Persistence of SARS-CoV-2 and its surrogate, bacteriophage Phi6, on surfaces and in water DOI Creative Commons
Ana K. Pitol,

Samiksha Venkatesan,

Michael Hoptroff

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Март 14, 2023

ABSTRACT The COVID-19 pandemic has motivated research on the persistence of infectious SARS-CoV-2 in environmental reservoirs such as surfaces and water. Viral data been collected for its surrogates, including bacteriophage Phi6. Despite wide use, no side-by-side comparisons between Phi6 exist. Here, we quantified (plastic metal) water evaluated influence that deposition solution viral by using four commonly used solutions: two culture media (DMEM Tryptone Soya Broth (TSB)), Phosphate Buffered Saline (PBS), human saliva. remained significantly longer than SARS-CoV-2, having a half-life 27 hours compared with 15 SARS-CoV-2. viruses was influenced virus solution, but not surface material. when inoculation (DMEM, TSB) Using saliva led to half-lives 9 2 weeks Phi6, 0.5 PBS shorter 4 both all surfaces. Our results showed that, although it frequently surrogate coronaviruses, is an adequate studies quantifying persistence, over-estimates infectiousness. Additionally, our findings reveal need solutions evaluating

Язык: Английский

Examining the stability of viral RNA and DNA in wastewater: Effects of storage time, temperature, and freeze-thaw cycles DOI Creative Commons
Rachel C. Williams, William Bernard Perry, Kathryn Lambert-Slosarska

и другие.

Water Research, Год журнала: 2024, Номер 259, С. 121879 - 121879

Опубликована: Июнь 3, 2024

Wastewater-based epidemiology (WBE) has been demonstrably successful as a relatively unbiased tool for monitoring levels of SARS-CoV-2 virus circulating in communities during the COVID-19 pandemic. Accumulated biobanks wastewater samples allow retrospective exploration spatial and temporal trends public health indicators such chemicals, viruses, antimicrobial resistance genes, possible emergence novel human or zoonotic pathogens. We investigated resilience to time, temperature, freeze-thaw cycles, plus optimal storage conditions maintain stability genetic material (RNA/DNA) viral +ssRNA (Envelope - E, Nucleocapsid N Spike protein S genes SARS-CoV-2), dsRNA (Phi6 phage) circular dsDNA (crAssphage) wastewater. Samples consisted (i) processed extracted samples, (ii) distilled water (iii) raw, unprocessed samples. were stored at -80 °C, -20 4 20 °C 10 days, going through up cycles (once per day). Sample was measured using reverse transcription quantitative PCR, automated electrophoresis, short-read whole genome sequencing. Exploring different areas demonstrated that gene showed greater sensitivity than E genes. Investigating surrogate normalisation viruses Phi6 remains stable comparison laboratory setting crAssphage resilient temperature variation. Recovery raw significantly when which supported by sequencing data all both time negatively impacted metrics. Historical extracts re-quantified 12, 14 16 months after original quantification no major changes. This study highlights importance fast processing extraction following are robust range temperatures.

Язык: Английский

Процитировано

11
Perspectives on phage therapy for health management in aquaculture DOI

Sumeet Rai,

Basmeet Kaur,

Prabjeet Singh

и другие.

Aquaculture International, Год журнала: 2023, Номер 32(2), С. 1349 - 1393

Опубликована: Июль 27, 2023

Язык: Английский

Процитировано

16
PhageDive: the comprehensive strain database of prokaryotic viral diversity DOI Creative Commons
Clara Rolland, Johannes Wittmann, L.C. Reimer

и другие.

Nucleic Acids Research, Год журнала: 2024, Номер 53(D1), С. D819 - D825

Опубликована: Окт. 7, 2024

Abstract Prokaryotic viruses represent the most diverse and abundant biological entities on Earth. So far, data bacteriophages are not standardized, readily available for comparative analyses cannot be linked to rapidly growing (meta)genomic data. We developed PhageDive (https://phagedive.dsmz.de), a comprehensive database prokaryotic gathering all existing dispersed across multiple sources, like scientific publications, specialized databases or internal files of culture collections. allows link own research information through an easy central access, providing fields various experimental (host range, genomic data, etc.) metadata (e.g. geographical origin, isolation source). An important feature is between collection number repository corresponding physical bioresource. To date, covers 1167 phages from three different world-renowned public collections (DSMZ, Félix d’Hérelle Reference Center Bacterial Viruses NCTC) features advanced search function using sections taxonomy morphology by controlled vocabulary ontologies. fully interoperable with other resources including NCBI, Viral Host Range (VHRdb) Institute Pasteur BacDive MediaDive DSMZ.

Язык: Английский

Процитировано

4
Inactivation of the enveloped virus phi6 with hydrodynamic cavitation DOI Creative Commons
Mojca Zupanc, Jure Zevnik, Arijana Filipić

и другие.

Ultrasonics Sonochemistry, Год журнала: 2023, Номер 95, С. 106400 - 106400

Опубликована: Апрель 11, 2023

The COVID -19 pandemic reminded us that we need better contingency plans to prevent the spread of infectious agents and occurrence epidemics or pandemics. Although transmissibility SARS-CoV-2 in water has not been confirmed, there are studies have reported on presence coronaviruses wastewater samples. Since standard treatments designed eliminate viruses, it is utmost importance explore advanced treatment processes can improve help inactivate viruses when needed. This first study investigate effects hydrodynamic cavitation inactivation bacteriophage phi6, an enveloped virus used as a surrogate many studies. In two series experiments with increasing constant sample temperature, reduction up 6.3 logs was achieved. Inactivation phi6 at temperatures 10 20 °C occurs predominantly by mechanical effect results 4.5 logs. At 30 °C, increases 6 logs, where temperature-induced increased susceptibility viral lipid envelope makes more prone inactivation. Furthermore, control without showed temperature alone sufficient cause inactivation, but additional stress still required. RNA degradation confirmed due disrupted bilayer damage. Hydrodynamic cavitation, therefore, potential current potentially emerging pathogenic lower, environmentally relevant temperatures.

Язык: Английский

Процитировано

9
The dual role of tropospheric ozone in controlling COVID-19 outbreaks DOI Creative Commons
Yinon Yecheskel,

Noa Sand,

Lu Zhang

и другие.

Environmental Chemistry Letters, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Язык: Английский

Процитировано

0
Systematic Review and Meta-analysis on the Inactivation Rate of Viruses and Bacteriophage by Solar Wavelength Radiation DOI
Yiding Wang, Gang He,

Fernando Sanchez-Quete

и другие.

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Sunlight is a known biocide, and photodriven inactivation an important avenue for controlling viruses in both natural engineered systems. However, there remain significant unknowns regarding damage to by sunlight, including the impact of wavelength viral characteristics. Herein, systematic review literature meta-analysis was conducted identify rate constants (k-values) when exposed solar wavelengths (280-700 nm) common human surrogates synthetic matrices. We identified 457 k-values, with 356 nonenveloped viruses. Extracted were transformed into UV fluence-normalized k-values isolate most photobiologically relevant spectrum reported first time terms energy, rather than time, based units. Each spectral region assessed independently, UVB illumination reporting highest rates, UVA contributing presence absence photosensitizers, visible light demonstrating no biocidal activity. Inactivation mechanisms are reviewed identifying knowledge gaps translating UVC longer wavelengths. The data compiled this can be applied inform environmental transport viruses, estimate disinfection performance variable conditions, or design systems on light.

Язык: Английский

Процитировано

0
Photodynamic Inactivation of SARS-CoV-2 Infectivity and Antiviral Treatment Effects In Vitro DOI Creative Commons

Svitlana Ziganshyna,

Grit Szczepankiewicz,

Mathias Kuehnert

и другие.

Viruses, Год журнала: 2022, Номер 14(6), С. 1301 - 1301

Опубликована: Июнь 14, 2022

Despite available vaccines, antibodies and antiviral agents, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic still continues to cause disease death. Current treatment options are limited, emerging new mutations a challenge. Thus, novel treatments measures for prevention of viral infections urgently required. Photodynamic inactivation (PDI) is potential by broad variety critical pathogens, including viruses. We explored infectiousness clinical SARS-CoV-2 isolates in Vero cell cultures after PDI-treatment, using photosensitizer Tetrahydroporphyrin-tetratosylate (THPTS) near-infrared light. Replication RNA (qPCR), cytopathic effects (microscopy) mitochondrial activity were assessed. PDI virus suspension with 1 µM THPTS before infection resulted reduction detectable 3 log levels at day 6 similar as previously heat-inactivated virions (<99.9%; p < 0.05). Mitochondrial activity, which was significantly reduced infection, markedly increased uninfected cultures. When applying THPTS-based single had load-reducing effect only higher concentration (3 µM) viability terms PDI-induced toxicity. Repeated 0.3 every 4 h d load more than 99.9% (p 0.05), while maintained. Our data demonstrate that might constitute promising approach SARS-CoV-2. Further testing will if also suitable reduce vivo.

Язык: Английский

Процитировано

13
An Overview of Diverse Strategies To Inactivate Enterobacteriaceae -Targeting Bacteriophages DOI
Sada Raza, Mateusz Wdowiak, Jan Paczesny

и другие.

EcoSal Plus, Год журнала: 2023, Номер 11(1)

Опубликована: Янв. 18, 2023

Bacteriophages are viruses that infect bacteria and thus threaten industrial processes relying on the production executed by bacterial cells. Industries bear huge economic losses due to such recurring resilient infections. Depending specificity of process, there is a need for appropriate methods bacteriophage inactivation, with an emphasis being inexpensive high efficiency. In this review, we summarize reports antiphagents, i.e., antibacteriophage agents inactivation bacteriophages. We focused bacteriophages targeting representatives

Язык: Английский

Процитировано

6
Viricidal Efficacy of a 405‐nm Environmental Decontamination System for Inactivation of Bacteriophage Phi6: Surrogate for SARS‐CoV‐2 DOI Creative Commons
Lucy G. Sinclair,

Zornitsa Ilieva,

Georgina Morris

и другие.

Photochemistry and Photobiology, Год журнала: 2023, Номер 99(6), С. 1493 - 1500

Опубликована: Март 6, 2023

The highly transmittable nature of SARS-CoV-2 has increased the necessity for novel strategies to safely decontaminate public areas. This study investigates efficacy a low irradiance 405-nm light environmental decontamination system inactivation bacteriophage phi6 as surrogate SARS-CoV-2. Bacteriophage was exposed increasing doses (~0.5 mW cm

Язык: Английский

Процитировано

6
Partitioning and inactivation of enveloped and nonenveloped viruses in activated sludge, anaerobic and microalgae-based wastewater treatment systems DOI
Andrés F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martínez-Fraile

и другие.

Water Research, Год журнала: 2023, Номер 248, С. 120834 - 120834

Опубликована: Ноя. 4, 2023

Язык: Английский

Процитировано

6