Comparative Analysis of CAZymes from Trichoderma longibrachiatum LMBC 172 Cultured with Three Different Carbon Sources: Sugarcane Bagasse, Tamarind Seeds, and Hemicellulose Simulation

Clean Technologies, Год журнала: 2024, Номер 6(3), С. 994 - 1010

Опубликована: Авг. 8, 2024

The examination of fungal secretomes has garnered attention for its potential to unveil the repertoire secreted proteins, notably CAZymes (Carbohydrate-Active enzymes), across various microorganisms. This study presents findings on categorizing secretome profile by their function and family, derived from filamentous fungus Trichoderma longibrachiatum LMBC 172. cultivation was performed through submerged fermentation with three distinct carbon sources: sugarcane bagasse, tamarind seeds, a control simulating hemicellulose containing 0.5% beechwood xylan plus oat spelt xylan. analysis revealed 206 CAZymes. Each source showed particularities differences. Of these, 89 proteins were produced simultaneously all sources; specifically, 41 using only simulation, 29 when bagasse used as source, 3 seeds used. However, in this last condition, there high intensity xyloglucanase GH74 production, thus reaffirming richness xyloglucan constitution these seeds. When evaluating found two conditions, 18 shown between simulation 11 15 Among found, are representatives different families such glycosyl hydrolases (GHs) that cleave cellulose, hemicellulose, pectin, or other components; carbohydrate esterases (CEs); polysaccharide lyases (PLs); carbohydrate-binding modules (CBMs); auxiliary activity enzymes (AAs). These results demonstrate importance analyzing microorganisms under culture conditions.

Язык: Английский

Thermothelomyces thermophilus cultivated with residues from the fruit pulp industry: enzyme immobilization on ionic supports of a crude cocktail with enhanced production of lichenase

Folia Microbiologica, Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Язык: Английский