Enhancing the performance of porous silicon biosensors: the interplay of nanostructure design and microfluidic integration
Microsystems & Nanoengineering,
Год журнала:
2024,
Номер
10(1)
Опубликована: Июль 17, 2024
Abstract
This
work
presents
the
development
and
design
of
aptasensor
employing
porous
silicon
(PSi)
Fabry‒Pérot
thin
films
that
are
suitable
for
use
as
optical
transducers
detection
lactoferrin
(LF),
which
is
a
protein
biomarker
secreted
at
elevated
levels
during
gastrointestinal
(GI)
inflammatory
disorders
such
bowel
disease
chronic
pancreatitis.
To
overcome
primary
limitation
associated
with
PSi
biosensors—namely,
their
relatively
poor
sensitivity
due
to
issues
related
complex
mass
transfer
phenomena
reaction
kinetics—we
employed
two
strategic
approaches:
First,
we
sought
optimize
nanostructure
respect
factors
including
layer
thickness,
pore
diameter,
capture
probe
density.
Second,
leveraged
convection
properties
by
integrating
resulting
biosensor
into
3D-printed
microfluidic
system
also
had
one
different
micromixer
architectures
(i.e.,
staggered
herringbone
micromixers
or
microimpellers)
embedded.
We
demonstrated
tailoring
significantly
improved
its
performance,
achieving
limit
(LOD)
50
nM—which
>1
order
magnitude
lower
than
achieved
using
previously-developed
biosensors
this
type.
Moreover,
integration
systems
incorporated
passive
active
further
enhanced
aptasensor’s
sensitivity,
an
additional
reduction
in
LOD
yet
another
magnitude.
These
advancements
demonstrate
potential
combining
PSi-based
technology
create
sensitive
label-free
biosensing
platforms
GI
biomarkers.
Язык: Английский
Evaluation of 3D-Printed Microfluidic Structures for Use in AML-Specific Biomarker Detection of PML::RARA
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(2), С. 497 - 497
Опубликована: Янв. 9, 2025
An
obstacle
for
many
microfluidic
developments
is
the
fabrication
of
its
structures,
which
often
complex,
time-consuming,
and
expensive.
Additive
manufacturing
can
help
to
reduce
these
barriers.
This
study
investigated
whether
results
a
assay
detection
promyelocytic
leukemia
(PML)-retinoic
acid
receptor
α
(RARα)
fusion
protein
(PML::RARA),
thus
differential
diagnosis
acute
(APL),
could
be
transferred
from
borosilicate
glass
structures
additively
manufactured
fluidics.
Digital
light
processing
(DLP)
stereolithography
(SLA)
printers
as
well
different
photopolymerizable
methacrylate-based
resins
were
tested
To
assess
suitability,
both
print
resolution
various
physical
properties,
serializability,
biocompatibility,
functionalization
with
biological
molecules
analyzed.
The
show
that
microfluidics
are
suitable
application
in
diagnostics.
was
demonstrated
by
transferring
sandwich
enzyme-linked
immunosorbent
(ELISA)
PML::RARA
onto
surface
magnetic
microparticles
structure
three-dimensional
(3D)-printed
parts.
A
comparison
conventional
microstructures
suggests
lower
sensitivity
but
highlights
potential
additive
prototyping
microfluidics.
may
contribute
wider
use
biotechnological
or
medical
applications.
Язык: Английский
Enhanced detection of albumin-creatinine ratio in urine using gold nanoparticle-integrated 3D-connector microfluidic paper-based analytical devices for early diagnosis of chronic kidney disease
Microchemical Journal,
Год журнала:
2025,
Номер
unknown, С. 113071 - 113071
Опубликована: Фев. 1, 2025
Язык: Английский
Rapid Microfluidic Biosensor for Point-of-Care Determination of Rheumatoid Arthritis via Anti-Cyclic Citrullinated Peptide Antibody Detection
Biosensors,
Год журнала:
2024,
Номер
14(11), С. 545 - 545
Опубликована: Ноя. 10, 2024
Rheumatoid
arthritis
(RA)
is
a
chronic
autoimmune
disorder
that
causes
extensive
damage
to
multiple
organs
and
tissues
has
no
known
cure.
This
study
introduces
microfluidic
detection
platform
combines
reaction
chip
with
micro-spectrometer
accurately
detect
the
anti-cyclic
citrullinated
peptide
antibody
(anti-CCP
Ab)
biomarker,
commonly
associated
arthritis.
The
surface
of
functionalized
using
streptavidin
enable
subsequent
immobilization
biotinylated-labeled
cyclic
(biotin-CCP)
molecules
through
streptavidin-biotin
reaction.
modified
then
exposed
anti-CCP
Ab,
second
conjugated
horseradish
peroxidase
(HRP)
(2nd
Ab-HRP),
3,3',5,5'-tetramethylbenzidine
(TMB),
stop
solution.
Finally,
concentration
Ab
biomarker
determined
by
analyzing
optical
density
(OD)
colorimetric
product
at
450
nm
micro-spectrometer.
demonstrated
strong
correlation
(R
Язык: Английский
Recent Advances in Polymer Science and Fabrication Processes for Enhanced Microfluidic Applications: An Overview
Micromachines,
Год журнала:
2024,
Номер
15(9), С. 1137 - 1137
Опубликована: Сен. 6, 2024
This
review
explores
significant
advancements
in
polymer
science
and
fabrication
processes
that
have
enhanced
the
performance
broadened
application
scope
of
microfluidic
devices.
Microfluidics,
essential
biotechnology,
medicine,
chemical
engineering,
relies
on
precise
fluid
manipulation
micrometer-sized
channels.
Recent
innovations
materials,
such
as
flexible,
biocompatible,
structurally
robust
polymers,
been
pivotal
developing
advanced
systems.
Techniques
like
replica
molding,
microcontact
printing,
solvent-assisted
injection
3D
printing
are
examined,
highlighting
their
advantages
recent
developments.
Additionally,
discusses
diverse
applications
polymer-based
devices
biomedical
diagnostics,
drug
delivery,
organ-on-chip
models,
environmental
monitoring,
industrial
processes.
paper
also
addresses
future
challenges,
including
enhancing
resistance,
achieving
multifunctionality,
ensuring
biocompatibility,
scaling
up
production.
By
overcoming
these
potential
for
widespread
adoption
impactful
use
technologies
can
be
realized.
Язык: Английский
Microfluidic Immunoarray for Point-of-Care Detection of Cytokines in COVID-19 Patients
ACS Omega,
Год журнала:
2024,
Номер
9(27), С. 29320 - 29330
Опубликована: Июнь 24, 2024
The
"cytokine
storm"
often
induced
in
COVID-19
patients
contributes
to
the
onset
of
"acute
respiratory
distress
syndrome"
(ARDS)
accompanied
by
lung
infection
and
damage,
multiorgan
failure,
even
death.
This
large
increase
pro-inflammatory
cytokines
blood
may
be
related
severity.
Rapid,
on-demand
cytokine
analyses
can
thus
critical
inform
treatment
plans
improve
survival
rates.
Here,
we
report
a
sensitive,
low-cost,
semiautomated
3D-printed
microfluidic
immunoarray
detect
2
CRP
simultaneously
single
10
μL
serum
sample
25
min.
Accuracy
was
validated
analyzing
80
patient
samples,
with
results
well
correlated
commercial
Meso
Scale
protein
immunoassay.
Capture
antibodies
immobilized
detection
microwells
flat
plate-type
flow
chamber
facilitate
immunoassay,
programmable
syringe
pump
automatically
delivering
reagents.
Chemiluminescence
signals
were
captured
dark
box
CCD
camera
integrated
for
30
s.
system
optimized
inflammation
biomarkers
IL-6,
IFN-γ,
serum.
Ultralow
limits
(LODs)
0.79
fg/mL
4.2
CRP,
2.7
IFN-γ
dynamic
ranges
up
100
pg/mL
achieved.
ROC
statistical
showed
relatively
good
diagnostic
value
samples
assigned
WHO
scores
disease
severity,
best
IL-6
CRP.
Monitoring
these
coronavirus
severity
allow
prediction
as
basis
decisions
better
Язык: Английский
Microfluidic-based fluorescence enhancement of silica-embedded carbon dots for direct detection and quantification of unamplified HCV RNA in clinical samples
Analytica Chimica Acta,
Год журнала:
2024,
Номер
1333, С. 343396 - 343396
Опубликована: Ноя. 7, 2024
Hepatitis
C
Virus
(HCV)
is
an
asymptomatic
chronic
infection
with
serious
clinical
consequences.
Timely
and
sensitive
detection
of
HCV
RNA
critical
for
control,
treatment
response
follow
up.
While
current
technologies,
such
as
PCR
or
isothermal
amplification-based
strategies,
are
specific,
they
expensive,
labor
intensive,
time
consuming
limiting
their
use
in
field
small
laboratories.
Язык: Английский
Enhancing porous silicon biosensors performance: the interplay of nanostructure design and microfluidic integration
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 23, 2024
Abstract
In
this
article,
we
investigate
mass
transfer
acceleration
approaches
aimed
at
enhancing
the
performance
of
porous
silicon
(PSi)-based
biosensors.
PSi
biosensors
tend
to
suffer
from
relatively
poor
sensitivity
due
limitations,
which
can
be
attributed
several
factors
including
bulk
diffusion
target
in
solution
toward
biosensor
surface,
hindered
within
layer,
and
simultaneous
reaction
with
immobilized
capture
probe
molecules.
This
study
considers
impact
different
structural
characteristics
(such
as
pore
diameter,
layer
thickness,
density)
on
overall
such
sensors.
Additionally,
look
effect
incorporating
convection
biosensors,
via
their
integration
into
sophisticated
3D-printed
microfluidic
platforms.
The
proposed
designs
include
micromixer
components
that
deployed
for
both
passive
active
mixing
achieve
superior
sensitivity.
We
show
tuning
improve
significantly
–
achieving
a
calculated
limit
detection
(LOD)
50
nM,
is
>
1
order
magnitude
lower
than
achieved
similar
previously
developed
Furthermore,
systems
indeed
aptasensor,
LOD
reduced
by
magnitude.
Язык: Английский