Xist RNA binds select autosomal genes and depends on Repeat B to regulate their expression DOI Open Access
Shengze Yao,

Yesu Jeon,

Barry Kesner

и другие.

Опубликована: Окт. 18, 2024

Xist, a pivotal player in X chromosome inactivation (XCI), has long been perceived as cis-acting noncoding RNA that binds exclusively to the inactive (Xi). However, Xist’s ability diffuse under select circumstances also documented, leading us suspect Xist may have targets and functions beyond Xi. Here, using female mouse embryonic stem cells (ES) fibroblasts (MEF) models, we demonstrate indeed can localize its binding is limited ∼100 genes undergoing XCI (ES cells) post-XCI (MEFs). The target are diverse function but unified by their active chromatin status. discretely promoters of neighborhoods relatively depleted for Polycomb marks, contrasting with broad, Polycomb-enriched domains reported human XIST RNA. We find associated down-modulation autosomal gene expression. unlike on Xi, does not lead full silencing spread gene. Over-expressing transgenic ES similarly suppression, while deleting Repeat B motif reduces perturbs down-regulation. Furthermore, treating inhibitor, X1, leads loss suppression. Altogether, our findings reveal identify crucial domain in-trans mice, indicate targeting be disrupted X1 small molecule inhibitor.

Язык: Английский

Developmental Programming: Sex-specific Effects of Prenatal Exposure to a Real-Life Mixture of Environmental Chemicals on Liver Function and Transcriptome in Sheep. DOI
Soundara Viveka Thangaraj, Michelle Bellingham, Richard G. Lea

и другие.

Environmental Pollution, Год журнала: 2025, Номер 367, С. 125630 - 125630

Опубликована: Янв. 5, 2025

Язык: Английский

Процитировано

0
Control of Mycobacterium tuberculosis infection in the elderly: Is there a role for epigenetic reprogramming reversal? DOI
Dámaris P. Romero‐Rodríguez,

Carlos A. Díaz‐Alvarado,

Héctor Isaac Rocha-­González

и другие.

BioFactors, Год журнала: 2025, Номер 51(1)

Опубликована: Янв. 1, 2025

Abstract With the increase in elderly population worldwide, number of subjects suffering from tuberculosis (TB) has shown an increased prevalence this group. Immunosenescence is essential phenomenon because it may reactivate lesions and render their adaptive immunity dysfunctional. In addition, inflammation lungs also Although effective drugs are available, they often tolerated inadequately, reducing adherence to therapy leading therapeutic failure. Comorbidities, poor general health status, other medications lead drug adverse reactions reduced treatment elderly. Hence, older adults require individualized approach for better outcomes. Trained immunity, which involves epigenetic reprogramming, contribute balancing dysfunction innate people. This review analyzes relationship between inflammation, age, Mycobacterium . Moreover, we hypothesize that immunomodulation using trained activators will help reduce while enhancing antimicrobial responses Understanding immunomodulation's molecular physiological effects informed decisions about TB prevention strategies uniquely designed

Язык: Английский

Процитировано

0
Sirtuin 3 drives sex-specific responses to age-related changes in mouse embryonic fibroblasts DOI

Ena Šimunić,

Iva I. Podgorski, Marija Pinterić

и другие.

Mechanisms of Ageing and Development, Год журнала: 2024, Номер 222, С. 111996 - 111996

Опубликована: Окт. 11, 2024

Язык: Английский

Процитировано

1
Xist RNA binds select autosomal genes and depends on Repeat B to regulate their expression DOI Open Access
Shengze Yao,

Yesu Jeon,

Barry Kesner

и другие.

Опубликована: Окт. 18, 2024

Xist, a pivotal player in X chromosome inactivation (XCI), has long been perceived as cis-acting noncoding RNA that binds exclusively to the inactive (Xi). However, Xist’s ability diffuse under select circumstances also documented, leading us suspect Xist may have targets and functions beyond Xi. Here, using female mouse embryonic stem cells (ES) fibroblasts (MEF) models, we demonstrate indeed can localize its binding is limited ∼100 genes undergoing XCI (ES cells) post-XCI (MEFs). The target are diverse function but unified by their active chromatin status. discretely promoters of neighborhoods relatively depleted for Polycomb marks, contrasting with broad, Polycomb-enriched domains reported human XIST RNA. We find associated down-modulation autosomal gene expression. unlike on Xi, does not lead full silencing spread gene. Over-expressing transgenic ES similarly suppression, while deleting Repeat B motif reduces perturbs down-regulation. Furthermore, treating inhibitor, X1, leads loss suppression. Altogether, our findings reveal identify crucial domain in-trans mice, indicate targeting be disrupted X1 small molecule inhibitor.

Язык: Английский

Процитировано

1
Xist RNA binds select autosomal genes and depends on Repeat B to regulate their expression DOI Open Access
Shengze Yao,

Yesu Jeon,

Barry Kesner

и другие.

Опубликована: Дек. 5, 2024

Xist, a pivotal player in X chromosome inactivation (XCI), has long been perceived as cis-acting noncoding RNA that binds exclusively to the inactive (Xi). However, Xist’s ability diffuse under select circumstances also documented, leading us suspect Xist may have targets and functions beyond Xi. Here, using female mouse embryonic stem cells (ES) fibroblasts (MEF) models, we demonstrate indeed can localize its binding is limited ∼100 genes undergoing XCI (ES cells) post-XCI (MEFs). The target are diverse function but unified by their active chromatin status. discretely promoters of neighborhoods relatively depleted for Polycomb marks, contrasting with broad, Polycomb-enriched domains reported human XIST RNA. We find associated down-modulation autosomal gene expression. unlike on Xi, does not lead full silencing spread gene. Over-expressing transgenic ES similarly leads suppression, while deleting Repeat B motif reduces perturbs down-regulation. Furthermore, treating inhibitor, X1, loss suppression. Altogether, our findings reveal identify crucial domain in-trans mice, indicate targeting be disrupted small molecule inhibitor.

Язык: Английский

Процитировано

1
Novel insights into the activating transcription factor 4 in Alzheimer’s disease and associated aging-related diseases: Mechanisms and therapeutic implications DOI
Nan Zhang, Jianfei Nao,

Shun Zhang

и другие.

Frontiers in Neuroendocrinology, Год журнала: 2024, Номер 74, С. 101144 - 101144

Опубликована: Май 24, 2024

Язык: Английский

Процитировано

0
Xist RNA binds select autosomal genes and depends on Repeat B to regulate their expression DOI Creative Commons
Shengze Yao,

Yesu Jeon,

Barry Kesner

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 23, 2024

Xist, a pivotal player in X chromosome inactivation (XCI), has long been perceived as cis-acting noncoding RNA that binds exclusively to the inactive (Xi). However, Xists ability diffuse under select circumstances also documented, leading us suspect Xist may have targets and functions beyond Xi. Here, using female mouse embryonic stem cells (ES) fibroblasts (MEF) models, we demonstrate indeed can localize its binding is limited approximately 100 genes undergoing XCI (ES cells) post-XCI (MEFs). The target are diverse function but unified by their active chromatin status. discretely promoters of neighborhoods relatively depleted for Polycomb marks, contrasting with broad, Polycomb-enriched domains reported human XIST RNA. We find associated down-modulation autosomal gene expression. unlike on Xi, does not lead full silencing spread gene. Over-expressing transgenic ES similarly leads suppression, while deleting Repeat B motif reduces perturbs down-regulation. Furthermore, treating inhibitor, X1, loss suppression. Altogether, our findings reveal ~100 identify crucial domain in-trans mice, indicate targeting be disrupted small molecule inhibitor.

Язык: Английский

Процитировано

0
Elucidating the Role of Sirtuin 3 in Mammalian Oocyte Aging DOI Creative Commons
Paweł Kordowitzki

Cells, Год журнала: 2024, Номер 13(18), С. 1592 - 1592

Опубликована: Сен. 22, 2024

The field of reproductive biology has made significant progress in recent years, identifying specific molecular players that influence oocyte development and function. Among them, sirtuin 3 (SIRT3) attracted particular attention for its central role mediating mitochondrial function cellular stress responses oocytes. So far, studies have demonstrated the knockdown SIRT3 leads to a decrease blastocyst formation an increase oxidative within embryo, underscoring importance maintaining redox balance critical embryonic survival growth. Furthermore, literature reveals signaling pathways, such as SIRT3- Glycogen synthase kinase-3 beta (GSK3β) deacetylation pathway, crucial mitigating stress-related anomalies meiosis, particularly under conditions like maternal diabetes. Overall, emerging regulating highlights understanding intricate connections between metabolism, response overall health This knowledge could lead novel strategies support quality fertility, with far-reaching implications assisted technologies women's healthcare. commentary aims provide overview oocytes by synthesizing results from multitude studies. aim is elucidate development, maturation, aging identify areas where further research needed.

Язык: Английский

Процитировано

0
Characterization and Physiological Differences of Two Primary Cultures of Human Normal and Hypertrophic Scar Dermal Fibroblasts: A Pilot Study DOI Creative Commons
Natalia Yudintceva, Yulia V. Kolesnichenko, Alla Shatrova

и другие.

Biomedicines, Год журнала: 2024, Номер 12(10), С. 2295 - 2295

Опубликована: Окт. 10, 2024

Dermal fibroblasts (DFs) are key participants in skin hypertrophic scarring, and their properties being studied to identify the molecular cellular mechanisms underlying pathogenesis of scarring.

Язык: Английский

Процитировано

0
Xist RNA binds select autosomal genes and depends on Repeat B to regulate their expression DOI Open Access
Shengze Yao,

Yesu Jeon,

Barry Kesner

и другие.

Опубликована: Окт. 18, 2024

Xist, a pivotal player in X chromosome inactivation (XCI), has long been perceived as cis-acting noncoding RNA that binds exclusively to the inactive (Xi). However, Xist’s ability diffuse under select circumstances also documented, leading us suspect Xist may have targets and functions beyond Xi. Here, using female mouse embryonic stem cells (ES) fibroblasts (MEF) models, we demonstrate indeed can localize its binding is limited ∼100 genes undergoing XCI (ES cells) post-XCI (MEFs). The target are diverse function but unified by their active chromatin status. discretely promoters of neighborhoods relatively depleted for Polycomb marks, contrasting with broad, Polycomb-enriched domains reported human XIST RNA. We find associated down-modulation autosomal gene expression. unlike on Xi, does not lead full silencing spread gene. Over-expressing transgenic ES similarly suppression, while deleting Repeat B motif reduces perturbs down-regulation. Furthermore, treating inhibitor, X1, leads loss suppression. Altogether, our findings reveal identify crucial domain in-trans mice, indicate targeting be disrupted X1 small molecule inhibitor.

Язык: Английский

Процитировано

0