Listeria monocytogenes – How This Pathogen Survives in Food-Production Environments?

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Апрель 26, 2022

The foodborne pathogen Listeria monocytogenes is the causative agent of human listeriosis, a severe disease, especially dangerous for elderly, pregnant women, and newborns. Although this infection comparatively rare, it often associated with significant mortality rate 20–30% worldwide. Therefore, microorganism has an important impact on food safety. L. can adapt, survive even grow over wide range production environmental stress conditions such as temperatures, low high pH, salt concentration, ultraviolet lights, presence biocides heavy metals. Furthermore, bacterium also able to form biofilm structures variety surfaces in environments which makes difficult remove allows persist long time. This increases risk contamination facilities finally foods. present review focuses key issues related molecular mechanisms survival adaptation adverse conditions. Knowledge understanding approaches factors will have influence development new, efficient, cost-effective methods control industry, critical ensure

Язык: Английский

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Bacterial Biofilms and Their Implications in Pathogenesis and Food Safety

Foods, Год журнала: 2021, Номер 10(9), С. 2117 - 2117

Опубликована: Сен. 8, 2021

Biofilm formation is an integral part of the microbial life cycle in nature. In food processing environments, bacterial transmissions occur primarily through raw or undercooked foods and by cross-contamination during unsanitary preparation practices. Foodborne pathogens form biofilms as a survival strategy various unfavorable which also become frequent source recurrent contamination outbreaks foodborne illness. Instead focusing on biofilm their pathogenicity individually, this review discusses molecular level how these two physiological processes are connected several common such Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica Escherichia coli. addition, Pseudomonas aeruginosa discussed because it aids persistence many forming polymicrobial contact surfaces, thus significantly elevating safety public health concerns. Furthermore, in-depth analyses molecules with dual functions highlighted.

Язык: Английский

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Multispecies biofilms in fermentation: Biofilm formation, microbial interactions, and communication

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2022, Номер 21(4), С. 3346 - 3375

Опубликована: Июнь 28, 2022

Abstract Food fermentation is driven by microorganisms, which usually coexist as multispecies biofilms. The activities and interactions of functional microorganisms pathogenic bacteria in biofilms have important implications for the quality safety fermented foods. It was verified that biofilm lifestyle benefited fitness harsh environments intensified cooperation competition between members. This review focuses on formation, microbial communication biofilms, application food fermentation. Microbial aggregation adhesion are steps early stage formation. Different biofilm‐forming abilities strategies among lead to several types spatial distribution reflects function. Then, we discuss intrinsic factors external manifestations system succession. Several typical interspecies modes mechanisms were reviewed this review. main limitations studies included relatively small number formed during lack direct evidence formation process within aims provide industry with a sufficient understanding Practical Application : Meanwhile, it offers reference value better controlling utilizing process, improvement yield, quality, products including Chinese Baijiu , cheeese, kefir soy sauce, kombucha, olive.

Язык: Английский

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Cymbopogon citratus Essential Oil: Its Application as an Antimicrobial Agent in Food Preservation

Agronomy, Год журнала: 2022, Номер 12(1), С. 155 - 155

Опубликована: Янв. 8, 2022

Antimicrobial in vitro and situ efficacies of Cymbopogon citratus essential oil (lemongrass, LGEO) against 17 spoilage microorganisms (bacteria, yeasts fungi) were evaluated. Additionally, its chemical composition, antioxidant antibiofilm activities investigated. The LGEO exhibited a strong activity (84.0 ± 0.1%), main constituents citral (61.5%), geraniol (6.6%) 1,8-cineole (6.4%). An antimicrobial evaluation revealed the lowest inhibition zone (1.00 0.00 mm) Pseudomonas fluorescens, highest (18.00 2.46 Candida krusei. values for minimal inhibitory concentration determined to be Salmonella enteritidis C. albicans. Furthermore, ≥250 µL/L suppressed growth Penicillium aurantiogriseum, expansum, chrysogenum italicum. changes molecular structure biofilms produced by fluorescens enteritidis, after their treatment with LGEO, confirmed action on both biofilm-forming bacteria. Moreover, an displayed most effective effectiveness Micrococcus luteus, Serratia marcescens (250 µL/L) expansum (125, 250 500 growing carrot. Our results suggest that as promising natural agent, can applied innovative packaging bakery products different types vegetables, which combines commonly used packing materials addition LGEO.

Язык: Английский

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Listeria monocytogenes: An Inconvenient Hurdle for the Dairy Industry

Dairy, Год журнала: 2023, Номер 4(2), С. 316 - 344

Опубликована: Апрель 19, 2023

Listeria monocytogenes is an opportunistic pathogen that affects specific groups of individuals, with a high mortality rate. The control L. in dairy industries presents particular challenges, as this bacterium capable adhering and forming biofilms, well thriving at refrigerated temperatures, which enables it to persist harsh environments. consumption products has been linked sporadic cases outbreaks listeriosis, frequently detected these retail stores. Moreover, the shown dairy-processing In work, we review main characteristics highlight factors support its persistence processing environments products. We also discuss involved listeriosis since early 1980s, present measures can help prevent occurrence foods food-processing

Язык: Английский

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Genetic engineering of bacteriophages: Key concepts, strategies, and applications

Biotechnology Advances, Год журнала: 2023, Номер 64, С. 108116 - 108116

Опубликована: Фев. 10, 2023

Язык: Английский

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Biofilm formation in food industries: Challenges and control strategies for food safety

Food Research International, Год журнала: 2024, Номер 190, С. 114650 - 114650

Опубликована: Июнь 15, 2024

Язык: Английский

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Integrating Bacteriocins and Biofilm-Degrading Enzymes to Eliminate L. monocytogenes Persistence

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 399 - 399

Опубликована: Янв. 5, 2025

Listeria monocytogenes is a Gram-positive bacterium causing listeriosis, severe infection responsible for significant morbidity and mortality globally. Its persistence on food processing surfaces via biofilm formation presents major challenge, as conventional sanitizers antimicrobials exhibit limited efficacy against biofilm-embedded cells. This study investigates novel approach combining an engineered polysaccharide-degrading enzyme (CAase) with bacteriocin (thermophilin 110) produced by Streptococcus thermophilus. Laboratory assays evaluated the effectiveness of this combination in disrupting biofilms inactivating L. various surfaces. The results demonstrated that CAase effectively disrupts structures, while thermophilin 110 significantly reduces bacterial growth viability. preliminary trials indicate dual-action offers potential alternative to treatments, enhancing safety controlling environments.

Язык: Английский

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Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study

Foods, Год журнала: 2021, Номер 10(7), С. 1484 - 1484

Опубликована: Июнь 26, 2021

Among food-borne pathogens, Listeria monocytogenes continues to pose concerns food business operators due its capacity form biofilm in processing environments. Ozone may be an eco-friendly technology control microbial contaminations, but data concerning effect on are still limited. In this study, the of gaseous ozone at 50 ppm planktonic cells and reference food-related strains was evaluated. caused a reduction loads 3.7 ± 0.4 3.9 Log10 CFU/mL after 10 30 min, respectively. A complete inactivation 6 h treatment observed. Biofilm inhibition eradication treatments (50 ppm, h) resulted significant decrease biomass for 59% tested, whilst slight dampening live cell state conclusion, is not sufficient completely counteract biofilm, it useful as additional tool contrast free-living improve existing sanitization procedures

Язык: Английский

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Susceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cells

Food Microbiology, Год журнала: 2023, Номер 113, С. 104252 - 104252

Опубликована: Март 7, 2023

Plasma-Activated Water (PAW) was generated from tap water using a surface dielectric barrier discharge at different power (26 and 36 W) activation time (5 30 min). The inactivation of three-strain Listeria monocytogenes cocktail in planktonic biofilm state evaluated. PAW W-30 min showed the lowest pH highest hydrogen peroxide, nitrates, nitrites contents effectiveness against cells on state, resulting 4.6 log reductions after 15-min treatment. Although antimicrobial activity biofilms formed stainless steel polystyrene lower, increasing exposure to allowed an >4.5 cycles. mechanisms action were investigated chemical solutions that mimic its physico-chemical characteristics also RNA-seq analysis. main transcriptomic changes affected carbon metabolism, virulence general stress response genes, with several overexpressed genes belonging cobalamin-dependent gene cluster.

Язык: Английский

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