Horticulturae,
Год журнала:
2024,
Номер
10(6), С. 554 - 554
Опубликована: Май 25, 2024
Verticillium
stripe,
caused
by
longisporum,
is
an
emerging
disease
of
canola
(Brassica
napus)
in
Canada.
Studies
were
conducted
to
assess
the
impact
pH
on
both
growth
V.
longisporum
and
its
virulence
host.
Fungal
was
assessed
measuring
colony
diameter
following
14
21
days
incubation
potato
dextrose
agar
at
varying
levels
(4.7,
5.5,
6.5,
7.4,
or
8.6).
The
results
indicated
that
colonies
approximately
16%
greater
7.4
8.6
compared
with
those
5.5.
development
seedling
stage
investigated
using
a
semi-hydroponic
system
different
4.4,
5.4,
6.3,
7.5,
8.4
half-strength
Hoagland’s
solution.
stripe
most
severe
7.5
after
10-day
period
system.
In
second
inoculation
experiment,
seedlings
previously
inoculated
fungus
transplanted
into
potting
mix
amended
four
(5.6,
6.4,
7.2,
7.8).
transplants
cultivated
under
greenhouse
conditions
evaluated
for
severity
plant
maturity.
Disease
greatest
7.8.
This
first
study
effects
canola.
It
suggests
substantial
risk
increased
yield
losses
due
regions
neutral
slightly
alkaline
soils.
Microorganisms,
Год журнала:
2024,
Номер
12(2), С. 251 - 251
Опубликована: Янв. 25, 2024
Clubroot
(Plasmodiophora
brassicae)
is
an
important
soilborne
disease
that
causes
severe
damage
to
cruciferous
crops
in
China.
This
study
aims
compare
the
differences
chemical
properties
and
microbiomes
between
healthy
clubroot-diseased
soils.
To
reveal
difference,
we
measured
soil
microbial
communities
by
sequencing
18S
16S
rRNA
amplicons.
The
available
potassium
diseased
soils
was
higher
than
fungal
diversity
significantly
Ascomycota
Proteobacteria
were
most
dominant
phylum
bacteria
all
samples,
respectively.
Plant-beneficial
microorganisms,
such
as
Chaetomium
Sphingomonas,
more
abundant
Co-occurrence
network
analysis
found
networks
complex
stable
link
number,
density,
clustering
coefficient
of
those
networks.
Our
results
indicate
community
structure
different
from
great
significance
exploring
biological
control
strategies
clubroot
disease.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 24, 2025
Background
and
aims
Clubroot
caused
by
the
soilborne
obligate
parasite
Plasmodiophora
brassicae
,
is
a
devastating
disease
of
Chinese
cabbage
other
crucifers.
The
innate
diversity
adaptability
this
pathogen
pose
significant
challenges
to
effective
control
measures.
However,
varied
response
mechanisms
exhibited
hosts
pathotype
11
at
molecular
level
are
still
unclear.
Methods
results
This
study
investigated
resistance
underlying
mechanism
two
(
Brassica
rapa
)
varieties
(JP
83-1)
P.
through
comparative
transcriptome
analysis
microscopic
study.
Results
demonstrated
that
14
days
after
inoculation
(dai)
critical
time
point
infection
process
for
resistant
variety
inhibit
proliferation
brassica
.
Although
highly
JP
did
not
exhibit
complete
immune
11,
it
against
restricting
its
in
xylem
vessels.
Microscopic
21
dai
revealed
cultivar
(JP)
root
structure
remained
largely
unaffected,
while
roots
susceptible
(83-1)
tissue
distortion
gall
formation,
underscoring
effectiveness
mechanisms.
Comparative
substantial
differences
number
types
differentially
expressed
genes
(DEGs)
between
cultivars,
highlighting
key
pathways
involved
response.
In
(JP),
total
9,433
DEGs
were
identified,
with
4,211
up-regulated
5,222
down-regulated.
contrast,
6,456
DEGs,
2,781
3,675
showed
pronounced
activation
hormone
signaling,
cell
wall,
secondary
metabolism,
redox
state,
signaling
process.
Therefore,
our
speculation
revolves
around
potential
variety,
which
inhibits
via
metabolites,
ROS
also
regulates
physiological
mediated
plant
hormones
such
as
ABA
adapt
adverse
environmental
conditions
water
scarcity
induced
pathogen.
Conclusion
unveils
intricate
defense
potentially
activated
within
when
confronted
offering
valuable
insights
breeding
programs
development
novel
strategies
managing
clubroot
crops.
Furthermore,
highlights
pivotal
role
host-specific
underlie
11.
The Plant Journal,
Год журнала:
2023,
Номер
116(5), С. 1421 - 1440
Опубликована: Авг. 30, 2023
Despite
the
identification
of
clubroot
resistance
genes
in
various
Brassica
crops
our
understanding
genetic
basis
immunity
to
Plasmodiophora
brassicae
infection
model
plant
Arabidopsis
thaliana
remains
limited.
To
address
this
issue,
we
performed
a
screen
142
natural
accessions
and
identified
11
clubroot-resistant
lines.
Genome-wide
association
analysis
several
loci
significantly
linked
with
resistance.
Three
from
two
these
were
targeted
for
deletion
by
CRISPR/Cas9
mutation
resistant
Est-1
Uod-1.
Deletion
Resistance
1
(RPB1)
rendered
both
lines
susceptible
P.
pathotype
P1+.
Further
rpb1
knock-out
Uod-1
showed
that
RPB1
protein
is
required
activation
downstream
defence
responses,
such
as
expression
phytoalexin
biosynthesis
gene
CYP71A13.
has
recently
been
shown
encode
cation
channel
localised
endoplasmic
reticulum.
The
accession
Col-0
lacks
functional
gene;
when
transformed
driven
its
native
promoter
it
capable
activating
transcription
response
infection,
but
not
sufficient
confer
Transient
Nicotiana
tabacum
induced
programmed
cell
death
leaves.
We
conclude
critical
component
Arabidopsis,
acting
pathogen
recognition
elaboration
effective
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(1), С. 785 - 785
Опубликована: Янв. 2, 2023
Clubroot
disease
is
a
soil-borne
caused
by
Plasmodiophora
brassicae.
It
occurs
in
cruciferous
crops
exclusively,
and
causes
serious
damage
to
the
economic
value
of
worldwide.
Although
different
measures
have
been
taken
prevent
spread
clubroot
disease,
most
fundamental
effective
way
explore
use
disease-resistance
genes
breed
resistant
varieties.
However,
resistance
level
plant
hosts
influenced
both
environment
pathogen
race.
In
this
work,
we
described
terms
discovery
current
distribution,
life
cycle,
race
identification
systems;
particular,
summarized
recent
progress
on
control
methods
breeding
practices
for
cultivars.
With
knowledge
these
identified
loci
R
genes,
discussed
feasible
strategies
future.
Journal of Fungi,
Год журнала:
2022,
Номер
8(8), С. 777 - 777
Опубликована: Июль 26, 2022
Clubroot,
a
soil-infective
disease
caused
by
Plasmodiophora
brassicae,
is
serious
affecting
cruciferous
plants
around
the
world.
There
no
effective
control
measure
to
completely
remove
this
pathogen
from
fields
after
infection.
Here,
we
screened
and
identified
two
strains
(Hz36,
Trichoderma
guizhouense;
Hk37,
koningiopsis)
of
gall
clubroot
in
rapeseed
with
biocontrol
potential
for
clubroot.
The
fermentation
broth
Hz36
could
significantly
inhibit
germination
resting
spores
P.
promote
seed
root
growth
rapeseed.
efficiency
strain
on
Arabidopsis
thaliana
was
44.29%
52.18%,
respectively.
qPCR
results
revealed
that
treatment
reduce
content
brassicae
cells,
paraffin
section
analysis
it
delay
development
brassicae.
Strain
Hk37
showed
similar
effects
Hz36,
whose
reach
57.30%
68.01%
A.
thaliana.
These
indicate
have
European Journal of Plant Pathology,
Год журнала:
2023,
Номер
167(4), С. 433 - 451
Опубликована: Июнь 13, 2023
Abstract
The
commercial
aspect
of
growing
Brassica
crops
has
always
been
influenced
by
the
worldwide
occurrence
clubroot
pathogen,
Plasmodiophora
brassicae
.
Clubroot
symptoms
reduce
crop
yield
dramatically
and
resilient
protist
is
hard
to
eradicate
from
infested
soils.
Chemical
treatments
are
not
so
efficient
their
use
allowed
only
in
a
few
areas
world,
none
them
EU.
majority
control
mediated
using
resistant
crops,
but
all
species
have
good
or
durable
resistance
sources
available,
these
can
be
overcome
evolving
new
pathotypes.
Some
commercially
available
biocontrol
agents
tested
found
on
such
as
rapeseed,
cauliflower
Chinese
cabbage
some
extent.
More
organisms
isolated
described
recent
decades
for
many
application
still
long
way
off.
In
this
review
we
summarize
trends
bacterial
fungal
endophytes
well
mechanisms
behind
effects
reported,
antibiosis,
defense
induction
competition
space
nutrients.
There
indeed
plenty
studies
reached
point
where
ready
applied
at
field
scale.
potential
endophytic
microbes
pest
management
against
disease
huge.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 6, 2025
Clubroot
disease
is
an
important
of
cruciferous
crops
worldwide
caused
by
Plasmodiophora
brassicae.
The
pathogen
P.
brassicae
can
infect
almost
all
crops,
resulting
in
a
reduction
yield
and
quality
the
host
plant.
first
part
this
review
outlines
process
infestation,
effectors,
physiological
pathotypes
identification
systems.
latter
highlights
summarizes
various
current
control
measures
research
progress
on
clubroot.
Finally,
we
propose
strategic
concept
for
sustainable
management
In
conclusion,
paper
will
help
to
deepen
knowledge
understanding
integrated
clubroot,
lay
solid
foundation
ABSTRACT
Clubroot,
caused
by
the
biotrophic
protist
Plasmodiophora
brassicae
,
is
a
devastating
root
disease
that
affects
all
members
of
Brassicaceae
family.
Significant
progress
has
been
made
in
understanding
its
occurrence,
life
cycle
and
management
strategies.
This
posed
considerable
threat
to
producers
breeders
globally,
including
South
Asia.
The
reported
India,
Nepal
parts
Bangladesh,
Bhutan,
Pakistan
Sri
Lanka.
cool
moist
climatic
conditions
northern
Himalayan
belt,
combined
with
acidic
soils
inadequate
systems,
are
key
factors,
particularly
areas
India
prone
clubroot
outbreaks.
Disease
strategies
currently
focus
on
soil
amendments,
use
synthetic
fungicides
fumigants
application
resistant
genotypes.
However,
limited
research
conducted
modes
transmission.
Recent
studies
revealed
contaminated
infected
seedlings
major
contributors
spread
pathogen.
Brassica
crops
such
as
B.
juncea
(mustard),
napus
(canola)
oleracea
(cabbage)
vulnerable.
cultivars
like
‘Midas’
‘Big
Sun
111’,
‘Nepa
Star’
‘Kathmandu
Local’,
have
identified
Nepal,
offering
some
potential
for
mitigating
disease.
To
combat
effectively,
there
an
urgent
need
integrated
development
genetic
materials.
These
efforts
should
involve
diverse
stakeholders,
producers,
industries,
government
sectors
academicians,
aiming
advance
challenges
bridging
critical
