Transcriptome Analysis Revealed the Affecting Mechanism of Two Diets, Trash Fish or Compound Feed, on Flesh Quality of Largemouth Bass (Micropterus salmoides)

Aquaculture Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

This study revealed the affecting mechanism of trash fish (TF) and compound feed (CF) on flesh quality largemouth bass ( Micropterus salmoides ) based muscle transcriptome. Largemouth weighing 75.0 ± 0.1 g were given TF or CF for a period 12 weeks. The group presented significantly higher efficiency (FE) than p < 0.05), while there was no significant difference in specific growth rate (SGR) between two groups > 0.05). A total 604 differentially expressed genes (DEGs) meeting significance criteria ‐value 0.05 |log2foldchange| 1 identified transcriptome analysis. Compared to group, 145 DEGs downregulated, 459 upregulated group. Enrichment analysis Gene Ontology (GO) terms Kyoto Encyclopedia Genes Genomes (KEGG) pathways enrichment 401 GO 13 pathways, respectively. In notable increase gene expression such as arachidonic acid (AA) metabolism, protein processing endoplasmic reticulum, cholesterol MAPK, focal adhesion, decrease like purine apoptosis, glycolysis/gluconeogenesis, PPAR signaling. Real‐time fluorescence quantitative PCR results indicated decreased tni (troponin I, fast skeletal muscle‐like) increased stni slow muscle‐like), ftni muscle), mustn1b (musculoskeletal, embryonic nuclear 1b), actn2b (alpha‐actinin−2), hspb1 (heat shock [HSP] beta 1) compared Overall, according transcriptomics, replacing with altered related meat associated signaling leading improvement bass.

Language: Английский

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Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 40, P. 102610 - 102610

Published: Jan. 6, 2025

Language: Английский

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Changes in growth performance, gene expressions related to protein absorption and hepatic metabolism of triploid crucian carp (Carassius carassius triploid) caused by dietary protein level

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 35, P. 102023 - 102023

Published: March 6, 2024

The aim of this study was to investigate the effects dietary protein inclusion level on growth, absorption pathway and liver metabolism-related in triploid crucian carp (Carassius carassius triploid). Six experimental diets with different levels (26%, 29%, 32%, 35%, 38%, 41%) were formulated. A total 540 fish an initial weight 11.79 ± 0.09 g randomly assigned 18 cages six treatments three replicates 30 each for 8 weeks feeding. results showed that specific growth rate (SGR) feed efficiency (FE) highly positively correlated content (p < 0.05). broken-line regression analysis optimal determined be 39% considering SGR carp. In addition, genes related influenced by dietary. mRNA expressions pept1, lat2, cdx2, jak2 sp1 exhibited similar change tendency promoted highest expression at 41% groups, 32% followed. Furthermore, hepatic metabolism tor, s6k1, igf1, ampd1 adsl reached peak 38% level, while 4e-bp2 gene level. Finally, igf1 cdx2 intestine high pearson coefficients suggesting their significant regulated conclusion, evidenced influence pathway, TOR IGF pathways would optimum dietary, which will lead a better

Language: Английский

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Effect of Dietary Digestible Protein Levels on Muscle Growth and Oxidative Stress in Amazonian Pintado (Pseudoplatystoma reticulatum × Leiarius marmoratus)

Biology, Journal Year: 2024, Volume and Issue: 13(10), P. 825 - 825

Published: Oct. 15, 2024

This study aimed to evaluate the effects of dietary digestible protein levels on growth dynamics and oxidative stress status white muscle fibers in Amazonian Pintado (Pseudoplatystoma reticulatum × Leiarius marmoratus). Four hundred fifty-five juveniles were fed diets containing varying (225, 250, 275, 300, 325, 350, or 375 g kg−1) for 75 days. At end experiment, fish fasted 24 h, anesthetized, euthanized obtain samples. The linear quadratic fiber diameter, metabolite concentrations, assessed. results revealed that increasing linearly raised concentrations free amino acids total proteins tissue but also led elevated TBARS, indicating increased stress. Notably, average area with a cell greater than 1133 µm2 decreased, reflecting restricted hypertrophy, whereas glycogen glucose declined. These findings suggest although high enhances acid tissue, it may compromise hypertrophy increase damage Pintado, underscoring complexity optimizing diet formulation.

Language: Английский

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Effects of Dietary Protein and Lipid Levels on the Growth Performance and Serum Biochemical Indices of Juvenile Furong Crucian Carp

Fishes, Journal Year: 2024, Volume and Issue: 9(11), P. 466 - 466

Published: Nov. 16, 2024

The impact of dietary protein and lipid levels on the growth performance, feed utilization, serum biochemical indices Furong crucian carp was examined. Five hundred forty (2.35 ± 0.08 g) were randomly assigned to nine groups fed diets with three different (30.0, 35.0, 40.0%) (4.0, 7.0, 10.0%) for 60 days. current findings revealed that interaction effect between exhibited significance final average weight (FAW), gain rate (WGR), specific (SGR), efficiency (FE), energy deposition (EDR), whole-fish energy, ash, fat content (p < 0.05). Specifically, there a significant reduction in FAW, WGR, SGR increasing supplementation. Conversely, FE, EDR, ratios significantly decreased Furthermore, albumin globulin increases response inclusion collectively indicate diet comprising 4% 30% optimal utilization. excessive supplementation detrimental resulted aggravation metabolic disorders.

Language: Английский

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