Plant-Derived Extracellular Vesicles: Natural Nanocarriers for Biotechnological Drugs
Processes,
Год журнала:
2024,
Номер
12(12), С. 2938 - 2938
Опубликована: Дек. 23, 2024
Plant-derived
extracellular
vesicles
(PDEVs)
are
lipid
bilayer
nanoparticles,
naturally
produced
by
plant
cells,
with
sizes
ranging
from
50
to
500
nm.
Recent
studies
have
highlighted
their
great
potential
in
the
biotechnological
and
medical
fields,
due
natural
origin,
high
biocompatibility
intrinsic
therapeutic
properties.
PDEVs
contain
a
complex
biological
cargo
of
proteins,
lipids,
nucleic
acids
secondary
metabolites,
including
antioxidants
anti-inflammatory
molecules,
making
them
ideal
for
biomedical
applications
such
as
drug
delivery.
These
play
key
role
intercellular
communication
gene
regulation,
proving
be
particularly
promising
personalized
medicine.
ability
improve
stability
bioavailability,
optimizing
targeted
release
minimizing
side
effects.
Despite
some
challenges,
compositional
variability
need
standardized
protocols,
at
gunsight
innovative
research
aimed
improving
loading
capacity
specificity.
This
review
aims
provide
comprehensive
overview
PDEVs,
exploring
structure,
isolation
methods,
functional
characteristics,
applications,
highlighting
advantages
over
synthetic
nanoparticles
animal-derived
vesicles,
leading
an
sustainable
solution
development
new
approaches.
Язык: Английский
Extracellular nanovesicles as neurotherapeutics for central nervous system disorders
Expert Opinion on Drug Delivery,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 7, 2024
Introduction
The
blood-brain
barrier
(BBB)
is
a
highly
selective
structure
that
protects
the
central
nervous
system
(CNS)
while
hindering
delivery
of
many
therapeutic
agents.
This
presents
major
challenge
in
treating
neurological
disorders,
such
as
multiple
sclerosis,
where
effective
drug
to
brain
crucial
for
improving
patient
outcomes.
Innovative
strategies
are
urgently
needed
address
this
limitation.
Язык: Английский
Active herbal ingredients and drug delivery design for tumor therapy: a review
Chinese Journal of Natural Medicines,
Год журнала:
2024,
Номер
22(12), С. 1134 - 1162
Опубликована: Дек. 1, 2024
Язык: Английский
Revolutionizing Medicine: Harnessing Plant-Derived Vesicles for Therapy and Drug Transport
Heliyon,
Год журнала:
2024,
Номер
10(22), С. e40127 - e40127
Опубликована: Ноя. 1, 2024
Язык: Английский
Distribution and Incorporation of Extracellular Vesicles into Chondrocytes and Synoviocytes
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(22), С. 11942 - 11942
Опубликована: Ноя. 6, 2024
Osteoarthritis
(OA)
is
a
chronic
disease
affecting
over
500
million
people
worldwide.
As
the
population
ages
and
obesity
rates
rise,
societal
burden
of
OA
increasing.
Pro-inflammatory
cytokines,
particularly
interleukin-1β,
are
implicated
in
pathogenesis
OA.
Recent
studies
suggest
that
crosstalk
between
cartilage
synovium
contributes
to
development,
but
mechanisms
remain
unclear.
Extracellular
vesicles
(EVs)
were
purified
from
cell
culture-conditioned
medium
via
ultracentrifugation
confirmed
using
transmission
electron
microscopy,
nanoparticle
tracking
analysis,
western
blotting.
We
demonstrated
EVs
taken
up
by
human
synoviocytes
chondrocytes
vitro,
while
vivo
experiments
revealed
fluorescent-labelled
injected
into
mouse
joints
incorporated
synoviocytes.
EV
uptake
was
significantly
inhibited
dynamin-mediated
endocytosis
inhibitors,
indicating
plays
major
role
this
process.
Additionally,
co-culture
with
HEK-293
cells
expressing
red
fluorescent
protein
(RFP)-tagged
CD9
chondrocytic
line
OUMS-27
transfer
RFP-positive
across
600-nm
not
30-nm
filter.
These
findings
released
joint
fluid
within
cartilage,
potentially
facilitating
communication
synovium.
The
results
underscore
importance
pathophysiology.
Язык: Английский
Extracellular Vesicles and PlantCrystals for Improved Bioavailability of Curcumin as a BCS Class IV Drug
Molecules,
Год журнала:
2024,
Номер
29(24), С. 5926 - 5926
Опубликована: Дек. 16, 2024
The
limited
water
solubility
of
active
compounds
remains
a
significant
challenge
for
efficient
dermal
drug
delivery,
particularly
BCS
class
IV
drugs
such
as
curcumin.
This
study
aimed
to
enhance
curcumin’s
penetration
using
two
strategies:
extracellular
vesicles
(EVs)
and
plantCrystals
derived
from
soybeans.
EVs
were
isolated
classical
methods.
However,
containing
(PCEVs)
formed
during
the
preparation
through
bead
milling.
Curcumin
was
either
added
after
PCEVs
formed,
resulting
in
curcumin-added
PCEVs,
or
soybean
dispersion
before
milling,
forming
curcumin-loaded
PCEVs.
formulations
characterized
their
physicochemical
properties
assessed
efficacy
quantitative
dermatokinetic
semi-quantitative
ex
vivo
porcine
ear
models.
results
indicated
that
achieved
higher
compared
EVs,
with
approximately
1.5-fold
2.7-fold
increases
efficacy,
respectively.
Additionally,
showed
superior
depth,
while
curcumin
remained
stratum
corneum.
These
findings
suggest
strategy
via
milling
offers
more
effective
approach
than
improving
topical
delivery
like
Язык: Английский