The Role of Fibroblasts in Skin Homeostasis and Repair

Biomedicines, Год журнала: 2024, Номер 12(7), С. 1586 - 1586

Опубликована: Июль 17, 2024

Fibroblasts are typical mesenchymal cells widely distributed throughout the human body where they (1) synthesise and maintain extracellular matrix, ensuring structural role of soft connective tissues; (2) secrete cytokines growth factors; (3) communicate with each other cell types, acting as signalling source for stem niches; (4) involved in tissue remodelling, wound healing, fibrosis, cancer. This review focuses on developmental heterogeneity dermal fibroblasts, their ability to sense changes biomechanical properties surrounding aging, skin repair, pathologic conditions tumour development. Moreover, we describe use fibroblasts different models (e.g., vivo animal vitro systems from 2D 6D cultures) bioengineering informative potential high-throughput assays study under disease contexts personalized healthcare regenerative medicine applications.

Язык: Английский

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Research Progress in Skin Aging and Immunity

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(7), С. 4101 - 4101

Опубликована: Апрель 7, 2024

Skin aging is a complex process involving structural and functional changes characterized by decrease in collagen content, reduced skin thickness, dryness, the formation of wrinkles. This underpinned multiple mechanisms including free radical theory, inflammation photoaging metabolic theory. The immune system, an indispensable part body’s defense mechanism, comprises macrophages, lymphocytes, dendritic cells, mast cells. These cells play pivotal role maintaining homeostasis responding to injury or infection. As age advances, along with various internal external environmental stimuli, may undergo senescence accelerated aging, cell division capability, increased mortality, gene expression patterns signaling pathways, altered functions. collectively impact overall function system. review summarizes relationship between immunity explores characteristics composition effects these on aging. Immune dysfunction plays significant suggesting that immunoregulation become one important strategies for prevention treatment

Язык: Английский

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Clinically Actionable Topical Strategies for Addressing the Hallmarks of Skin Aging: A Primer for Aesthetic Medicine Practitioners

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Янв. 19, 2024

In this narrative review, we sought to provide a comprehensive overview of the mechanisms underlying cutaneous senescence, framed by twelve traditional hallmarks aging. These include genomic instability, telomere attrition, epigenetic alterations, loss proteostasis, impaired macroautophagy, deregulated nutrient sensing, mitochondrial dysfunction, cellular stem cell exhaustion, altered intercellular communication, chronic inflammation, and dysbiosis. We also examined how topical interventions targeting these can be integrated with conventional aesthetic medicine techniques enhance skin rejuvenation. The potential combining targeted therapies against aging minimally invasive procedures represents significant advancement in medicine, offering personalized effective strategies combat reviewed evidence paves way for future advancements underscores transformative integrating scientifically validated into practices.

Язык: Английский

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A highly biocompatible CE-crosslinked collagen implant with exceptional anti-calcification and collagen regeneration capabilities for aging skin rejuvenation

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(18), С. 4467 - 4477

Опубликована: Янв. 1, 2024

Skin aging, a complex and inevitable biological process, results in wrinkles, dermal laxity, skin cancer, profoundly influencing appearance overall health.

Язык: Английский

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Senomorphic activity of a combination of niacinamide and hyaluronic acid: correlation with clinical improvement of skin aging

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 15, 2024

Intrinsic and extrinsic factors, including lifestyle sun exposure, can contribute to cell senescence, which impairs skin homeostasis, that may in turn lead aging. Senescent cells have a specific secretome, called the senescence-associated secretory phenotype (SASP) includes MMPs, CXCLs S100A8/9. Reducing SASP with senotherapeutics is promising strategy reduce Here we evaluated effect of formula containing niacinamide hyaluronic acid, are known limit senescence We conducted three different studies. (1) Ex vivo explants treated had more collagen glycosaminoglycan. (2) In clinical trial forty-four women, two months treatment improved fine lines, wrinkles, luminosity, smoothness, homogeneity, plumpness. (3) third study on thirty one arm for took biopsies gene expression. 101 mRNAs 13 miRNAs were differentially expressed. observed likely senomorphic effect, as there was decrease many genes MMP12 CXCL9 significant downregulation autocrine signaling genes: S100A8 S100A9. These pharmaco-clinical results first demonstrate properties an effective anti-aging skin.

Язык: Английский

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Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract

Antioxidants, Год журнала: 2024, Номер 13(1), С. 105 - 105

Опубликована: Янв. 15, 2024

Photoaging is a process related to an increased level of reactive oxygen species (ROS). Polyphenols can scavenge free radicals in the body, which delay skin aging. Therefore, our work aimed prepare biologically active extract from dry fruits Vaccinium myrtillus or corymbosum and use it for preparation hydrogels topical application. eight different extracts (using V. extraction mixtures: methanol, methanol–water 1:1, water, acetone–water 1:1) were prepared their phytochemical (total polyphenolic content, total flavonoid anthocyanin content) biological properties (antioxidant, anti-hyaluronidase, anti-tyrosinase activity) assessed. Cytotoxicity towards HaCaT keratinocytes was also determined. Based on results, selected further study. Using Design Experiments approach, chitosan-based with bilberry fruit prepared. The content chitosan as independent factors. activity depended content; however, enzyme-inhibiting (anti-hyaluronidase anti-tyrosinase) resulted presence both chitosan. Increased concentration hydrogel base led viscosity and, consequently, slower release compounds. To get optimal characteristics, 1% 2.5% MMW utilized. research suggests validity using preparations anti-aging properties.

Язык: Английский

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ROS Scavenging Effect of Selected Isoflavones in Provoked Oxidative Stress Conditions in Human Skin Fibroblasts and Keratinocytes

Molecules, Год журнала: 2024, Номер 29(5), С. 955 - 955

Опубликована: Фев. 22, 2024

Isoflavones, belonging to polyphenolic compounds, show structural similarity natural estrogens, and in this context, they have been extensively studied. Some of them are also applied as cosmetic additives; however, little is known regarding their effects on skin cells. In investigation, common isoflavones, including genistein, daidzein, glycitein, formononetin, biochanin A, well coumestrol, were evaluated for antioxidant activity impact human fibroblasts keratinocytes. Antioxidant assessed using DPPH, ABTS, FRAP tests, the ability scavenge reactive oxygen species (ROS) was tested cells with H2O2-provoked oxidative stress. The enzymes (SOD, CAT, GSH) lipid peroxidation (MDA) explored. As shown by Alamar Blue neutral red uptake assays, compounds not toxic within concentration range, formononetin coumestrol even demonstrated a stimulatory effect Coumestrol A significant antioxidative potential, leading decrease ROS stimulated H2O2. Furthermore, influenced enzyme activity, preventing depletion during induced stress, reduced MDA levels, demonstrating protection against peroxidation. turn, glycitein exhibited low capacity.

Язык: Английский

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Human Probiotic Lactobacillus paracasei-Derived Extracellular Vesicles Improve Tumor Necrosis Factor-α-Induced Inflammatory Phenotypes in Human Skin

Cells, Год журнала: 2023, Номер 12(24), С. 2789 - 2789

Опубликована: Дек. 7, 2023

Lactic acid bacteria (LAB), a probiotic, provide various health benefits. We recently isolated new Lactobacillus paracasei strain with strong anti-inflammatory effects under lipopolysaccharide-induced conditions and proposed mode of action—augmenting the endoplasmic reticulum stress pathway for functions in host cells. The beneficial L. strains on skin have been described; however, paracasei-derived extracellular vesicles (LpEVs) are poorly understood. Herein, we investigated whether LpEVs can improve inflammation-mediated phenotypes by determining their primary human cells three-dimensional (3D) full-thickness equivalent tumor necrosis factor (TNF)-α-challenged inflammatory conditions. were efficiently taken up much less cytotoxic to than bacterial lysates. Furthermore, low LpEV concentrations restored TNF-α-induced cellular phenotypes, resulting increased cell proliferation collagen synthesis, but decreased levels (matrix metalloproteinase 1, interleukin 6, 8) dermal fibroblasts, which was comparable that retinoic acid, representative antiaging compound. validated 3D model. treatment remarkably epidermal malformation, abnormal keratinocytes basal layer, reduction synthesis. Additionally, penetrated reached deepest layer within 24 h when overlaid top equivalent. they possessed superior antioxidant capacity compared cell-derived EVs. Taken together, probiotic be attractive substances improving inflammation-induced disorders.

Язык: Английский

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Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of Lactobacillus iners Derived from Korean Women’s Skin in Their Twenties

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(1), С. 513 - 526

Опубликована: Янв. 4, 2024

The process of skin aging is intricate, involving intrinsic aging, influenced by internal factors, and extrinsic mainly caused exposure to UV radiation, resulting in photoaging. Photoaging manifests as issues such wrinkles discoloration. microbiome, a diverse community microorganisms on the skin’s surface, plays crucial role protection can be affected factors like humidity pH. Probiotics, beneficial microorganisms, have been investigated for their potential enhance health regulating microbiome. This accomplished through oral probiotics, impacting gut–skin axis, or topical applications introducing live bacteria skin. Probiotics mitigate oxidative stress, suppress inflammation, maintain extracellular matrix, ultimately averting aging. However, research probiotics derived from human limited, there no established product preventing mechanism which shield microbiome layers radiation remains unclear. Recently, researchers discovered Lactobacillus skin, with reports indicating decrease this microorganism age. In recent study, scientists isolated iners KOLBM20 individuals twenties confirmed its effectiveness. A comparative analysis genetic sequences revealed that strain belongs genus closely relates L. DSM13335(T) 99.20% similarity. Importantly, displayed anti-wrinkle properties inhibiting MMP-1. investigation demonstrated inhibitory effect lysate MMP-1 expression. Moreover, data suggest may prevent UVB-induced expression activation ERK, JNK, p38 signaling pathways induced UVB. Consequently, holds promise therapeutic agent treating

Язык: Английский

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Exploring Anti-Aging Effects of Topical Treatments for Actinic Keratosis

Medicina, Год журнала: 2025, Номер 61(2), С. 207 - 207

Опубликована: Янв. 24, 2025

Background and Objectives: Actinic keratosis (AK) is a precancerous cutaneous lesion driven by chronic ultraviolet (UV) exposure, often coexisting with features of photoaging, such as wrinkles pigmentary irregularities. Recent evidence suggests that treatments for AK may also counteract photoaging through shared molecular pathways, including oxidative stress inflammation. This narrative review explores the dual benefits therapies, highlighting their potential anti-aging skin-lightening effects, implications improving skin appearance alongside clearance. Materials Methods: The literature was analyzed to assess efficacy, mechanisms, cosmetic outcomes commonly used treatments, topical agents (5-fluorouracil (5-FU), imiquimod, diclofenac, tirbanibulin), photodynamic therapy (PDT). Studies effects on photoaged skin, collagen remodeling, pigmentation, patient satisfaction were reviewed. Results: PDT emerged most validated treatment, demonstrating improved synthesis, texture, pigmentation. 5-FU showed remodeling dermal matrix increased procollagen levels, but local reactions represent major limitation. Imiquimod enhanced fibroplasia reduced solar elastosis, while diclofenac provided mild photodamage improvements minimal adverse effects. Tirbanibulin promising aesthetic outcomes, lightening reduction in mottled favorable tolerability. Conclusions: therapies offer dual-purpose strategy, addressing both lesions concerns associated photoaging. While remains gold standard, emerging like tirbanibulin ointment exhibit substantial potential. Future research should focus optimizing treatment protocols evaluating long-term enhance compliance.

Язык: Английский

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