Engineering of 4-hydroxyphenylacetate 3-hydroxylase derived from Pseudomonas aeruginosa for the ortho-hydroxylation of ferulic acid
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
264, С. 130545 - 130545
Опубликована: Фев. 29, 2024
Язык: Английский
Secondary metabolites from marine fungus Penicillium chrysogenum VH17 and their antimicrobial and cytotoxic potential
Bioscience Biotechnology and Biochemistry,
Год журнала:
2024,
Номер
88(11), С. 1254 - 1260
Опубликована: Авг. 16, 2024
ABSTRACT
One
new
compound,
methyl
3-((1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)amino)-3-oxopropanoate
(1),
along
with
9
known
secondary
metabolites
(2-10)
were
isolated
and
elucidated
chemical
structures
from
the
methanol
extract
of
marine-derived
fungus
Penicillium
chrysogenum
VH17.
Subsequent
bioassays
showed
antimicrobial
cytotoxic
potential
compounds.
All
compounds
1-10
displayed
effects
against
at
least
one
tested
reference
microorganism
MIC
values
ranging
32
to
256
µg
mL−1.
Furthermore,
compound
4
exhibited
significant
cytotoxicity
all
cell
lines,
HepG2,
A549,
MCF7
IC50
29.43
±
1.37,
33.02
1.53,
36.72
1.88
µM,
respectively,
whereas
3
weak
HepG2
lines
87.17
6.31
97.32
5.66
respectively.
Язык: Английский
Comparative analysis of substrate- and regio-selectivity of HpaB monooxygenases and their application to hydroxydaidzein synthesis
Journal of Biotechnology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 1, 2024
4-Hydroxyphenylacetate
3-hydroxylase
(HpaB)
has
high
potential
for
use
in
polyphenol
synthesis
via
ortho-hydroxylation.
Although
the
HpaB
enzymes
from
Pseudomonas
aeruginosa
(PaHpaB)
and
Escherichia
coli
(EcHpaB)
have
been
well
studied,
few
studies
compared
their
activity
substrate
selectivity.
Thus,
which
is
optimal
biotechnological
production
of
polyphenols
unclear.
In
this
study,
we
performed
a
comparative
analysis
substrate-
regio-selectivity
PaHpaB,
EcHpaB,
recently
discovered
enzyme
Rhodococcus
opacus
(RoHpaB).
The
these
was
first
toward
representative
aromatic
substrates.
PaHpaB
EcHpaB
exhibited
very
similar
catalytic
p-coumaric
acid
tyrosol
with
one
benzene
ring,
whereas
greater
than
resveratrol
naringenin
two
rings.
These
results
suggest
that
superior
to
converting
bulky
compounds.
Furthermore,
also
flavonoid,
daidzein
(7,4'-dihydroxyisoflavone),
did
not.
RoHpaB
strong
addition
other
Interestingly,
hydroxylated
6-position
daidzein,
3'-position.
enabled
facile
not
only
6-hydroxydaidzein
3'-hydroxydaidzein
but
6,3'-dihydroxydaidzein
cascade
reaction.
This
study
demonstrate
hydroxydaidzeins
using
enzymes.
Язык: Английский