Annals of 3D Printed Medicine,
Год журнала:
2024,
Номер
15, С. 100161 - 100161
Опубликована: Июнь 22, 2024
Additive
manufacturing
techniques
capable
of
fabricating
biocompatible
scaffolds
with
a
given
submicron/micron/supramicron
structure
are
growing
interest
for
biomedical
applications,
including
tissue
engineering
and
tumor
biology
studies.
Here,
we
propose
antisolvent
3D
printing
electrospinning
to
obtain
biopolymer
different
structural,
mechanical,
surface
properties
compare
the
cultivation
patterns
glioblastoma
cells.
We
found
that
human
G01
cells,
derived
from
tissue,
were
able
colonize
in
time-dependent
manner;
cells
showed
high
viability
as
confirmed
by
colorimetric
MTT
assay,
confocal
fluorescence
microscopy,
scanning
electron
microscopy
data.
Electrospun
collagen
(low
porosity,
thin
2.75±0.22
μm
fibers,
low
Young's
modulus
0.076±0.033
MPa)
provided
monolayer-like
growth
dense
cell-cell
contacts,
while
3D-printed
PLGA
(high
thick
∼150
µm
18±2
stimulated
glioblastoma-specific
spindle-like
morphology.
All
non-toxic
maintained
cell
at
least
2
weeks.
The
developed
could
be
further
used
research
model
vitro
or
brain
injury.
Cancers,
Год журнала:
2025,
Номер
17(7), С. 1122 - 1122
Опубликована: Март 27, 2025
(1)
Background:
Glioblastoma
(GBM)
is
the
most
aggressive
and
common
primary
malignant
brain
tumor
in
adults,
constituting
45.6%
of
tumors.
We
explored
impact
gene
methylation
O-6-Methylguanine-DNA
Methyltransferase
(MGMT)
Transforming
Growth
Factor
Beta
(TGFB)
complex
using
TCGA
dataset
for
GBM
patients.
(2)
Methods:
implemented
a
multivariate
Cox
proportional
hazards
model
to
directly
compare
hazard
ratios
TGFB1/2/3
MGMT
relation
OS,
considering
male
versus
female,
age
at
diagnosis,
interactions
with
TGFB2
sex
variables.
Reactome
analysis
was
performed
identify
enriched
pathways
negatively
correlated
methylation.
(3)
Results:
The
patients
had
high
levels
methylation;
this
primarily
benefited
young
adult
patients,
exhibited
significantly
improved
OS
prognosis
HR
(95%
CI
range)
=
0.04
(0.006–0.274);
p
0.001)
relative
TGFB1highMe
(HR
0.657
(0.454–0.951);
0.026)
MGMThighMe
0.667
(0.475–0.936);
0.019)
groups
collectively
represented
T-cell
activation,
differentiation,
effector
functions,
antigen
presentation,
Toll-like
receptor
pathways.
Gene
level
mRNA
expression
highlighted
four
positive
prognostic
genes
upregulated
tissues,
their
validated
independent
single-cell
RNA-seq
experiments.
These
were
highly
expressed
macrophages
(HIF1A,
TRIM22,
IRAK4,
PARP9).
In
contrast,
MALT1
only
product
negative
on
1.997
(1.1–3.625);
0.023).
(4)
Conclusions:
Increased
predict
especially
above
that
methylation,
should
be
considered
during
administration
mRNA-based
therapies.
Biomedicines,
Год журнала:
2024,
Номер
12(12), С. 2664 - 2664
Опубликована: Ноя. 22, 2024
Glioblastoma
multiforme
(GBM),
a
WHO
grade
4
glioma,
is
the
most
common
and
aggressive
primary
brain
tumor,
characterized
by
rapid
progression
poor
prognosis.
The
heterogeneity
of
GBM
complicates
diagnosis
treatment,
driving
research
into
molecular
biomarkers
that
can
offer
insights
tumor
behavior
guide
personalized
therapies.
This
review
explores
recent
advances
in
biomarkers,
highlighting
their
potential
to
improve
treatment
outcomes
patients.
Key
such
as
MGMT
promoter
methylation,
IDH1/2
mutations,
EGFR
amplification,
TERT
etc.,
are
examined
for
roles
prognosis,
therapeutic
response,
classification.
While
valuable
tailoring
treatments,
clinical
application
hindered
heterogeneity,
dynamic
genetic
evolution,
lack
standardized
testing
methods.
Future
should
aim
confirm
new
incorporate
them
regular
practice
prognosis
choices.
Advances
genomic
proteomic
technologies,
along
with
consistent
biomarker
detection,
could
transform
care
enhance
patient
outcomes.
Annals of 3D Printed Medicine,
Год журнала:
2024,
Номер
15, С. 100161 - 100161
Опубликована: Июнь 22, 2024
Additive
manufacturing
techniques
capable
of
fabricating
biocompatible
scaffolds
with
a
given
submicron/micron/supramicron
structure
are
growing
interest
for
biomedical
applications,
including
tissue
engineering
and
tumor
biology
studies.
Here,
we
propose
antisolvent
3D
printing
electrospinning
to
obtain
biopolymer
different
structural,
mechanical,
surface
properties
compare
the
cultivation
patterns
glioblastoma
cells.
We
found
that
human
G01
cells,
derived
from
tissue,
were
able
colonize
in
time-dependent
manner;
cells
showed
high
viability
as
confirmed
by
colorimetric
MTT
assay,
confocal
fluorescence
microscopy,
scanning
electron
microscopy
data.
Electrospun
collagen
(low
porosity,
thin
2.75±0.22
μm
fibers,
low
Young's
modulus
0.076±0.033
MPa)
provided
monolayer-like
growth
dense
cell-cell
contacts,
while
3D-printed
PLGA
(high
thick
∼150
µm
18±2
stimulated
glioblastoma-specific
spindle-like
morphology.
All
non-toxic
maintained
cell
at
least
2
weeks.
The
developed
could
be
further
used
research
model
vitro
or
brain
injury.
