The Dose-Dependent Effects of Fluorocitrate on the Metabolism and Activity of Astrocytes and Neurons DOI Creative Commons
Heng Zhuang,

Deliang Yuan,

Fuxiu Shi

и другие.

Brain Sciences, Год журнала: 2025, Номер 15(2), С. 99 - 99

Опубликована: Янв. 21, 2025

Background: Fluorocitrate (FC) ranging from 5 μM to mM is often used as a specific metabolic inhibitor of the astrocytes study astrocytic functions. Whether FC at such concentrations may affect neuronal metabolism and function in vivo remains unclear. Methods: We examined effects on ATP levels Ca2+ activity neurons motor cortices living mice using two-photon microscopy. Results: found that 25 250 decreased intracellular cortex. Equally, FC, but not reduced dendritic processes layer pyramidal neurons. However, increased activity, whereas ≥250 it. To test whether differential reflect direct effect or its indirect astrocytes, we CNO-hM3Dq chemogenetic approach block FC. In absence still neurons, respectively, suggesting inhibiting activity. Further, μM, size spines over 2 h. Conclusions: Our findings suggest high (≥250 μM) functions, it directly affects synaptic plasticity vivo.

Язык: Английский

Sleep, Glial Function, and the Endocannabinoid System: Implications for Neuroinflammation and Sleep Disorders DOI Open Access
Josué Camberos-Barraza, Alejandro Camacho-Zamora,

José Candelario Bátiz-Beltrán

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3160 - 3160

Опубликована: Март 9, 2024

The relationship between sleep, glial cells, and the endocannabinoid system represents a multifaceted regulatory network with profound implications for neuroinflammation cognitive function. molecular underpinnings of sleep modulation by its influence on cell activity are discussed, shedding light reciprocal relationships that govern these processes. Emphasis is placed understanding role cells in mediating neuroinflammatory responses their patterns. Additionally, this review examines how interfaces glia-immune signaling to regulate inflammatory cascades within central nervous system. Notably, consequences disrupted neuroinflammation, dysfunction addressed, encompassing neurodegenerative disorders, mood disturbances, decline. Insights into bidirectional function context explored, providing comprehensive perspective potential mechanisms underlying impairments associated disturbances. Furthermore, therapeutic avenues targeting mitigate restore homeostasis, normalize identification novel targets intricate holds promise addressing conditions characterized dysfunction. This work aims examine complexities neural regulation identify intervention.

Язык: Английский

Процитировано

6

The Dose-Dependent Effects of Fluorocitrate on the Metabolism and Activity of Astrocytes and Neurons DOI Creative Commons
Heng Zhuang,

Deliang Yuan,

Fuxiu Shi

и другие.

Brain Sciences, Год журнала: 2025, Номер 15(2), С. 99 - 99

Опубликована: Янв. 21, 2025

Background: Fluorocitrate (FC) ranging from 5 μM to mM is often used as a specific metabolic inhibitor of the astrocytes study astrocytic functions. Whether FC at such concentrations may affect neuronal metabolism and function in vivo remains unclear. Methods: We examined effects on ATP levels Ca2+ activity neurons motor cortices living mice using two-photon microscopy. Results: found that 25 250 decreased intracellular cortex. Equally, FC, but not reduced dendritic processes layer pyramidal neurons. However, increased activity, whereas ≥250 it. To test whether differential reflect direct effect or its indirect astrocytes, we CNO-hM3Dq chemogenetic approach block FC. In absence still neurons, respectively, suggesting inhibiting activity. Further, μM, size spines over 2 h. Conclusions: Our findings suggest high (≥250 μM) functions, it directly affects synaptic plasticity vivo.

Язык: Английский

Процитировано

0