilization CIVMs allows enhanced physiological correlation thereby better mimicking conditions without ethical concerns associated with experimentation. have gained prominence disease development. This review aimed comprehensively examine analyze types, manufacturing techniques, applications CIVM domains discovery, development, objective this study was provide comprehensive understanding progress made their potential future use fields.
Язык: Английский
Процитировано
19
Clinical and Translational Medicine, Год журнала: 2024, Номер 14(4)
Опубликована: Апрель 1, 2024
Abstract Background Organoid technology is an emerging and rapidly growing field that shows promise in studying organ development screening therapeutic regimens. Although organoids have been proposed for a decade, concerns exist, including batch‐to‐batch variations, lack of the native microenvironment clinical applicability. Main body The concept has derived patient‐derived tumour (PDTOs) personalized drug new discovery, mitigating risks medication misuse. greater similarity between PDTOs primary tumours, more influential model will be. Recently, ‘tumour assembloids’ inspired by cell‐coculture attracted attention to complement current PDTO technology. High‐quality must reassemble critical components, multiple cell types, matrix, paracrine factors, angiogenesis microorganisms. This review begins with brief overview history PDTOs, followed approaches generating assembloids. Personalized practised; however, it remains unclear whether can predict immunotherapies, immune drugs (e.g. checkpoint inhibitors) cells tumour‐infiltrating lymphocyte, T receptor‐engineered chimeric antigen receptor‐T cell). as cancer avatars patients, be expanded stored form biobank. Conclusion Fundamental research trials are ongoing, intention use these models replace animals. Pre‐clinical immunotherapy using beneficial patients. Key Points not yet constructed key cellular non‐cellular components. should expandable editable. promising preclinical unless mature established. biobanks consensual standards urgently needed.
Язык: Английский
Процитировано
8
Interdisciplinary materials, Год журнала: 2023, Номер 2(5), С. 714 - 734
Опубликована: Сен. 1, 2023
Abstract The three‐dimensional (3D) multicellular platforms prepared by cells or biomaterials have been widely applied in biomedical fields for the regeneration of complex tissues, exploration cell crosstalk, and establishment tissue physiological pathological models. Compared with traditional 2D culture methods, 3D are easier to adjust components structures extracellular matrix (ECM) because synthesis ECM use biomaterials. Moreover, also can customize distribution precisely design micro macro systems. Based on these typical advantages their increasingly important position field, this review summarizes present platforms. Herein, current divided into two major types: scaffold‐free scaffold‐based specific characteristics applications different types thoroughly introduced help readers understand how models affect regulate behaviors inspire researchers select suitable according application scenarios.
Язык: Английский
Процитировано
14
Exploration of Targeted Anti-tumor Therapy, Год журнала: 2024, Номер 5(2), С. 409 - 431
Опубликована: Апрель 25, 2024
Colorectal cancer (CRC) is a heterogeneous disease. Conventional two-dimensional (2D) culture employing cell lines was developed to study the molecular properties of CRC in vitro. Although these which are isolated from tumor niche develop, translation human model such as studying drug response often hindered by inability recapture original features and lack clinical tumors represented this 2D model, differed vivo condition. These limitations may be overcome utilizing three-dimensional (3D) consisting spheroids organoids. Over past decade, great advancements have been made optimizing method establish organoids solid including for multiple purposes screening establishing personalized medicine. structures proven versatile robust models progression deciphering its heterogeneity. This review will describe on advances 3D technology application well challenges CRC-derived mode screen anticancer drugs.
Язык: Английский
Процитировано
4