Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis DOI Creative Commons
Letícia Gomes de Pontes, Lucila Akune Barreiros, Lillian Nunes Gomes

и другие.

Biomolecules, Год журнала: 2024, Номер 15(1), С. 32 - 32

Опубликована: Дек. 30, 2024

Background: In this study, we investigated the role of extracellular vesicles (EVs) in pathogenesis Congenital Zika Syndrome (CZS). Previous studies have highlighted EVs intercellular communication and modulation biological processes during viral infections, motivating our in-depth analysis. Our objective was to identify specific molecular signatures patients with CZS, focusing on their potential as biomarkers cellular pathways affected by infection. Methods: We conducted advanced proteomic metabolomic analyses using mass spectrometry for protein metabolite identification. were isolated from CZS patient samples control groups Izon qEV size-exclusion chromatography columns. Results: The analyzed presented distinct profiles CZS. Proteomic analysis revealed significant alterations proteins, suggesting involvement PI3K-AKT-mTOR pathway, while metabolomics metabolites related critical virus pathogenesis. These findings suggest a key pathway regulating infection indicate communication. Additionally, results identified capable aiding early diagnosis assessing disease progression. Conclusions: This study demonstrates that play crucial identification highlights possible therapeutic target, providing new opportunities development more effective treatment strategies significantly advance understanding underscore need further investigations techniques validate explore these targets.

Язык: Английский

Unveiling the Wonders of Bacteria-Derived Extracellular Vesicles: From Fundamental Functions to Beneficial Applications DOI Creative Commons

Mariam Rima,

Mariam Dakramanji,

E. Hayek

и другие.

Heliyon, Год журнала: 2025, Номер 11(4), С. e42509 - e42509

Опубликована: Фев. 1, 2025

:Extracellular vesicles (EVs), are critical mediators of intercellular communication and exhibit significant potential across various biomedical domains. These nano-sized, membrane-encapsulated entities have captured substantial interest due to their diverse roles in pathogenesis promising therapeutic applications. EVs manage numerous physiological processes by transferring bioactive molecules, including proteins, lipids, nucleic acids, between cells. This review delves into the factors influencing properties EVs, such as temperature stress conditions, which collectively influence size, composition, functional attributes. We also describe emerging emphasizing involvement microbial interactions, immune modulation, antimicrobial resistance spread innovative diagnostic instruments. Despite applications, advancement EV-based therapies faces several challenges, will be discussed. By elucidating these elements, we aim provide a comprehensive overview transformative revolutionizing diagnostics therapeutics medicine.

Язык: Английский

Процитировано

4
Review on bacterial outer membrane vesicles: structure, vesicle formation, separation and biotechnological applications DOI Creative Commons
Xiaofei Zhao,

Yusen Wei,

Yuqing Bu

и другие.

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Янв. 21, 2025

Outer membrane vesicles (OMVs), shed by Gram-negative bacteria, are spherical nanostructures that play a pivotal role in bacterial communication and host-pathogen interactions. Comprising an outer envelope encapsulating variety of bioactive molecules from their progenitor OMVs facilitate material informational exchange. This review delves into the recent advancements OMV research, providing comprehensive overview structure, biogenesis, mechanisms vesicle formation. It also explores pathogenicity techniques for enrichment isolation. Furthermore, highlights burgeoning applications field biomedicine, emphasizing potential as diagnostic tools, vaccine candidates, drug delivery vectors.

Язык: Английский

Процитировано

3
Consistency in bacterial extracellular vesicle production: key to their application in human health DOI Open Access
Ke Dai, Bo Liao, Xiaotian Huang

и другие.

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2025, Номер 6(1), С. 1 - 20

Опубликована: Янв. 17, 2025

Bacterial extracellular vesicles (BEVs) are naturally occurring functional structures that play critical roles in bacterial life processes. These vesicles, commonly known as outer membrane (OMVs), were first found to be released by Gram-negative bacteria; however, it has since been confirmed Gram-positive bacteria also secrete BEVs. As research advances, BEVs increasingly utilized diverse applications, including vaccine development and drug delivery. Nevertheless, the effective employment of these contexts requires acquisition with consistent properties functions through appropriate culture, isolation, purification methods. This review examines advantages disadvantages various techniques alongside heterogeneity they may introduce. We utilize a framework critically analyze barriers their application factors influencing characteristics. Additionally, we constructively propose solutions enhance consistency BEVs, thereby facilitating further application.

Язык: Английский

Процитировано

1
Extracellular Vesicles of Minimalistic Mollicutes as Mediators of Immune Modulation and Horizontal Gene Transfer DOI Creative Commons
Theresa Wagner, Sergi Torres-Puig, Thatcha Yimthin

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Abstract Extracellular vesicles (EVs) are central components of bacterial secretomes, including the small, cell wall-less Mollicutes. Although EV release in Mollicutes has been reported, proteomic composition and function have not explored yet. We developed a protocol for isolating EVs pathogens Mycoplasma mycoidessubsp. capri (Mmc) (Mycoplasmopsis)bovis examined their functionality. Proteomic analysis demonstrated that mirror proteome homologous strain. exhibited nuclease activity, effectively digesting both circular linear DNA. Notably, elicited immune responses bovine primary blood cells, like those induced by live M. bovis. Our findings reveal can carry plasmids enable horizontal transfer, known as vesiduction. Specifically, natural plasmid pKMK1, with an unknown transmission route, was detected of Mmc 152/93 tetM-containing pIV08 associated released Mmc GM12 strain carrying this plasmid. pIVB08 could be transferred via homo- heterologous vesiduction to Mmc, capricolum subsp. and leachii. Vesiduction impeded membrane disruption but resisted DNase Proteinase K treatment, suggesting protect cargo. These enhance our understanding Mollicutes EVs, particularly host interactions gene transfer.

Язык: Английский

Процитировано

0
Immunogenicity and vaccine efficacy of Actinobacillus pleuropneumoniae -derived extracellular vesicles as a novel vaccine candidate DOI Creative Commons
Su Hyun Park,

Yun Hye Kim,

Hyeon Jin Lee

и другие.

Virulence, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 20, 2025

Actinobacillus pleuropneumoniae (APP) is a significant pathogen in the swine industry, leading to substantial economic losses and highlighting need for effective vaccines. This study evaluates potential of APP-derived extracellular vesicles (APP-EVs) as vaccine candidate compared commercial Coglapix vaccine. APP-EVs, isolated using tangential flow filtration (TFF) cushioned ultracentrifugation, exhibited an average size 105 nm zeta −17.4 mV. These EVs demonstrated stability under external stressors, such pH changes enzymatic exposure were found contain 86 major metabolites. Additionally, APP-EVs induced dendritic cell (DC) maturation Toll-like receptor 4 (TLR4)-dependent manner without cytotoxicity. predominantly elicited Th1-mediated IgG responses immunized mice liver kidney toxicity. Contrarily, unlike Coglapix, which stronger Th2-mediated notable In addition, triggered APP-specific Th1, Th17, cytotoxic T lymphocyte (CTL) promoted activation multifunctional T-cells. Notably, APP-EV immunization enhanced macrophage phagocytosis improved survival rates challenged with APP infection those treated Coglapix. findings suggest that are promising candidates, capable inducing potent T-cell responses, particularly CTL, T-cells, thereby enhancing protective immune response against infection.

Язык: Английский

Процитировано

0
Gut Microbiota‐Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Rats DOI Creative Commons
Macarena Díaz‐Ubilla, Aliosha I. Figueroa‐Valdés, Hugo E. Tobar

и другие.

Journal of Extracellular Vesicles, Год журнала: 2025, Номер 14(3)

Опубликована: Март 1, 2025

ABSTRACT Growing preclinical and clinical evidence suggests a link between gut microbiota dysbiosis problematic alcohol consumption. Extracellular vesicles (EVs) are key mediators involved in bacteria‐to‐host communication. However, their potential role mediating addictive behaviour remains unexplored. This study investigates the of microbiota‐derived bacterial extracellular (bEVs) driving high bEVs were isolated from alcohol‐drinking rat strain (UChB rats), either ethanol‐naïve or following chronic consumption administered intraperitoneally orally to alcohol‐rejecting male female Wistar rats. Both types UChB‐derived increased Wistar's voluntary (three bottle choice test) up 10‐fold ( p < 0.0001), indicating that able sufficient transmit drinking across different strains. Molecular analysis revealed administration did not induce systemic brain inflammation recipient animals, suggesting intake triggered by operates through an inflammation‐independent mechanism. Furthermore, we demonstrate vagus nerve mediates bEV‐induced increase consumption, as bilateral vagotomy completely abolished induced both injected bEVs. Thus, this identifies novel mechanism underlying microbiota‐induced nerve‐dependent manner.

Язык: Английский

Процитировано

0
Opportunities and challenges in harnessing the immunomodulatory potential of bacterial extracellular vesicles as vaccine candidates DOI Creative Commons
Rajendra P. Pawar,

Parul Bardeskar,

Vikrant M. Bhor

и другие.

Discover Immunity., Год журнала: 2025, Номер 2(1)

Опубликована: Апрель 7, 2025

Язык: Английский

Процитировано

0
Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer DOI Creative Commons
Theresa Wagner, Sergi Torres-Puig, Thatcha Yimthin

и другие.

Communications Biology, Год журнала: 2025, Номер 8(1)

Опубликована: Апрель 29, 2025

Язык: Английский

Процитировано

0
Adaptations of Bacterial Extracellular Vesicles in Response to Antibiotic Pressure DOI Open Access
Federica Dell’Annunziata,

Ilaria Cosimato,

Flora Salzano

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5025 - 5025

Опубликована: Май 23, 2025

Extracellular vesicles (EVs) are nanometer-sized lipid structures actively secreted by Gram-negative and Gram-positive bacteria, representing a sophisticated microbial adaptation communication strategy. These involved in biomolecular transport, the regulation of biological processes, modulation host–pathogen interactions, to hostile environmental conditions. EVs also play crucial role virulence, antibiotic resistance, biofilm formation. This review will explore biogenesis, composition, mechanisms outer membrane (OMVs) bacteria (MVs) generated bacteria. In detail, response exposure be addressed. The EV morpho-functional adaptations studied antimicrobial gene determinant spread, survival adverse environments. study aims provide comprehensive overview bacterial physiology, highlighting their ecological, evolutionary, biotechnological implications. An enzymes proteins mainly OMV-mediated resistance provided. insights could open new perspectives for developing therapeutic strategies that counteract secretion applications, such as vaccines drug delivery systems.

Язык: Английский

Процитировано

0
Bacterial Extracellular Vesicles in Oncology: Molecular Mechanisms and Future Clinical Applications DOI Open Access
Piyush Bhanu, Andrew K. Godwin,

Shahid Umar

и другие.

Cancers, Год журнала: 2025, Номер 17(11), С. 1774 - 1774

Опубликована: Май 26, 2025

Bacterial extracellular vesicles (BEVs) have emerged as pivotal mediators of host–microbe interactions, profoundly influencing cancer biology. These nanoscale vesicles, produced by both Gram-positive and Gram-negative bacteria, carry diverse biomolecular cargo such proteins, lipids, nucleic acids, metabolites. BEVs play dualistic roles in tumor promotion suppression modulating the microenvironment, immune responses, genetic regulation. This review synthesizes current understanding various cancers, including gastrointestinal, ovarian, breast, lung, brain, renal malignancies. are highlighted for their potential diagnostic biomarkers, prognostic indicators, therapeutic agents, applications immunotherapy advanced engineering precision medicine. Challenges heterogeneity, standardization, clinical scalability critically analyzed, with case examples providing actionable insights. Future directions emphasize interdisciplinary collaborations, emerging technologies, integration BEV-based tools into workflows. underscores transformative advancing diagnostics therapeutics, paving way innovations oncology.

Язык: Английский

Процитировано

0