Integrative bioinformatics analysis to identify ferroptosis-related genes in non-obstructive azoospermia

Journal of Assisted Reproduction and Genetics, Год журнала: 2024, Номер 41(8), С. 2145 - 2161

Опубликована: Июнь 20, 2024

Язык: Английский

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Single‐Cell Analysis of Endothelial Cell Injury in IgA Nephropathy

Immunity Inflammation and Disease, Год журнала: 2025, Номер 13(2)

Опубликована: Фев. 1, 2025

ABSTRACT Background The precise mechanisms responsible for renal injury in IgA nephropathy (IgAN) are not fully understood. Our study employed an extensive scRNA‐seq analysis of kidney biopsies obtained from individuals with IgAN, a specific emphasis on investigating the involvement endothelial cells. Methods We data Gene Expression Omnibus database and conducted bioinformatics analysis, which included enrichment differentially expressed genes, AUCell high‐dimensional weighted gene co‐expression network (hdWGCNA). results these analyses were further validated using human glomerular cells (HRGECs). Results ScRNA‐seq uncovered notable variations expression between IgAN control tissues. demonstrated high presence adhesion molecules components related to mitogen‐activated protein kinase signaling pathway within Furthermore, through hdWGCNA it was discovered that interleukin (IL)‐6, Rac1, cadherin exhibited associations Stimulation HRGECs IL‐6/IL‐6 receptor resulted significant reduction VE‐cad while inhibiting Rac1 led substantial decrease Rac1‐GTP levels increase expression. Conclusion This presents novel findings regarding contribution development as demonstrates IL‐6 negatively regulates via Rac1. These highlight pathogenesis IgAN.

Язык: Английский

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Anatomical considerations, testicular, and scrotal anatomy of nonobstructive azoospermia patients

Asian Journal of Andrology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Infertility, defined as the inability to conceive after 1 year of regular unprotected intercourse, impacts 10%-20% couples globally. Both male and female factors contribute equally this condition. Azoospermia, particularly nonobstructive azoospermia (NOA), which affects 10%-15% infertile men, represents a significant challenge in infertility. The advent assisted reproductive technology (ART), specifically microdissection testicular sperm extraction (micro-TESE) followed by intracytoplasmic injection (ICSI), offers possibility for men with NOA father biological children. Recent studies have focused on predictors retrieval patients, such age, volume, follicle-stimulating hormone (FSH) level. This review aims explore limited data anatomical characteristics patients provide surgical considerations micro-TESE, thereby enhancing understanding improving outcomes challenging

Язык: Английский

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Single-cell transcriptomic analysis identifies VAV3 as a critical regulator of macrophage function in gouty arthritis

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Фев. 26, 2025

Background: Gouty arthritis is a common inflammatory disease triggered by the deposition of monosodium urate (MSU)crystals in joints, leading to both acute and chronic inflammation. While macrophages have long been implicated pathogenesis gouty arthritis, exact mechanisms, differentiation conditions, key molecules involved remain unclear. Methods: Gene Set Enrichment Analysis (GSEA) was used determine primary functions macrophages. High-dimensional weighted gene co-expression network analysis (hdWGCNA), transcription factor activity analysis, pseudotemporal trajectory were applied identify VAV3 as regulating macrophage differentiation. The correlation between expression relevant biological processes further validated through Variation (GSVA) examining related bulk RNA sequencing datasets from GEO database, confirming its association with arthritis. Results: Our indicates that are crucial cell type synovial fluid microenvironment where their influenced various factors. function, positively correlated several phenotypic features progression, including angiogenesis differential across multiple database. Conclusion: findings underscore critical role novel biomarker potential therapeutic target. These results deepen our understanding suggest could broader implications other gout-related such nephropathy.

Язык: Английский

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The Codonopsis pilosula water extract improves testicular inflammatory aging in D-galactose induced aging mice by modulating the CLEC7A/inflammasome pathway

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119645 - 119645

Опубликована: Март 1, 2025

Язык: Английский

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Single-cell sequencing in non-obstructive azoospermia: insights from primary and re-analysis studies

Frontiers in Endocrinology, Год журнала: 2025, Номер 16

Опубликована: Март 19, 2025

Non-obstructive azoospermia (NOA) constitutes one of the most severe forms male infertility. Recent advancements in single-cell sequencing have significantly contributed to understanding molecular landscape NOA human testicular tissues, elucidating factors that underpin spermatogenic dysfunction. This technology has improved our condition at a cellular level. Concurrently, bioinformatics developments facilitated re-analysis publicly available datasets, offering novel insights into disorder. Nevertheless, comprehensive review integrating primary and studies is lacking. systematically evaluates 10 reporting original data samples 22 secondary re-analyzed these published data. We explore applications germ cells, Sertoli Leydig overview Our highlights findings studies, including roles transcriptional regulators, RNA transcription, endocrine disruptors, microtubular cytoskeleton, thereby bridging studies. Additionally, we discussed future research directions challenges translating clinical applications. In summary, offers high-resolution, perspective tissue, paving way for innovative therapeutic strategies

Язык: Английский

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Effects of Polystyrene Microparticles Exposures on Spermatogenic Cell Differentiation and Reproductive Endpoints in Male Mice

Environmental Pollution, Год журнала: 2025, Номер 373, С. 126200 - 126200

Опубликована: Апрель 5, 2025

Язык: Английский

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Oxylipin profiling analyses reveal that ω-3 PUFA is more susceptible to lipid oxidation in sheep testis under oxidative stress

Animal Reproduction Science, Год журнала: 2024, Номер 268, С. 107567 - 107567

Опубликована: Июль 20, 2024

Язык: Английский

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hdWGCNA and Cellular Communication Identify Active NK Cell Subtypes in Alzheimer's Disease and Screen for Diagnostic Markers through Machine Learning

Current Alzheimer Research, Год журнала: 2024, Номер 21(2), С. 120 - 140

Опубликована: Фев. 1, 2024

Background: Alzheimer's disease (AD) is a recognized complex and severe neurodegenerative disorder, presenting significant challenge to global health. Its hallmark pathological features include the deposition of β-amyloid plaques formation neurofibrillary tangles. Given this context, it becomes imperative develop an early accurate biomarker model for AD diagnosis, employing machine learning bioinformatics analysis. Methods: In study, single-cell data analysis was employed identify cellular subtypes that exhibited differences between diseased control groups. Following identification NK cells, hdWGCNA communication were conducted pinpoint cell subset with most robust effects. Subsequently, three algorithms-LASSO, Random Forest, SVM-RFE-were jointly screen modular genes highly associated AD. A logistic regression diagnostic then designed based on these characterized genes. Additionally, protein-protein interaction (PPI) networks established. Furthermore, unsupervised cluster classify genes, followed by immune infiltration in different subtypes. Finally, Spearman correlation coefficient utilized explore as well inflammatory factors. Results: We have successfully identified (RPLP2, RPSA, RPL18A) exhibit high association The nomogram provides practical assistance diagnosing predicting patients' outcomes. interconnected screened through PPI are intricately linked ribosome metabolism COVID-19 pathway. Utilizing expression unveiled distinct Particularly noteworthy subtype C3, expression, which correlates elevated levels Hence, can be inferred establishment environment patients closely intertwined heightened Conclusion: This study has not only established valuable but also delved deeply into pivotal role shaping individuals These findings offer crucial insights diagnosis patient management strategies.

Язык: Английский

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The changing landscape of nonobstructive azoospermia

Current Opinion in Urology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

Purpose of review This article aims to describe new developments in the field nonobstructive azoospermia biology, diagnostics, biomarkers, and therapeutic strategies. Recent findings studies have investigated molecular underpinnings cellular dysfunction that is contributing spermatogenic suggest abnormalities across both somatic germ cells. Biomarkers predict chances sperm retrieval are being explored utilizing cell free (cf) DNA RNA from various body fluids, addition a full range transcripts epigenetics within seminal fluid. Various approaches optimize identification surgical specimens including microfluidic machine learning approaches. Finally, regenerating production males with evolving include 3-dimensional culture techniques integration computational modeling. Summary The landscape underpinnings, technological more reliably identify novel regenerative strategies likely transform male reproduction years come.

Язык: Английский

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