The First Protocol for In Vitro Propagation of Kalanchoe beharensis Through Adventitious Shoots, a Preliminary Study DOI Creative Commons

Ignacio Moreno-García,

Víctor Rodríguez,

Begoña García‐Sogo

и другие.

Horticulturae, Год журнала: 2024, Номер 10(12), С. 1379 - 1379

Опубликована: Дек. 23, 2024

Kalanchoe beharensis, a vulnerable species according to the International Union for Conservation of Nature, is highly prized its ornamental value and medicinal properties. Therefore, an efficient methodology propagate this ecologically significant would be particular interest. The propagation K. beharensis has traditionally been achieved by seed or cuttings, but these methods are limited in efficiency. Micropropagation provides more controlled alternative enabling vitro production numerous plants small space short period time. Despite advantages, no micropropagation protocol reported literature. In study, we report regeneration beharensis. order implement this, evaluated morphogenetic response leaf root explants media supplemented with auxins, cytokinins, combination both growth regulators. Surprisingly, best results were observed indole-3-acetic acid-supplemented media. Adventitious shoots rooted either hormone-free auxin-supplemented media, acid yielding results. Rooted acclimatized greenhouse, achieving over 80% survival during acclimatization. This improves multiplication rate, utilization, uniformity, providing viable conventional methods.

Язык: Английский

Green Synthesis of Silver Nanoparticles with Extracts from Kalanchoe fedtschenkoi: Characterization and Bioactivities DOI Creative Commons
Jorge L. Mejía-Méndez, Gildardo Sánchez-Ante, Mónica Cerro‐López

и другие.

Biomolecules, Год журнала: 2024, Номер 14(7), С. 782 - 782

Опубликована: Июнь 30, 2024

In this work, the hexane, chloroform, and methanol extracts from

Язык: Английский

Процитировано

8
Exploring the therapeutic mechanism of potential phytocompounds from Kalanchoe pinnata in the treatment of diabetes mellitus by integrating network pharmacology, molecular docking and simulation approach DOI Creative Commons

Rekha Y. Halayal,

Zabin K. Bagewadi, Nayef Abdulaziz Aldabaan

и другие.

Saudi Pharmaceutical Journal, Год журнала: 2024, Номер 32(5), С. 102026 - 102026

Опубликована: Март 13, 2024

Since ancient times, bioactive phytocompounds from different parts of medicinal plants have been used to heal various disease ailments and they are now regarded as a valuable source prevention globally. Kalanchoe pinnata is member the Crassulaceae family; it has long history usage in traditional ayurvedic treatment. Analysis compounds for their potential anti-type-2 diabetes mellitus (T2DM) mechanism along with in-vitro in-silico approaches was studied present research. The alpha-amylase alpha-glucosidase inhibitory activity methanolic extract (α-amylase: IC50 29.50 ± 0.04 μg/ml; α-glucosidase 32.04 0.35 μg/ml) exhibit high degree similarity standard drug acarbose (IC50 35.82 0.14 μg/ml). Different biological databases were list plant, ADME analysis using swissADME carried out screen that obeyed Lipinski rule 5 employed further. STRING KEGG pathway performed gene enrichment followed by network pharmacology identify key target proteins involved DM. AMY2A, NOX4, RPS6KA3, ADRA2A, CHRM5, IL2 identified core targets luteolin, kaempferol, alpha amyrin, stigmasterol modulating neuroactive ligand interaction, P13-AKT, MAPK, PPAR signaling pathways. Molecular docking study binding affinity among K. against aldose reductase, alpha-amylase, alpha-glucosidase, dipeptidyl peptidase IV. Alpha-amylase-friedelin [FRI] alpha-amylase-acarbose [STD] complexes subjected molecular simulation 200 ns duration depicted stability proteins. In current study, employing dual approach enzyme assays yielded comprehensive strong understanding its therapeutic properties, making significant step towards development novel anti-diabetic

Язык: Английский

Процитировано

7
Exploring the Comprehensive Neuroprotective and Anticancer Potential of Afzelin DOI Creative Commons
Mateusz Kciuk, Nitika Garg, Sanchit Dhankhar

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 17(6), С. 701 - 701

Опубликована: Май 28, 2024

Neurodegenerative diseases (Alzheimer’s disease, Parkinson’s Huntington’s and others) cancer, seemingly disparate in their etiology manifestation, exhibit intriguing associations certain cellular molecular processes. Both cancer neurodegenerative involve the deregulation of processes such as apoptosis, proliferation, DNA repair pose a significant global health challenge. Afzelin (kaempferol 3-O-rhamnoside) is flavonoid compound abundant various plant sources. exhibits diverse range biological activities, offering promising prospects for treatment hallmarked by oxidative stress cell death pathways. Its protective potential against also alleviating side effects chemotherapy. This review explores therapeutic implications afzelin, including its capacity to mitigate stress, modulate inflammation, promote regeneration diseases.

Язык: Английский

Процитировано

6
Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts DOI Creative Commons
Jorge L. Mejía-Méndez, Horacio Bach, Ana C. Lorenzo‐Leal

и другие.

Plants, Год журнала: 2023, Номер 12(10), С. 1943 - 1943

Опубликована: Май 10, 2023

In this study, the leaves of Kalanchoe fedtschenkoi were consecutively macerated with hexane, chloroform, and methanol. These extracts used to assess bioactivities plant. The antimicrobial activity was tested against a panel Gram-positive -negative pathogenic bacterial fungal strains using microdilution method. cytotoxicity K. investigated human-derived macrophage THP-1 cells through MTT assay. Finally, anti-inflammatory studied same cell line by measuring secretion IL-10 IL-6. phytoconstituents hexane chloroform evaluated gas chromatography-mass spectrometry (GC/MS). addition, high-performance liquid chromatography (HPLC) study phytochemical content methanol extract. total flavonoid (TFC) extract is also reported. chemical composition Fourier-transform infrared spectroscopy (FTIR). Results revealed that inhibited growth Pseudomonas aeruginosa at 150 μg/mL. At concentration, methicillin-resistant Staphylococcus aureus (MRSA). Regarding their cytotoxicity, three highly cytotoxic IC50 < 3 significantly stimulated 50 μg/mL (p 0.01). GC/MS analyses contain fatty acids, sterols, vitamin E, triterpenes. HPLC analysis demonstrated constituted quercetin kaempferol derivatives. This first report in which profiles are assessed for non-polar polar extracts.

Язык: Английский

Процитировано

11
Pharmacological insights and role of bufalin (bufadienolides) in inflammation modulation: a narrative review DOI
Gagandeep Kaur, Sushma Devi, Akhil Sharma

и другие.

Inflammopharmacology, Год журнала: 2024, Номер 32(5), С. 3057 - 3077

Опубликована: Июль 16, 2024

Язык: Английский

Процитировано

4
Bufadienolide Penetration Through the Skin Membrane and Antiaging Properties of Kalanchoe spp. Juices in Dermal Applications DOI Creative Commons
Anna Hering, Krzysztof Cal, Mariusz Kowalczyk

и другие.

Molecules, Год журнала: 2025, Номер 30(4), С. 802 - 802

Опубликована: Фев. 9, 2025

Skin aging is accelerated by inflammation processes generated oxidative stress and external factors such as UV radiation. Plants belonging to the genus Kalanchoe that are rich sources of antioxidants could potentially strengthen skin barrier if used ingredients in cosmetic formulations. However, their use limited due contents bufadienolides, known cardiotoxins. This study aimed establish a semi-quantitative profile bufadienolides juices K. blossfeldiana, daigremontiana, pinnata using UHPLC combined with charged aerosol detection (CAD) high-resolution mass spectrometry (HR-MS). Additionally, determined ability penetrate Bronaugh Diffusion Cell Apparatus Strat-M membrane. The also assessed ferric molybdenum-reducing powers, well radical scavenging capabilities these plants 2,2-diphenyl-1-picrylhydrazyl (DPPH) 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) methods. vitro antihyaluronidase antityrosinase activities sun protection factor (SPF) were evaluated spectrophotometrically, indicating moderate capability inhibit enzymes, but low SPF for all analyzed juices. semi-qualitative analysis demonstrated presence occurring two from daigremontiana pinnata, highest 1,3,5-bersaldegenin-orthoacetate, bryophyllin-A/bryotoxin-C, bersaldegenin-acetate/bryophyllin-C, diagremontianin. After passing through model, no bufadienolide compounds present subcutaneous filtrate. Antiradical reduction assays revealed antioxidant potential blossfeldiana pinnata. These results indicate have antiaging appear safe dermal applications.

Язык: Английский

Процитировано

0
Impact of Drying Methods on Bioactive Compounds and Antioxidant Properties of Kalanchoe ceratophylla DOI Creative Commons
Lamhot Parulian Manalu, Himawan Adinegoro, Nenie Yustiningsih

и другие.

Scientifica, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Kalanchoe species have been used as herbal medicines in traditional Asian medicine. One of the that has potential a medicinal plant and is still limited its studies ceratophylla . This study investigated effects drying methods, namely, freeze (FD) hot air (HAD), on bioactive compounds, phenolics, flavonoids, antioxidants leaves stems. The content compounds K stems was identified with gas chromatography‐mass spectrometry (GC‐MS) analysis. In addition, antioxidant activity K. also measured. results GC‐MS contain major including gamma.‐Sitosterol, Glutinol, Friedelan‐3‐one, Squalene, Ergost‐5‐en‐3‐ol, (3.β.)‐, Erythritol, Neophytadiene. main component leaf stem extracts gamma‐sitosterol (± 15%), which shows anticancer antidiabetic effects. FD method higher than HAD method. suggests considered appropriate should be to maintain important These findings suggest further research development essential for their pharmacological properties may warranted, developing new drugs.

Язык: Английский

Процитировано

0
Quality Control Strategies for Differentiation of Kalanchoe Species DOI Creative Commons
Evelyn Assis de Andrade, Isadora Machinski, Valter Paes de Almeida

и другие.

Phytochemical Analysis, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

ABSTRACT Kalanchoe species products are commercially available in local markets and by internationally accessible online retailers may exhibit quality issues because of misidentification from similar common names anatomical similarities among related used as feedstock. This study proposes an approach using UPLC‐MS/MS E HPTLC, coupled with morphoanatomical analysis to establish chemical composition pattern data for five species. Subsequently, the methods were validated analyzing commercial purported contain 100% extract. HPTLC profiles demonstrated that quercetin kaempferol derivatives identified primary flavonoids genuine plant extracts. Chemometric showed clear differences no between extracts products. Different patterns anticlinal epidermal cell walls midrib leaves shape arrangement vascular bundles petiole micro‐morphological observed. Evaluation samples revealed labeled containing did not match pharmacobotanical nor These can be considered important tools control derived

Язык: Английский

Процитировано

0
Development of flavonoid-loaded chitosan composite from Kalanchoe pinnata for antibacterial applications DOI Creative Commons
Md Mobarok Karim, Tahera Lasker, Md Ali Zaber Sahin

и другие.

Discover Chemistry., Год журнала: 2025, Номер 2(1)

Опубликована: Март 28, 2025

Язык: Английский

Процитировано

0
Impact of Kalanchoe (Kalanchoe daigremontiana) Supplementation in Goat Maternal Diet on Hepatic and Renal Function and Reproductive Performance DOI Creative Commons
Juan M. Vázquez-García, Gilberto Ballesteros-Rodea, Venancio Cuevas Reyes

и другие.

Biology, Год журнала: 2025, Номер 14(4), С. 376 - 376

Опубликована: Апрель 5, 2025

Kalanchoe daigremontiana, a medicinal plant rich in bioactive compounds, has the potential to serve as feed supplement, reducing reliance on conventional livestock medications while potentially enhancing productivity. This study evaluated effects of K. daigremontiana supplementation over 52 days kidney and liver function, metabolism, weight changes, reproductive efficiency multiparous Alpine goats during breeding season. Fifty-five were assigned either control (CTL, n = 27; 47.6 ± 1.1 kg) or daigremontiana-supplemented (KAL, 28; 1.3 diet. The KAL group received at dry matter-based inclusion rate 2 kg t−1 feed, CTL no supplementation. Blood samples collected four time points assess biochemical markers function. Pregnancy was achieved through natural mating, evaluated. Overall, function did not differ significantly between treatments (p > 0.05). However, associated with increased creatinine < 0.05), ALP 0.001), bilirubin 0.05) specific points, whereas exhibited higher BUN AST albumin total protein ≤ phosphorus 0.01) dates. Other metabolic markers, gain, had mild, transient goat health without impacting Further research is needed explore its long-term optimal dosage for nutrition.

Язык: Английский

Процитировано

0