Transcriptome Analysis Revealed the Affecting Mechanism of Two Diets, Trash Fish or Compound Feed, on Flesh Quality of Largemouth Bass (Micropterus salmoides)
Aquaculture Research,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
This
study
revealed
the
affecting
mechanism
of
trash
fish
(TF)
and
compound
feed
(CF)
on
flesh
quality
largemouth
bass
(
Micropterus
salmoides
)
based
muscle
transcriptome.
Largemouth
weighing
75.0
±
0.1
g
were
given
TF
or
CF
for
a
period
12
weeks.
The
group
presented
significantly
higher
efficiency
(FE)
than
p
<
0.05),
while
there
was
no
significant
difference
in
specific
growth
rate
(SGR)
between
two
groups
>
0.05).
A
total
604
differentially
expressed
genes
(DEGs)
meeting
significance
criteria
‐value
0.05
|log2foldchange|
1
identified
transcriptome
analysis.
Compared
to
group,
145
DEGs
downregulated,
459
upregulated
group.
Enrichment
analysis
Gene
Ontology
(GO)
terms
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathways
enrichment
401
GO
13
pathways,
respectively.
In
notable
increase
gene
expression
such
as
arachidonic
acid
(AA)
metabolism,
protein
processing
endoplasmic
reticulum,
cholesterol
MAPK,
focal
adhesion,
decrease
like
purine
apoptosis,
glycolysis/gluconeogenesis,
PPAR
signaling.
Real‐time
fluorescence
quantitative
PCR
results
indicated
decreased
tni
(troponin
I,
fast
skeletal
muscle‐like)
increased
stni
slow
muscle‐like),
ftni
muscle),
mustn1b
(musculoskeletal,
embryonic
nuclear
1b),
actn2b
(alpha‐actinin−2),
hspb1
(heat
shock
[HSP]
beta
1)
compared
Overall,
according
transcriptomics,
replacing
with
altered
related
meat
associated
signaling
leading
improvement
bass.
Язык: Английский
Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field
Aquaculture Reports,
Год журнала:
2025,
Номер
40, С. 102610 - 102610
Опубликована: Янв. 6, 2025
Язык: Английский
Changes in growth performance, gene expressions related to protein absorption and hepatic metabolism of triploid crucian carp (Carassius carassius triploid) caused by dietary protein level
Aquaculture Reports,
Год журнала:
2024,
Номер
35, С. 102023 - 102023
Опубликована: Март 6, 2024
The
aim
of
this
study
was
to
investigate
the
effects
dietary
protein
inclusion
level
on
growth,
absorption
pathway
and
liver
metabolism-related
in
triploid
crucian
carp
(Carassius
carassius
triploid).
Six
experimental
diets
with
different
levels
(26%,
29%,
32%,
35%,
38%,
41%)
were
formulated.
A
total
540
fish
an
initial
weight
11.79
±
0.09
g
randomly
assigned
18
cages
six
treatments
three
replicates
30
each
for
8
weeks
feeding.
results
showed
that
specific
growth
rate
(SGR)
feed
efficiency
(FE)
highly
positively
correlated
content
(p
<
0.05).
broken-line
regression
analysis
optimal
determined
be
39%
considering
SGR
carp.
In
addition,
genes
related
influenced
by
dietary.
mRNA
expressions
pept1,
lat2,
cdx2,
jak2
sp1
exhibited
similar
change
tendency
promoted
highest
expression
at
41%
groups,
32%
followed.
Furthermore,
hepatic
metabolism
tor,
s6k1,
igf1,
ampd1
adsl
reached
peak
38%
level,
while
4e-bp2
gene
level.
Finally,
igf1
cdx2
intestine
high
pearson
coefficients
suggesting
their
significant
regulated
conclusion,
evidenced
influence
pathway,
TOR
IGF
pathways
would
optimum
dietary,
which
will
lead
a
better
Язык: Английский
Effect of Dietary Digestible Protein Levels on Muscle Growth and Oxidative Stress in Amazonian Pintado (Pseudoplatystoma reticulatum × Leiarius marmoratus)
Biology,
Год журнала:
2024,
Номер
13(10), С. 825 - 825
Опубликована: Окт. 15, 2024
This
study
aimed
to
evaluate
the
effects
of
dietary
digestible
protein
levels
on
growth
dynamics
and
oxidative
stress
status
white
muscle
fibers
in
Amazonian
Pintado
(Pseudoplatystoma
reticulatum
×
Leiarius
marmoratus).
Four
hundred
fifty-five
juveniles
were
fed
diets
containing
varying
(225,
250,
275,
300,
325,
350,
or
375
g
kg−1)
for
75
days.
At
end
experiment,
fish
fasted
24
h,
anesthetized,
euthanized
obtain
samples.
The
linear
quadratic
fiber
diameter,
metabolite
concentrations,
assessed.
results
revealed
that
increasing
linearly
raised
concentrations
free
amino
acids
total
proteins
tissue
but
also
led
elevated
TBARS,
indicating
increased
stress.
Notably,
average
area
with
a
cell
greater
than
1133
µm2
decreased,
reflecting
restricted
hypertrophy,
whereas
glycogen
glucose
declined.
These
findings
suggest
although
high
enhances
acid
tissue,
it
may
compromise
hypertrophy
increase
damage
Pintado,
underscoring
complexity
optimizing
diet
formulation.
Язык: Английский
Effects of Dietary Protein and Lipid Levels on the Growth Performance and Serum Biochemical Indices of Juvenile Furong Crucian Carp
Fishes,
Год журнала:
2024,
Номер
9(11), С. 466 - 466
Опубликована: Ноя. 16, 2024
The
impact
of
dietary
protein
and
lipid
levels
on
the
growth
performance,
feed
utilization,
serum
biochemical
indices
Furong
crucian
carp
was
examined.
Five
hundred
forty
(2.35
±
0.08
g)
were
randomly
assigned
to
nine
groups
fed
diets
with
three
different
(30.0,
35.0,
40.0%)
(4.0,
7.0,
10.0%)
for
60
days.
current
findings
revealed
that
interaction
effect
between
exhibited
significance
final
average
weight
(FAW),
gain
rate
(WGR),
specific
(SGR),
efficiency
(FE),
energy
deposition
(EDR),
whole-fish
energy,
ash,
fat
content
(p
<
0.05).
Specifically,
there
a
significant
reduction
in
FAW,
WGR,
SGR
increasing
supplementation.
Conversely,
FE,
EDR,
ratios
significantly
decreased
Furthermore,
albumin
globulin
increases
response
inclusion
collectively
indicate
diet
comprising
4%
30%
optimal
utilization.
excessive
supplementation
detrimental
resulted
aggravation
metabolic
disorders.
Язык: Английский