Food-inspired peptides from spinach Rubisco endowed with antioxidant, antinociceptive and anti-inflammatory properties

Food Chemistry X, Год журнала: 2023, Номер 18, С. 100640 - 100640

Опубликована: Март 13, 2023

Rubiscolin-6 (amino acid sequence: YPLDLF) is a selective δ-opioid receptor peptide isolated from spinach Rubisco. Its synthetic analogue, YPMDIV the most potent described so far for its increased opioid activity, thus in this work it was considered as lead compound design of twelve new analogues e.g.LMAS1-12. Firstly all novel compounds have been tested their antinociceptive and anti-inflammatory capacity vitro vivo order to evaluate ability maintain or loss original activity. Among them peptides LMAS5-8 gave best results, antioxidant properties investigated along with enzymatic inhibitory ability. Peptide LMAS6 shows strong (154.25 mg TE/g CUPRAC) inhibitor activity on tyrosinase (84.49 KAE/g), indicating potential role food industry anti-browning agent, while LMAS5 LMAS7 possess modest cholinesterase suggesting conceivable use nutraceuticals production.

Язык: Английский

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The potential of plant proteins as antifungal agents for agricultural applications

Synthetic and Systems Biotechnology, Год журнала: 2022, Номер 7(4), С. 1075 - 1083

Опубликована: Июль 16, 2022

Язык: Английский

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Gibberellin-regulated proteins: Emergent allergens

Frontiers in Allergy, Год журнала: 2022, Номер 3

Опубликована: Сен. 9, 2022

About 10 years ago, a protein family was shown for the first time to contain allergenic members, gibberellin-regulated (GRP). The reported member from peach, Pru p 7. One can hypothesize that it not detected before because its physicochemical characteristics overlap with those of lipid transfer (LTP), well-known allergen, or exposure GRP increased due an increase in gibberellin phythormone level plant food, either exogenous endogenous. Like LTPs, GRPs are small cationic proteins disulfide bridges, resistant heat and proteolytic cleavage, involved defense plant. Besides allergens have been described Japanese apricot (Pru m 7), sweet cherry av orange (Cit s pomegranate (Pun g bell pepper (Cap strawberry (Fra GRP), also pollen restriction Cupressaceae tree (Cup 7, Cry j Jun 7). IgE cross-reactivities were between GRPs, peach/cypress citrus/cypress syndromes may therefore be explained these cross-reactivities. clinically relevant, severe adverse reactions sometimes occur association cofactors. More than 60% up 95% sequence identities calculated various three-dimensional models show cleft molecule predict at least three epitopic regions. structure properties matrix effect original source should unraveled understand why, despite ubiquity plants, only few members able sensitize patients.

Язык: Английский

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Plant Defense Peptides: Exploring the Structure–Function Correlation for Potential Applications in Drug Design and Therapeutics

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Defense peptides are part of plants' innate immune system and show widespread occurrence across the plant kingdom. Most them belong to category antimicrobial (AMPs) act as strong chemical weapons against biotic stresses that plants encounter. Plant AMP families exhibit remarkably conserved topology, being cysteine-rich with a number disulfide bridges stabilizing their three-dimensional structure, cationic in nature, having hydrophobic surfaces. However, specific structural properties like amino acid sequence, distribution charged residues, secondary conformations, polar angle play significant roles fine-tuning natural functions interactions biological membranes. Considering vital role defense structure-function relationships, these have been explored for huge potential human health therapeutics. Owing flexibilities, cyclotides considered promising scaffolds drug design frameworks toward various targets applications To elaborate on this idea, we discuss functional heterogeneity within much-conserved cyclotide subfamilies. We Möbius, bracelet, trypsin inhibitor family respect membrane binding affinities pivoting surface charge hydrophobicity. The decrease peptide hydrophobicity increase electrostatic associated more selectivity less toxicity eukaryotic cells, widening range intracellular targets. also application AMPs therapeutics recent advances delivery using nanosystems vehicles.

Язык: Английский

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Plant Peptides Involved in ROS Signalling and Biotic and Abiotic Stress Responses

International Journal of Peptide Research and Therapeutics, Год журнала: 2025, Номер 31(3)

Опубликована: Март 17, 2025

Язык: Английский

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Genomic and functional insights into the diversity of Capsicum annuum defensin gene family

3 Biotech, Год журнала: 2025, Номер 15(4)

Опубликована: Март 24, 2025

Язык: Английский

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Genome-Wide Identification and Bioinformatics Analyses of Host Defense Peptides Snakin/GASA in Mangrove Plants

Genes, Год журнала: 2023, Номер 14(4), С. 923 - 923

Опубликована: Апрель 16, 2023

Host defense peptides (HDPs) are components of plant defensive barriers that resist microbial infection. Members the Snakin/GASA protein family in plants have functions regulating growth, defense, and bacteriostasis. Most mangrove grow coastal zones. In order to survive harsh environments, evolved complex adaptations against microbes. this study, members were identified analyzed genomes three species. Twenty-seven, thirteen, nine candidate found Avicennia marina, Kandelia obovata, Aegiceras corniculatum, respectively. These categorized into subfamilies via phylogenetic analysis. The genes coding for unevenly distributed on chromosomes. Collinearity conservative motif analyses showed K. obovata A. corniculatum underwent multiple gene duplication events. member expression normal leaves infected with pathogenic microorganisms species was verified using real-time quantitative polymerase chain reaction. KoGASA3 4, AcGASA5 10, AmGASA1, 5, 15, 18, 23 increased after This study provides a research basis verification HDPs from suggests directions development utilization marine biological antimicrobial peptides.

Язык: Английский

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Plant Seeds: A Potential Bioresource for Isolation of Nutraceutical and Bioactive Compounds

Methods and protocols in food science, Год журнала: 2024, Номер unknown, С. 333 - 372

Опубликована: Янв. 1, 2024

Язык: Английский

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Chitosan-Based Bioactive Formulations for the Control of Powdery Mildew in Viticulture

Agronomy, Год журнала: 2022, Номер 12(2), С. 495 - 495

Опубликована: Фев. 16, 2022

Grapevine is highly susceptible to fungal diseases, whose incidence and severity increase due climate change. The present work focuses on the assessment of eight combinations natural products with chitosan oligomers fungicidal capacity that may be effective in integrated control powdery mildew, compliance Article 14 European Directive 2009/128/EC. Their efficacy was evaluated field conditions against infections, a plot high disease pressure during growing season (assaying both foliar or root application), overwintering inoculums (chasmothecia) through vitro tests. In addition, their possible biostimulant capacities were based harvest yields. Treatments combination secondary metabolites Streptomyces spp. combined hydrolyzed gluten showed best results terms control. Given these formulations, comparable conventional antifungals, they constitute an interesting alternative for this treatment can, some cases, represent almost half production costs.

Язык: Английский

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On the Applicability of Chitosan Oligomers-Amino Acid Conjugate Complexes as Eco-Friendly Fungicides against Grapevine Trunk Pathogens

Agronomy, Год журнала: 2021, Номер 11(2), С. 324 - 324

Опубликована: Фев. 12, 2021

In a context in which the incidence and severity of grapevine fungal diseases is increasing as result both climate change modern management culture practices, reducing excessive use phytosanitary products viticulture represents major challenge. Specifically, trunk (GTDs), caused by several complexes wood decay or xylem-inhabiting fungi, pose challenge to vineyard sustainability. this study, efficacy chitosan oligomers (COS)–amino acid conjugate against three species belonging Botryosphaeriaceae family (Neofusicoccum parvum, Diplodia seriata, Botryosphaeria dothidea) was investigated vitro planta. tests led EC50 EC90 effective concentrations 254.6−448.5 672.1−1498.5 µg·mL−1 range, respectively, depending on amino involved complex (viz. cysteine, glycine, proline tyrosine) pathogen assayed. A synergistic effect between COS acids observed D. seriata B. dothidea (synergy factors up 2.5 2.8, according Wadley’s method). The formulations based that showed best inhibition rates, COS−tyrosine, were further greenhouse trial grafted vines two varieties (”Tempranillo” 775P “Garnacha” 110R rootstock), artificially inoculated with mentioned species. planta bioassay revealed chosen induced significant decrease disease N. parvum dothidea. summary, reported may be promising enough worthy additional examination larger field trials.

Язык: Английский

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