Extracellular Vesicles Derived from Human Umbilical Mesenchymal Stem Cells Transfected with miR-7704 Improved Damaged Cartilage and Reduced Matrix Metallopeptidase 13
Cells,
Год журнала:
2025,
Номер
14(2), С. 82 - 82
Опубликована: Янв. 9, 2025
We
aimed
to
explore
the
therapeutic
efficacy
of
miR-7704-modified
extracellular
vesicles
(EVs)
derived
from
human
umbilical
cord
mesenchymal
stem
cells
(HUCMSCs)
for
osteoarthritis
(OA)
treatment.
In
vitro
experiments
demonstrated
successful
transfection
miR-7704
into
HUCMSCs
and
isolation
EVs
these
cells.
vivo
used
an
OA
mouse
model
assess
effects
injection
intra-articularly.
Walking
capacity
(rotarod
test),
cartilage
morphology,
histological
scores,
expression
type
II
collagen,
aggrecan,
interleukin-1
beta,
matrix
metalloproteinase
13
(MMP13)
in
were
evaluated.
The
characterized
confirm
their
suitability
use.
IL-1beta-treated
chondrocytes
increased
collagen
decreased
MMP13
after
treatment
with
miR-7704-overexpressed
EVs.
revealed
that
intra-articular
significantly
improved
walking
capacity,
preserved
resulted
higher
scores
compared
controls.
Furthermore,
post
suggests
a
potential
mechanism
observed
effects.
Therefore,
showed
as
innovative
strategy
treating
OA.
Further
investigations
should
focus
on
optimizing
dosage,
understanding
mechanisms,
ensuring
safety
efficacy,
developing
advanced
delivery
systems,
conducting
early-phase
clinical
trials
establish
HUCMSC-derived
management.
Язык: Английский
Bio-3D printing of scaffold-free ADSC-derived cartilage constructs comparable to natural cartilage in vitro
Journal of Orthopaedic Surgery and Research,
Год журнала:
2025,
Номер
20(1)
Опубликована: Фев. 20, 2025
In
end-stage
osteoarthritis
(OA),
osteochondral
defects
reach
the
subchondral
bone
and
cartilage
tissue
of
sufficient
thickness
is
required
to
compensate
for
defects.
Adipose-derived
mesenchymal
stem/stromal
cells
(ADSCs),
which
are
abundant
in
body,
have
potential
differentiate
into
may
be
a
useful
cell
source
regeneration.
If
it
possible
fabricate
ADSC-derived
constructs
that
can
cover
damaged
area,
this
could
lead
development
new
regenerative
therapy
OA
replace
currently
available
treatments.
We
therefore
sought
produce
with
suitable
biological
properties,
similar
native
cartilage,
using
bio-three-dimensional
(3D)
printer.
also
investigated
culture
protocol
ensure
were
fully
mature
even
at
internal
site.
ADSCs
isolated
from
three
rats
expanded
create
spheroids.
The
spheroids
arranged
patches
Kenzan
bio-3D
printer
scaffold-free,
cell-only
constructs.
Basic
fibroblast
growth
factor
(bFGF)
was
added
during
expansion
varying
concentrations
morphogenetic
protein2
(BMP2)
supplemented
chondrogenic
differentiation.
levels
glycosaminoglycans
(GAG)
measured.
histology
compressive
strength
evaluated.
amount
GAG
higher
bFGF
high-BMP2
concentration
groups
than
non-supplemented
low-BMP2
groups.
Chondrocytes
constructs,
extracellular
matrix
homogeneously
positive
safranin
O
staining
type
II
collagen
immunostaining.
consistent
(compressive
4.2
±
1.5
MPa,
n
=
12).
By
optimizing
conditions,
inducing
differentiation,
printing,
we
successfully
fabricated
mechanical
histological
properties
those
articular
cartilage.
Язык: Английский
Advancements in Regenerative Therapies for Orthopedics: A Comprehensive Review of Platelet-Rich Plasma, Mesenchymal Stem Cells, Peptide Therapies, and Biomimetic Applications
Journal of Clinical Medicine,
Год журнала:
2025,
Номер
14(6), С. 2061 - 2061
Опубликована: Март 18, 2025
Background/Objectives:
Regenerative
therapies
have
gained
interest
in
orthopedic
applications
for
their
potential
to
enhance
tissue
regeneration,
functional
recovery,
and
pain
modification.
This
review
evaluates
the
clinical
efficacy
of
platelet-rich
plasma
(PRP),
mesenchymal
stem
cells
(MSCs),
peptide-based
treatments,
biomimetic
materials
care,
with
a
focus
on
reduction
outcomes.
Methods:
A
structured
literature
search
PubMed
(January
2009–January
2025)
identified
160
studies.
After
applying
inclusion
criteria
prioritizing
randomized
controlled
trials
(RCTs)
trials,
59
studies
were
included:
20
PRP,
MSCs,
10
peptide
therapies,
7
biomimetics.
Data
extraction
focused
risk
bias
assessed
using
Cochrane
Risk
Bias
(RoB)
tool
ROBINS-I
tool.
random-effects
meta-regression
analysis
was
conducted
evaluate
impact
therapy
type,
sample
size,
reported
Results:
Meta-regression
MSC
as
most
effective
intervention
(β
=
8.45,
p
<
0.05),
PRP
showing
moderate
improvements,
demonstrating
lowest
effect.
provided
short-term
relief,
particularly
acute
injuries
tendon
repair,
though
inconsistencies
preparation
methods
limited
success
chronic
conditions.
demonstrated
cartilage
regeneration
early
osteoarthritis
improvement,
but
high
costs
ethical
concerns
remain
barriers
widespread
adoption.
Peptide-based
materials,
including
engineered
scaffolds
autologous
protein
solutions,
showed
promise
infection
control
wound
healing,
further
research
is
needed
optimize
dosing,
delivery
methods,
long-term
safety.
Conclusions:
offer
significant
reliable
regenerative
effects,
providing
symptomatic
treatments
emerging
promising
adjuncts.
However,
standardized
protocols
large-scale
are
establish
improve
translation
broader
Язык: Английский
Enhancing the Therapeutic Potential of Human Umbilical Cord Mesenchymal Stem Cells for Osteoarthritis: The Role of Platelet-Rich Plasma and Extracellular Vesicles
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3785 - 3785
Опубликована: Апрель 17, 2025
Osteoarthritis
(OA)
is
a
chronic
degenerative
joint
disease.
Our
previous
study
demonstrated
that
extracellular
vesicles
(EVs)
secreted
by
human
umbilical
cord
mesenchymal
stem
cells
(HUCMSCs),
which
play
crucial
role
in
regenerative
medicine,
have
therapeutic
effects
on
OA.
Additionally,
platelet-rich
plasma
(PRP)
has
been
widely
used
musculoskeletal
diseases
as
it
promotes
wound
healing,
angiogenesis,
and
tissue
remodeling;
however,
its
efficacy
stand-alone
therapy
remains
controversial.
Therefore,
we
investigated
the
of
combining
cell-derived
EVs
with
PRP
an
OA
model.
HUCMSC-derived
treated
were
experimental
group,
whereas
cultured
serum-free
(SF)
or
exosome-depleted
fetal
bovine
serum
(exo(-)FBS)
served
controls.
PRP-treated
HUCMSCs
maintained
their
surface
antigen
characteristics
potential
to
differentiate
into
adipocytes,
osteoblasts,
chondrocytes.
In
model,
mice
+
5%
PRP-derived
showed
significantly
improved
motor
function
compared
controls
comparable
those
+SF
+exo(-)FBS-derived
EVs.
increased
type
II
collagen
aggrecan
decreased
IL-1β
expression
observed
cartilage
transplanted
various
conclusion,
enhances
HUCMSC
differentiation,
treatment
improves
outcomes,
providing
promising
strategy
for
future
clinical
applications.
Язык: Английский