Human Monocyte-Derived Macrophages Demonstrate Distinct Responses to Ambient Particulate Matter in a Polarization State- and Particle Seasonality-Specific Manner DOI
Timothy Smyth, Stephanie A. Brocke, Yong Ho Kim

и другие.

Chemical Research in Toxicology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Macrophages are professional phagocytic immune cells that, following activation, polarize on a spectrum between the proinflammatory M1 and proresolution M2 states. have further been demonstrated to retain plasticity, allowing for reprogramming of their polarization states exposure new stimuli. Particulate matter (PM) has repeatedly shown modify macrophage function while also inducing worsening respiratory infection morbidity mortality. However, limited work considered impact initial state subsequent responses PM exposure. composition can demonstrate seasonality-specific compositional changes based differences in seasonal weather patterns energy needs, introducing need consider effects airborne when investigating its human health. This study sought determine collected during different seasons year Xinxiang, China, state-dependent manner. were differentiated using colony-stimulating factor (M-CSF) CD14+CD16– monocytes isolated from blood healthy volunteers. The resulting macrophages polarized into indicated well-characterized methods assessed function, bioenergetic properties, secretory profile single day each season year. clear phagocytic, bioenergetic, properties at baseline Specific seasonality had minimal minor effect but impacts as by enriched secretion mediator clusters particle season. Together, these data suggest that both must be function. These factors may contribute negative outcomes linked infections.

Язык: Английский

Genotoxicity and Cytotoxicity Induced In Vitro by Airborne Particulate Matter (PM2.5) from an Open-Cast Coal Mining Area DOI Creative Commons
Claudia Galeano–Páez, Hugo Brango, Karina Pastor–Sierra

и другие.

Atmosphere, Год журнала: 2024, Номер 15(12), С. 1420 - 1420

Опубликована: Ноя. 26, 2024

This study evaluates the cytotoxic and genotoxic effects of PM2.5 collected from an open-cast coal mining area in northern Colombia. Cyclohexane (CH), dichloromethane (DCM), acetone (ACE) extracts were obtained using Soxhlet extraction to isolate compounds different polarities. Human lymphocytes exposed extracted compounds, cytotoxicity genotoxicity assessed cytokinesis block micronucleus (CBMN) comet assays, incorporating FPG ENDO III enzymes detect oxidative DNA damage. Chemical analysis revealed that organic fractions consisted mainly modified hydrocarbons volatile compounds. The CBMN assay showed a significant increase micronuclei binucleated (MNBN) mononucleated (MNMONO) cells nucleoplasmic bridges (NPB) lymphocytes. substantial damage, particularly with ACE extract, which significantly increased oxidized purines pyrimidines. DCM induced similar effects, while CH moderate effects. CREST immunostaining aneugenic activity, extracts. These results suggest polar PM2.5, likely containing metals PAHs, contribute damage chromosomal instability. highlights need monitor composition regions implement stricter environmental policies reduce exposure health risks.

Язык: Английский

Процитировано

1
Human Monocyte-Derived Macrophages Demonstrate Distinct Responses to Ambient Particulate Matter in a Polarization State- and Particle Seasonality-Specific Manner DOI
Timothy Smyth, Stephanie A. Brocke, Yong Ho Kim

и другие.

Chemical Research in Toxicology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Macrophages are professional phagocytic immune cells that, following activation, polarize on a spectrum between the proinflammatory M1 and proresolution M2 states. have further been demonstrated to retain plasticity, allowing for reprogramming of their polarization states exposure new stimuli. Particulate matter (PM) has repeatedly shown modify macrophage function while also inducing worsening respiratory infection morbidity mortality. However, limited work considered impact initial state subsequent responses PM exposure. composition can demonstrate seasonality-specific compositional changes based differences in seasonal weather patterns energy needs, introducing need consider effects airborne when investigating its human health. This study sought determine collected during different seasons year Xinxiang, China, state-dependent manner. were differentiated using colony-stimulating factor (M-CSF) CD14+CD16– monocytes isolated from blood healthy volunteers. The resulting macrophages polarized into indicated well-characterized methods assessed function, bioenergetic properties, secretory profile single day each season year. clear phagocytic, bioenergetic, properties at baseline Specific seasonality had minimal minor effect but impacts as by enriched secretion mediator clusters particle season. Together, these data suggest that both must be function. These factors may contribute negative outcomes linked infections.

Язык: Английский

Процитировано

0