Journal of Evolutionary Biochemistry and Physiology, Год журнала: 2024, Номер 60(6), С. 2191 - 2214
Опубликована: Ноя. 1, 2024
Язык: Английский
Complex Issues of Cardiovascular Diseases, Год журнала: 2025, Номер 13(4), С. 191 - 203
Опубликована: Янв. 11, 2025
Highlights A fundamental distinguishing feature of normal endothelial cell morphotypes from pathological ones is their preserved orientation along the direction blood flow in absence cytoplasmic or membrane defects. The main characteristics dysfunctional cells include a spherical shape (indicating loss cellular flow), presence large vacuoles within cell, vacuolization, impaired plasma integrity, reduced contrast between nucleus and cytoplasm, partial detachment basement membrane. condition organelles (mitochondria, Golgi complex, endoplasmic reticulum) integrity are not sensitive specific markers endothelium compared to aforementioned features Aim. To analyze electron microscopic using descending aorta rats (characterized by laminar flow). Methods. study was conducted on 5 male Wistar (age ≈ 6 months, body weight 500 g). extracted aortas were chemically fixed 2.5% glutaraldehyde, post-fixed 1% osmium tetroxide solution with 1.5% potassium ferrocyanide, incubated thiocarbohydrazide, stained 2% aqueous solution, contrasted phosphotungstic acid, gadolinium triacetate, dehydrated ascending concentrations ethanol, isopropanol, acetone, embedded mixture acetone epoxy resin, then pure Araldite 502 followed its polymerization. After grinding polishing, samples lead citrate, coated carbon, visualized backscattered scanning microscopy. Results. Electron analysis identified three cells: 1) elongated an nucleus; semicircular oval, round, kidney-shaped, polymorphic less pronounced but clearly visible flow; 3) even flow. often contained various Dysfunctional also exhibited several morphotypes, characterized different combinations following features: flow, disruption However, changes structure adjacent observed cells. Conclusion. preservation indicates phenotype other signs (vacuoles membrane).
Язык: Английский
Процитировано
0
PLoS ONE, Год журнала: 2025, Номер 20(3), С. e0316383 - e0316383
Опубликована: Март 13, 2025
Hypertension in the elderly can seriously lead to cerebral microvascular damage and promote development of vascular cognitive impairment. While endothelial function is crucial protection, it unclear whether aging exacerbates hypertension-induced dysfunction through dysfunction. In this study, we injected D-galactose (D-gal) into 24 spontaneous hypertension rats (SHR) Wistar-Kyoto (WKY) for 12 weeks induce aging. Firstly, results behavioral experiments showed that compared with WKY SHRs D-gal 0 week, had more severe memory Subsequently, pathological changes brain microvessels their structural functional were significant. After that, molecular enormous indicators (nitric oxide (NO), endothelin (ET-1), platelet cell adhesion molecule-1(CD31) tight junction protein), aggravation blood-brain barrier (BBB) damage, microglial activation upregulation pro-inflammatory cytokines. Ultimately, combination treatment nimodipine butylphthalide two drugs could hugely improve SHRs. summary, elaborated SHRs, which may be due dysfunction, even BBB neuroinflammation, while providing a theoretical basis hypertension-related diseases.
Язык: Английский
Процитировано
0
Journal of Alzheimer s Disease, Год журнала: 2025, Номер unknown
Опубликована: Апрель 22, 2025
Background Cerebral amyloid angiopathy (CAA) is a form of cerebral small vessel disease (SVD) associated with Alzheimer's disease, intracerebral hemorrhage, and cognitive decline. Despite its clinical significance, no reliable serum biomarker exists for early diagnosis or monitoring progression. Objective This study hypothesizes that α1-acid glycoprotein (α1-AGP) other biomarkers can aid CAA assessment using gel-based mass spectrometry. A comparative analysis was performed to investigate associations between radiological scores. Methods Serum proteins from individuals probable possible (n = 10), classified the modified Boston criteria, age-matched controls 10) were analyzed via two-dimensional differential gel electrophoresis (2D-DIGE) matrix-assisted laser desorption/ionization time-of-flight tandem spectrometry (MALDI-TOF/TOF-MS). Candidate validated enzyme-linked immunosorbent assay (ELISA). Outcome measures included diagnostic accuracy, assessed by receiver operating characteristic (ROC) curve analysis, correlations α1-AGP levels CAA-SVD Results Four proteins—hemopexin, complement C3, C9, α1-AGP—were significantly elevated, while apolipoprotein A-1 reduced in group. ELISA confirmed higher (p < 0.0001). ROC demonstrated could indicate presence sensitivity specificity 1.00 (95%CI: 1.000, 1.000). Additionally, correlated score (R² 0.783). Conclusions may serve as novel CAA. Larger cohorts external validation are required substantiate these findings determine their relevance.
Язык: Английский
Процитировано
0
Thrombosis Research, Год журнала: 2024, Номер 243, С. 109154 - 109154
Опубликована: Сен. 17, 2024
Язык: Английский
Процитировано
1
Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(11), С. 13058 - 13077
Опубликована: Ноя. 17, 2024
Coronary artery bypass grafting (CABG) using cardiopulmonary (CPB) causes a systemic inflammatory response that can worsen patient outcomes. Off-pump surgery has been associated with reduced response. The precise mechanisms and the role of extracellular vesicles (EVs) in this context are not fully understood. This study aimed to investigate early immune response, including main T- B-lymphocyte subsets, cytokine profiles, plasma EVs, patients undergoing off-pump (
Язык: Английский
Процитировано
1
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12627 - 12627
Опубликована: Ноя. 25, 2024
Albumin is a major protein in mammalian blood plasma or serum, where its concentration healthy organisms about 600 μM [...].
Язык: Английский
Процитировано
1
Journal of College of Physicians And Surgeons Pakistan, Год журнала: 2024, Номер unknown, С. 790 - 794
Опубликована: Июль 1, 2024
Objective: To investigate the factors contributing to blockage of perfusion tubes during hyperthermic intraperitoneal chemotherapy (HIPEC) in patients with appendiceal pseudomyxoma peritonei (PMP) undergoing combined cytoreductive surgery (CRS) and treatment, construct a nomogram for predicting risk tube occlusion.Study Design: Observational study.
Язык: Английский
Процитировано
0
New discovery., Год журнала: 2024, Номер unknown, С. 1 - 25
Опубликована: Ноя. 20, 2024
Cerebral ischemia primarily results from vascular stenosis or blockage, which activates inflammatory cells and triggers an immune response. An excessive response can exacerbate the damage caused by cerebral ischemia. In this review, keywords “immune response” “cerebral ischemia” were entered into PubMed database, yielding 241 articles, of 141 included in analysis. Relevant literature 2021 to 2024 was summarized, classified, synthesized delineate advancements field. Consequently, exploring basic physiology responses brain injury, we found that microglia phagocytose dead neurons, thereby ameliorating ischemic injury. However, accumulate attack blood vessels nerve following ischemia, resulting additional damage. As a result, targeting CD8 T cells, astrocytes, superoxide dismutase (SOD), interleukin-10 (IL-10), tumor necrosis factor (TNF), NLRP3, NF-κB signaling pathway help mitigate Furthermore, specific mechanisms efficacy therapeutic drugs recent years analyzed, revealing their potential repair blood-brain barrier, endothelial while also reducing infarct size responses. Together, highlight particularly microglia, present new breakthroughs neuron phagocytosis, improvement responses, reduction These findings provide clinicians researchers with cutting-edge references for treatment strategies.
Язык: Английский
Процитировано
0
Journal of Evolutionary Biochemistry and Physiology, Год журнала: 2024, Номер 60(6), С. 2191 - 2214
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
0