Semen microbiota are dramatically altered in men with abnormal sperm parameters

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 11, 2024

Abstract There has recently been an explosion of studies implicating the human microbiome in playing a critical role many disease and wellness states. The etiology abnormal semen analysis (SA) parameters is not identified 30% cases; investigations involving may bridge this gap. Here, we explore relationship between alterations sperm parameters. We recruited men presenting for fertility evaluation or vasectomy consultation with proven biological paternity. SA next generation sequencing was performed. Differential abundance testing using Analysis composition Microbiota Bias Correction (ANCOM-BC) performed along canonical correlational microbial community profiling. Men (N = 27) motility showed higher Lactobacillus iners compared to those normal 46) (mean proportion 9.4% versus 2.6%, p 0.046). This persisted on (r 0.392, 0.011). concentration 20) Pseudomonas stutzeri (2.1% 1.0%, 0.024) fluorescens (0.9% 0.7%, 0.010), but lower putida (0.5% 0.8%, 0.020), 53). Major limitations are related study design (cross-sectional, observational). Our results suggest that small group microorganisms play observed perturbations Some these microbes, most notably , have described extensively within other, fertility-related, contexts, whereas others, first report where they potentially implicated. Advances our understanding contribute new therapeutic avenues correcting impairments improving male fertility.

Язык: Английский

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Effects of vaginal microbiota on in vitro fertilization outcomes in women with different infertility causes

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

ABSTRACT Backed by advancements in technologies like microbial sequencing, many studies indicate that the vaginal microbiome is a key marker of female reproductive health. However, further are still needed to investigate correlation between microbiota (VMB) and outcomes assisted technology (ART). Therefore, this study compared VMB two types infertile women undergoing vitro fertilization (IVF) with normal control during implantation window period investigated effects characteristics on IVF outcomes. Vaginal swabs from patients ( n = 85) were collected before embryo transfer (ET), 37) 6–8 days ovulation detection. Results showed differences community structure polycystic ovary syndrome (PCOS) tubal factor infertility (TFI) treatment. The results revealed higher relative abundance Lactobacillus iners non-pregnant group pregnant group. also demonstrated Pseudomonas spp. both groups women. findings suggested composition affects pre-ET high L. may potentially an failure. vagina be adverse for ART pregnancy. IMPORTANCE Many suggest affect fertilization-embryo (IVF-ET) Assessing can optimize timing better (ART) results. This examined distribution using 16S rRNA sequencing. impacted ([less than or equal to] 24 hours). identified as factors post-ET PCOS TFI ovulatory window. These highlight importance considering personalize treatment based type, improving chances success.

Язык: Английский

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The Promising Biological Role of Postbiotics in Treating Human Infertility

Probiotics and Antimicrobial Proteins, Год журнала: 2025, Номер unknown

Опубликована: Янв. 30, 2025

Язык: Английский

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Gynecological Cancers and Microbiota Dynamics: Insights into Pathogenesis and Therapy

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2237 - 2237

Опубликована: Фев. 13, 2024

In recent years, the relationship between microbiota and various aspects of health has become a focal point scientific investigation. Although most studied concern gastrointestinal tract, recently, interest also been extended to other body districts. Female genital tract dysbiosis its possible impact on pathologies such as endometriosis, polycystic ovary syndrome (PCOS), pelvic inflammatory disease (PID), gynecological cancers have unveiled. The incursion pathogenic microbes alters ecological equilibrium vagina, triggering inflammation compromising immune defense, potentially fostering an environment conducive cancer development. common types include cervical, endometrial, ovarian cancer, which occur in women any age but especially postmenopausal women. Several studies highlighted that low presence lactobacilli at vaginal level, consequently, related areas (such endometrium ovary), correlates with higher risk pathology likely contributes increased incidence worse prognosis cancers. complex interplay microbial communities development, progression, treatment gynecologic malignancies is burgeoning field not yet fully understood. intricate crosstalk gut systemic introduces new dimension our understanding objective this review focus attention association provide detailed knowledge for future diagnostic therapeutic strategies.

Язык: Английский

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Editorial: New techniques in microbiome research - volume II: Host-microbiome interactions using ‘meta-omics’ techniques

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 5, 2025

influence human metabolic pathways, offering potential avenues for early diagnosis and targeted therapy.Therapeutic Interventions. Understanding microbiome-disease associations has led to the exploration of microbiome-based therapies. Probiotic prebiotic interventions, as well fecal microbiota transplantation (FMT), are being investigated their restore healthy microbiome composition mitigate disease progression. FMT gained traction a treatment recurrent Clostridioides difficile (C. difficile) infections been explored its role in other conditions such IBD (5).The impact on cancer one most intriguing areas research. Studies suggest that can progression, immune response, even efficacy For example, research shown microbial species residing tumor microenvironment modulate checkpoints affect response immunotherapy. have certain species, Fusobacterium nucleatum, exacerbate colorectal by promoting inflammation genomic instability (6).Microbiome neurological disorders. The gut-brain axis become focal point into neurodegenerative diseases. Meta-omics approaches gut dysbiosis health altering metabolite production, which turn affects neuroinflammation brain function (7). This emerging evidence suggests may play crucial development diseases Parkinson's Alzheimer's, new opportunities therapies.The profoundly impacts susceptibility defense. Dysbiosis body gut, vaginal, skin microbiomes is associated with an increased risk (8). Similarly, compromises function, leading greater gastrointestinal infections, including C. infection (9). Research also highlights gut-lung axis, showing imbalances respiratory suggesting systemic maintaining homeostasis Moreover, sputum severe critically ill influenza patients (10), In addition, indicates alter SARS-CoV-2 virus load COVID-19 severity (11).Microbiome women health. plays critical women's health, particularly context vaginal microbiomes. microbiome, typically dominated Lactobacillus essential environment producing lactic acid acidic pH. Disruptions this balance result bacterial vaginosis, yeast sexually transmitted (STIs) (12). cases vaginosis dysbiosis, increase pathogens like Gardnerella vaginalis Prevotella linked adverse reproductive outcomes, infertility miscarriage (12)(13).In oral changes menstrual cycle. Different profiles were observed during follicular phase, late luteal phases. Alpha diversity beta analyses revealed distinct across four phases (14). lactobacilli used (15). urinary signatures different types diversion (16).Furthermore, significant implications hormonal regulation responses, polycystic ovary syndrome (PCOS) pregnancy outcomes. alterations insulin resistance, imbalances, PCOS, contributing symptoms disturbances (12) (13).The aim Topic offer platform articles expand our understanding host-microbiome interactions using 'meta-omics' techniques. collection includes 5 reviews 14 original articles, highlighting between various These contributions made strides advancing knowledge complex interplay states, cancers (Lin et al. Microbial influences mechanism, prognosis, diagnosis, therapy. A comprehensive review examines intratumoral mechanisms, treatment. It delves roles microbiota, emphasizing responses. Additionally, provides overview recent microbiome-cancer research, summarizing key findings from literature, past current advancements, discussing novel techniques, identifying gaps, outlining future goals. explores cancer, detailed analysis rapidly evolving field (Lin, al., 2025). tract lung investigates communities inhabiting regions system (Cheng, 2024). could mediating oncogenesis through potentially opening therapeutic interventions (Li, 2023). Colorectal (CRC)-associated Porphyromonas endodontalis, Ruminococcus torques, Odoribacter splanchnicus. stage-specific taxa identified, aid diagnosing polyps (BP) CRC stages (Cai Furthermore, specific pathways occurrence series immune-related events (irAEs) gastric, esophageal, colon cancers. Notably, callidus Bacteroides xylanisolvens enriched without irAEs. Several citrulline arginine biosynthesis, irAE (Zhang 2023).The authors reviewed innovative single cell sequencing broad applications addressing questions regarding spatial heterogeneity. advancements deeper insights functional individual cells within (Madhu, etal., microbiota-gut-brain pivotal regulating neuroprotective functions host. Patients (PD) often exhibit (Jia, he appear long-term depression (Li identification classes Negativicutes Selenomonadales direction strategies manage sleep disorders via modulation (Wang, another study, ten (GM) found causal insomnia 2023).Oral During relative abundance Streptococcus genus was significantly higher compared phases, while 7 6 genera exhibited lower phase than notably (Yamazaki 2023).Use probiotic infections. Lactobacilli microenvironment. treating female contribute restoring preserving competing pathogenic bacteria, enhancing local antimicrobial substances (Liu, diversion. studied. Urinary landscapes radical cystectomy (UD) analyzed 2024).Sputum characteristics patients. Bacteroidetes showed depletion cohort. group marked overrepresentation Neisseria, Porphyromonas, Actinobacillus, Alloprevotella, Nanosynbacter lyticus TM7x, Clostridia UCG-014. Alloprevotella inverse correlation cycle threshold (Ct) values. C-reactive protein (CRP) levels demonstrated positive presence Haemophilus (Gu, infection. Distinct patterns identified among individuals, colonized patients, those (CDI), (R-CDI), non-Clostridioides (NOCDI) diarrhea. Potential biomarkers discovered be valuable distinguishing true CDI conditions, improving diagnostic accuracy guiding (Vázquez-Cuesta, 2023).Gut children split-dose bowel preparations. pediatric undergoing PEG preparation colonoscopy, at genus, pathway levels. However, no phylum level (Zou, 2023).Correlations fungal clinical parameters Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) (Niu, One study reveals differences cholesterol pigment gallstone 2024).A combination NaClO H₂O₂ more effective disinfectant, against pathogens, alternative solution efficient control 2022).In conclusion, integration meta-omics techniques revolutionized ability explore microbiome's diseases, providing As these continue evolve, they hold immense promise targets wide range

Язык: Английский

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The Vaginal Virome in Women’s Health and Disease

Microorganisms, Год журнала: 2025, Номер 13(2), С. 431 - 431

Опубликована: Фев. 16, 2025

Most research on the vaginal microbiome has focused bacterial communities (the bacteriome), but viruses, including eukaryotic viruses and bacteriophages, are also important players in health disease states. In this review, we will briefly discuss microbiome, delve into what is known about virome its impact women's health, finish with novel microbial or microbial-derived therapeutics horizon. More studies needed to better prevent treat gynecological, reproductive, neonatal diseases.

Язык: Английский

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Vaginal microbiota and gynecological cancers: a complex and evolving relationship

Infectious Agents and Cancer, Год журнала: 2024, Номер 19(1)

Опубликована: Июнь 14, 2024

Abstract The vagina hosts a community of microorganisms known as the vaginal microbiota. This is relatively stable and straightforward, with Lactobacillus species being most dominant members. microbiota has various functions that are essential for maintaining human health balance. For example, it can metabolise dietary nutrients, produce growth factors, communicate other bacteria, modulate immune system, prevent invasion harmful pathogens. When disrupted, lead to diseases infections. observed disturbance distinguished by reduction in prevalence concurrent rise number bacterial exhibit higher tolerance low oxygen levels. Gynecologic cancers group affect female reproductive organs tissues, such ovaries, uterus, cervix, vagina, vulva, endometrium. These major global problem women. Understanding complex interactions between host may provide new insights into prevention treatment gynecologic cancers. could improve quality life outcomes

Язык: Английский

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Microbiome of pregnancy (literature review)

Vestnik ohrany materinstva i mladenčestva., Год журнала: 2025, Номер 1(3), С. 25 - 41

Опубликована: Янв. 14, 2025

Background. Pregnancy represents a unique immunological and physiological period during which microbial communities can have significant impact on both maternal health birth outcomes. Hormonal, immunological, metabolic changes pregnancy influence the microbiome clinical outcomes, potentially impacting well-being of mother child. Objective: generalization modern scientific concepts about relationship between composition mother’s microbiota its complications, discussion possibility predicting complications using microbiome. Materials methods. An extensive literature review was conducted MEDLINE (PubMed) database as December 2024 with following keywords filter: randomized controlled trial, meta-analysis, systematic review. Research results: Extensive research links dominance certain Lactobacillus strains to different L. cripatus being most favorable strain. Only by existing in normal symbiotic organism is it possible maintain homeostasis. Any shift balance this system leads growth opportunistic flora, turn triggers cascade disorders. GDM, PCOS, premature — these conditions features vaginal microbiocenosis. Conclusion. The female reproductive tract intestine dynamic influenced number factors. More evidence suggests that dysbiosis associated various adverse Knowledge has potential improve accuracy diagnosis, develop targeted interventions, reduce risk perinatal complications.

Язык: Английский

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3D-printed implants targeting gynaecological disease

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 227 - 251

Опубликована: Янв. 1, 2025

Язык: Английский

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Characterization of vaginal microbiomes in clinician-collected bacterial vaginosis diagnosed samples

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

ABSTRACT Bacterial vaginosis (BV) is a type of vaginal inflammation caused by bacterial overgrowth, upsetting the healthy microbiome vagina. Existing clinical testing for BV primarily based upon physical and microscopic examination secretions. Modern PCR-based tests target panels BV-associated microbes, such as Labcorp NuSwab test that targets Atopobium ( Fannyhessea ) vaginae , Megasphaera-1 Vaginosis Associated Bacterium (BVAB)−2 . Remnant clinician-collected swabs underwent DNA extraction 16S V3–V4 rRNA gene sequencing to profile microbes in addition those included test. Community state types (CSTs) were determined using most abundant taxon detected each sample. PCR results panel microbial compared against corresponding profiles. Metabolic pathway abundances characterized via metagenomic prediction from amplicon sequence variants (ASVs). 75 remnant yielded 492 unique ASVs, identifying 83 genera. microbe quantification was strongly concordant with P < 0.01). Samples CST-I (18 18, 100%), CST-II (three three, CST-III (15 17, 88%), CST-V (one one, 100%) largely categorized BV-negative panel, while CST-IV samples (28 36, 78%) BV-positive or BV-indeterminate. predicted metabolic signatures shared across multiple CSTs. These findings highlight robust sequencing-based accurate discrimination CSTs dominated distinct Lactobacilli expanded identification biomarkers. IMPORTANCE poses significant health burden women during reproductive years onward. Current diagnostics rely on either select evaluations technicians. Here, we sequenced profiles previously diagnosed better understand disruptions BV. We show can faithfully reproduce targeted diagnostic improve our knowledge This work highlights robust, agnostic classification scheme potential future development tools.

Язык: Английский

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