Revisiting the Multifaceted Roles of Bacteriocins

Microbial Ecology, Год журнала: 2024, Номер 87(1)

Опубликована: Фев. 14, 2024

Abstract Bacteriocins are gene-encoded antimicrobial peptides produced by bacteria. These heterogeneous in terms of structure, activities, biosynthetic clusters, and regulatory mechanisms. widespread nature may contribute to microbial diversity due their capacity target specific Primarily studied as food preservatives therapeutic agents, function natural settings is however less known. This review emphasizes the ecological significance bacteriocins multifunctional exploring bacteriocin distribution, mobility, impact on bacterial population dynamics biofilms.

Язык: Английский

---

Marine Invertebrate Antimicrobial Peptides and Their Potential as Novel Peptide Antibiotics

Marine Drugs, Год журнала: 2023, Номер 21(10), С. 503 - 503

Опубликована: Сен. 23, 2023

Marine invertebrates constantly interact with a wide range of microorganisms in their aquatic environment and possess an effective defense system that has enabled existence for millions years. Their lack acquired immunity sets marine apart from other animals. Invertebrates could rely on innate immunity, providing the first line defense, survival, thriving. The immune includes various biologically active compounds, specifically, antimicrobial peptides. Nowadays, there is revive interest these peptides due to urgent need discover novel drugs against antibiotic-resistant bacterial strains, pressing global concern modern healthcare. Modern technologies offer extensive possibilities development innovative based which can act bacteria, fungi, protozoa, viruses. This review focuses structural peculiarities, biological functions, gene expression, biosynthesis, mechanisms action, regulatory activities, prospects therapeutic use derived invertebrates.

Язык: Английский

---

Antimicrobial Peptides Derived from Bacteria: Classification, Sources, and Mechanism of Action against Multidrug-Resistant Bacteria

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10788 - 10788

Опубликована: Окт. 8, 2024

Antimicrobial peptides (AMPs) are short, usually cationic with an amphiphilic structure, which allows them to easily bind and interact the cellular membranes of viruses, bacteria, fungi, other pathogens. Bacterial AMPs, or bacteriocins, can be produced from Gram-negative Gram-positive bacteria via ribosomal synthesis eliminate competing organisms. AMPs vital in addressing increasing antibiotic resistance various pathogens, potentially serving as alternative ineffective antibiotics. Bacteriocins have a narrow spectrum action, making highly specific antibacterial compounds that target particular bacterial This review covers two main groups bacteriocins by their modes classification, sources positive effects they play on human body, limitations future perspectives

Язык: Английский

---

Lactobacillaceae differentially impact butyrate-producing gut microbiota to drive CNS autoimmunity

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Окт. 27, 2024

Short-chain fatty acids (SCFAs), produced by the gut microbiota, are thought to exert an anti-inflammatory effect on host immune system. The levels of SCFAs and abundance microbiota that produce them depleted in multiple sclerosis (MS), autoimmune disease central nervous system (CNS). mechanisms leading this depletion unknown. Using experimental encephalomyelitis (EAE) as a model for MS, we have previously shown microbiomes divergent their specific commensal

Язык: Английский

---

Toward safer and sustainable food preservation: a comprehensive review of bacteriocins in the food industry

Bioscience Reports, Год журнала: 2025, Номер 45(04), С. 277 - 302

Опубликована: Апрель 1, 2025

Bacteriocins are considered promising natural biopreservatives in the food industry because of their broad spectrum antimicrobial activity against Gram-positive bacteria and foodborne pathogens. This review provides information on several bacteriocins (nisin, pediocin, Micocin®, lacticin 3147, enterocin AS-48), mechanisms action, applications, discussion regulatory requirements for approval as additives by Food Drug Administration (FDA) European Union to improve safety. Nisin (the most studied bacteriocin), recognized generally regarded safe FDA, is used a preservative. Pediocin, derived from Pediococcus acidilactici, shows efficacy Listeria species vegetable meat products. mixture produced Carnobacterium maltaromaticum CB1, effective Clostridium botulinum monocytogenes. Lacticin composed two peptides: Ltnα Ltnβ, synergistic antibacterial with potential applications control pathogens dairy Enterococcin AS-48, Enterococcus faecalis subsp. liquefaciens S-48, exhibits broad-spectrum has been biopreservation number For approval, following criteria must be met: determination identity, chemical composition, safety assessments, recommended concentrations use. Despite difficulties posed large-scale production purification, hold enormous improving shelf life; however, further research required harness future preservation strategies.

Язык: Английский

---

Effects of dietary Nisin on growth performance, immune function, and gut health of broilers challenged by Clostridium perfringens

Journal of Animal Science, Год журнала: 2024, Номер 102

Опубликована: Янв. 1, 2024

Abstract Nisin (Ni) is a polypeptide bacteriocin produced by lactic streptococci (probiotics) that can inhibit the majority of gram-positive bacteria, and improve growth performance broilers, exert antioxidative anti-inflammatory properties. The present study investigated potential preventive effect on necrotic enteritis induced Clostridium perfringens (Cp) challenge. A total 288 Arbor Acres broiler chickens 1-d-olds were allocated using 2 × factorial arrangement into four groups with six replicates (12 per replicate), including: (1) control group (Con, basal diet), (2) Cp challenge (Cp, diet + 1.0 108 CFU/mL Cp), (3) Ni (Ni, 100 mg/kg Ni), (4) (Ni Cp, Cp). results showed decreased average daily gain (ADG) days 15 to 21 (P<0.05) increased interleukin-6 (IL-6) content in serum (P < 0.05), as well significant reduction villus height (VH) ratio VH crypt depth (VCR) increase (CD) jejunum (P<0.05). Furthermore, mRNA expressions Occludin Claudin-1 downregulated (P<0.05), while Caspase3, Caspase9, Bax, Bax/Bcl-2 upregulated jejunum. However, inclusion dietary supplementation significantly improved body weight (BW) 28, ADG CD jejunum, reduced tumor necrosis factor-α (TNF-α) addition levels expression Caspase9 Moreover, altered cecal microbiota composition, which manifested relative abundance phylum Fusobacteriota Shannon index trend Proteobacteria (0.05<P<0.10), Cyanobacteria In conclusion, demonstrated effectively mitigated negative impact challenge-induced damage intestine broilers via reducing inflammatory responses intestinal apoptosis modulating microbial composition.

Язык: Английский

---

Intestinal delivery of encapsulated bacteriocin peptides in cross-linked alginate microcapsules

Food Research International, Год журнала: 2024, Номер 188, С. 114473 - 114473

Опубликована: Май 8, 2024

Oral delivery of larger bioactive peptides (>20 amino acids) to the small intestine remains a challenge due their sensitivity proteolytic degradation and chemical denaturation during gastrointestinal transit. In this study, we investigated capacity crosslinked alginate microcapsules (CLAMs) formed by spray drying protect Plantaricin EF (PlnEF) (C-EF) in gastric conditions dissolve release PlnEF intestine. is an unmodified, two-peptide (PlnE: 33 acids; PlnF: 34 bacteriocin produced Lactiplantibacillus plantarum with antimicrobial gut barrier protective properties. After 2 h incubation simulated fluid (SGF) (pH 1.5), 43.39 % ± 8.27 intact was liberated from CLAMs encapsulates, as determined activity assay. Transfer undissolved fraction intestinal (SIF) 7) for another resulted additional 16.13 4.33 %. No active found SGF or sequential SIF incubations when pepsin (2,000 U/ml) added SGF. To test C-EF contained food matrix, mixed peanut butter (PB) (0.15 g 1.5 PB). A total 12.52 9.09 detected after PB + without pepsin, whereas no included. remaining fractions yielded recovery 46.67 13.09 39.42 11.53 following exposure respectively. Upon accounting incubation, fully protected CLAMs-PB mixture there not significant reduction present. These results show that alone do guard conditions, however, mixing them against proteolysis improved release.

Язык: Английский

---

Current challenges and development strategies of bacteriocins produced by lactic acid bacteria applied in the food industry

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2024, Номер 24(1)

Опубликована: Дек. 14, 2024

Abstract Given the great importance of natural biopreservatives in modern food industry, lactic acid bacteria (LAB)‐producing bacteriocins have gained considerable attention due to their antimicrobial activity against foodborne pathogens and spoilage bacteria. Although numerous LAB‐producing demonstrated efficiency preserving quality various applications, only a limited number these compounds been commercially approved date. The currently unclear gastrointestinal metabolism may pose safety risks, as well cytotoxicity immunogenicity, which need be seriously considered before application. A more noteworthy concern lies whether induce an imbalance gut microbiota, thereby leading alterations abundance health‐associated microorganisms metabolites tract. Accordingly, this review presents unique insights into challenges arising from metabolic interactions between Besides, application industry faces low production yield, weak stability, insufficient activity. corresponding development strategies are proposed for conducting systematic comprehensive evaluation potential risks metabolites. also focus on rational design increase fermentation control enhance hurdle embedding technology improve effects. It definitively discloses perspective become natural, sustainable, safe, eco‐friendly biological preservatives advancement industry.

Язык: Английский

---

Broad-Spectrum Antimicrobial Peptides Suppress Vibrio parahaemolyticus Based on Lactobacillus paracasei A1 Fermentation

Foodborne Pathogens and Disease, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Growing evidence supports the efficacy of antimicrobial peptides against foodborne pathogens, though their spectrum and mechanism can vary depending on origin. We investigated derived from Lactobacillus paracasei A1, effects survival rate bactericidal mechanisms Vibrio parahaemolyticus, identified functional short within them. The crude extracts exhibited antibacterial properties 13 pathogenic bacteria, showing strong inhibition V. parahaemolyticus by disrupting structural integrity cell membranes. At minimum inhibitory concentrations, these significantly disrupted initial adhesion, membrane formation, existing biofilms effectively inhibiting pathogen spread, enhancing agents, reducing food safety risks. LC-MS/MS identification revealed four effective peptides, all demonstrating potent bacteriostatic parahaemolyticus. Our findings indicate that destroy bacterial structures as well stability regeneration biofilms, making them promising candidates for use additives to control pathogens.

Язык: Английский

---

Expanding Layers of Bacteriocin Applications: From Food Preservation to Human Health Interventions

Fermentation, Год журнала: 2025, Номер 11(3), С. 142 - 142

Опубликована: Март 13, 2025

Bacteriocins, ribosomally synthesized by bacteria, have long been recognized for their role in ensuring food safety and security due to antibacterial effects against foodborne pathogens spoilage bacteria. However, recent advancements unveiled expanding potential beyond applications, with increasing evidence of efficacy clinically significant pathogenic biofilm formation, viral infections, even cancer. These emerging discoveries continuously added new layers the application bacteriocins, extending relevance from preservation broader human health interventions. To further harness this potential, various innovative strategies developed overcome traditional limitations associated bacteriocin use. Instead directly employing bacteriocins or bacteriocin-producing bacterial cultures, novel approaches, such as incorporating them into films packaging materials coupling nanoparticles, demonstrated enhanced effectiveness. In review, we examine evolving landscape applications shed light on functional spectrum both health, although some important challenges remain. By analyzing literature technological advancements, highlight how are evolving, opening frontiers use reinforcing significance conventional roles.

Язык: Английский

---