Archives of Medical Science,
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 25, 2023
Introduction
Hyperbaric
oxygen
(HBO2)
therapy
involves
the
inhalation
of
pure
in
a
pressure
chamber
under
increased
ambient
pressure.
Recent
research
indicates
that
circulating
small
extracellular
vesicles
(sEVs)
play
important
roles
human
physiology
and
pathology.
Therefore,
objective
this
pilot
study
was
to
monitor
impact
HBO2
on
levels
sEVs
serum
patients
with
necrotizing
soft-tissue
infections
(NSTI),
aseptic
bone
necrosis
(ABN)
or
idiopathic
sudden
sensory
neural
hearing
loss
(ISSNHL).
Material
methods
Serum-derived
were
isolated
quantified
80
before
after
applied
for
NSTI,
ISSNHL
ABN
as
well
normal
controls
who
received
neither
nor
steroids.
Results
We
observed
significant
increase
(p
<
0.05),
significantly
elevated
compared
NSTI
0.05)
0.01).
Conclusions
The
may
be
evidence
both
intended
reduction
inflammation
result
steroid
inhibitory
effect
oxidative
stress
induced
by
therapy.
Thus,
released
during
might
an
biological
role
mediating
response
promising
approach
gain
further
insights
into
therapeutic
efficacy
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 28, 2024
Malignancy
is
a
major
public
health
problem
and
among
the
leading
lethal
diseases
worldwide.
Although
current
tumor
treatment
methods
have
therapeutic
effect
to
certain
extent,
they
still
some
shortcomings
such
as
poor
water
solubility,
short
half-life,
local
systemic
toxicity.
Therefore,
how
deliver
agent
so
realize
safe
effective
anti-tumor
therapy
become
urgently
be
solved
in
this
field.
As
medium
of
information
exchange
material
transport
between
cells,
exosomes
are
considered
promising
drug
delivery
carrier
due
their
nano-size,
good
biocompatibility,
natural
targeting,
easy
modification.
In
review,
we
summarize
recent
advances
isolation,
identification,
loading,
modification
carriers
for
alongside
application
therapy.
Basic
knowledge
exosomes,
biogenesis,
sources,
characterization
methods,
also
introduced
herein.
addition,
challenges
related
use
vehicles
discussed,
along
with
future
trends.
This
review
provides
scientific
basis
exosome
systems
oncological
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
Biomedicines,
Год журнала:
2025,
Номер
13(2), С. 275 - 275
Опубликована: Янв. 23, 2025
Background:
Traumatic
brain
injury
(TBI)
occurs
in
individuals
of
all
ages,
predominantly
during
sports,
accidents,
and
active
military
service
members.
Chronic
consequences
TBI
include
declined
cognitive
motor
function,
dementia,
emotional
distress.
Small
extracellular
vesicles
(sEVs),
previously
referred
to
as
exosomes,
are
nano-sized
lipid
that
play
a
role
intercellular
communication.
Our
prior
research
established
the
efficacy
sEVs
derived
from
human
adipose
stem
cells
(hASC
sEVs)
accelerating
healing
injuries.
The
hASC
biologic
therapeutic
need
be
stored
at
−20
°C
or
−80
°C.
This
limits
their
use
translating
everyday
clinics
inclusion
first-aid
kits
for
application
immediately
after
injury.
To
address
this,
here
we
demonstrate
can
room
temperature
(RT)
two
months
post
lyophilization.
Methods:
A
transmission
electron
microscope
(TEM)
nanoparticle
tracking
analysis
(NTA)
were
used
validate
morphology
lyophilized
RT
sEVs.
Using
vitro
models
neuronal
mimicking
physical
injury,
inflammation,
oxidative
stress,
viable
promote
Results:
maintain
purity,
size,
upon
rehydration.
Lyophilized,
showed
better
compared
with
Conclusions:
storage
maintains
accelerate
injuries
cells.
will
advance
sEVs,
bringing
them
closer
clinics,
home
kits,
on
battlefields
by
Nanomedicine,
Год журнала:
2024,
Номер
19(10), С. 915 - 941
Опубликована: Март 6, 2024
Chronic
nonhealing
wounds
pose
a
serious
challenge
to
regaining
skin
function
and
integrity.
Platelet-derived
extracellular
vesicles
(PEVs)
are
nanostructured
particles
with
the
potential
promote
wound
healing
since
they
can
enhance
neovascularization
cell
migration
reduce
inflammation
scarring.
This
work
provides
an
innovative
overview
of
technical
laboratory
issues
in
PEV
production,
PEVs'
role
chronic
benefits
challenges
its
clinical
translation.
The
article
also
explores
proper
sourcing,
extraction
techniques
storage
conditions,
discusses
necessity
further
evaluations
combinational
therapeutics,
including
dressing
biomaterials,
M2-derived
exosomes,
mesenchymal
stem
cells-derived
microneedle
technology,
boost
their
therapeutic
efficacy
as
advanced
strategies
for
healing.
Pharmaceutics,
Год журнала:
2024,
Номер
16(11), С. 1394 - 1394
Опубликована: Окт. 29, 2024
In
the
tenth
year
since
first
edition
of
MISEV
was
released
in
2014,
MISEV2023
has
been
reported
2024
with
aim
refining
standard
and
improving
rigor,
reproducibility,
transparency
extracellular
vesicle
(EV)
research
to
clarify
requirements
for
experimental
design
EVs,
emphasize
importance
reproducible
results
as
well
encouraging
openness
information.
The
release
significantly
contributed
quality
field
which
creates
a
more
reliable
environment.
However,
despite
important
role
MISEV,
there
is
still
need
EV
researchers
continue
push
widespread
implementation
guidelines
meet
evolving
nature
challenges
research.
evolution
attention
it
receives
have
grown
exponentially
over
time,
number
people
involved
writing
MISEV.
Here,
this
review
briefly
summarizes
three
editions
aiming
recall
MISEV2014
MISEV2018
while
learning
about
latest
release,
MISEV2023,
gain
deeper
understanding
content,
provide
quick
note
beginners
who
want
learn
explore
world.
ACS Omega,
Год журнала:
2023,
Номер
8(50), С. 47380 - 47392
Опубликована: Дек. 7, 2023
Extracellular
vesicles
(EVs)
are
membranous
structures
secreted
by
various
cells
carrying
diverse
biomolecules.
Recent
advancements
in
EV
glycosylation
research
have
underscored
their
crucial
role
cancer.
This
review
provides
a
global
overview
of
research,
covering
aspects
such
as
specialized
techniques
for
isolating
and
characterizing
glycosylation,
advances
on
how
affects
the
biogenesis
uptake
EVs,
involvement
intracellular
protein
expression,
cellular
metastasis,
intercellular
interactions,
potential
applications
immunotherapy.
Furthermore,
through
an
extensive
literature
review,
we
explore
recent
context
cancer,
with
focus
lung,
colorectal,
liver,
pancreatic,
breast,
ovarian,
prostate,
melanoma
cancers.
The
primary
objective
this
is
to
provide
comprehensive
update
researchers,
whether
they
seasoned
experts
field
EVs
or
newcomers,
aiding
them
exploring
new
avenues
gaining
deeper
understanding
mechanisms.
heightened
comprehension
not
only
enhances
researchers'
knowledge
pathogenic
mechanisms
but
also
paves
way
innovative
cancer
diagnostic
therapeutic
strategies.
Journal of Autoimmunity,
Год журнала:
2024,
Номер
147, С. 103255 - 103255
Опубликована: Май 25, 2024
To
investigate
the
epigenetic
footprint
of
idiopathic
inflammatory
myopathies
(IIM)
through
characterization
circulating
extracellular
vesicles
(EVs)
and
expression
EV-derived
small
non-coding
RNAs
(sncRNAs).
In
this
cross-sectional
study,
EVs
were
isolated
by
size-exclusion
chromatography
from
plasma
patients
with
IIM
age-
sex-matched
healthy
donors
(HD).
sncRNAs
sequenced
quantified
using
Next-Generation
Sequencing
(NGS).
Following
quality
control
normalization,
filtered
count
reads
used
for
differential
microRNA
(miRNA)
piwi-interacting
RNA
(piRNA)
analyses.
Putative
gene
targets
enriched
pathways
implicated
in
analyzed.
Patients'
clinical
laboratory
characteristics
at
time
sampling
recorded.
Forty-seven
45
HD
enrolled.
MiR-486-5p
(p
<
0.01),
miR-122-5p,
miR-192-5p,
miR-32-5p
significantly
upregulated
0.05
all),
while
miR-142-3p
0.001),
miR-141-3p
let-7a-5p
0.05)
miR-3613-5p
downregulated
versus
HD.
was
associated
raised
muscle
enzymes
levels.
Several
target
genes
up/downregulated
miRNAs
participate
inflammation,
necroptosis,
interferon
immune
signaling.
Six
piRNAs
dysregulated
0.05).
Within
IIM,
miR-335-5p
selectively
miR-27a-5p
dermatomyositis
(n
=
21,
p
0.01).
Finally,
EV
levels
increased
cancer-associated
myositis
(CAM,
n
12)
non-CAM
35,
0.02)
cargo
CAM
let-7f-5p
depleted
miR-143-3p.
Through
an
unbiased
screening
sncRNAs,
we
characterize
as
potential
biomarkers
modifiers
diverse
phenotypes.
