Point-of-care SARS-CoV-2 sensing using lens-free imaging and a deep learning-assisted quantitative agglutination assay DOI
Colin J. Potter, Yanmei Hu, Zhen Xiong

и другие.

Lab on a Chip, Год журнала: 2022, Номер 22(19), С. 3744 - 3754

Опубликована: Янв. 1, 2022

The persistence of the global COVID-19 pandemic caused by SARS-CoV-2 virus has continued to emphasize need for point-of-care (POC) diagnostic tests viral diagnosis. most widely used tests, lateral flow assays in rapid antigen and reverse-transcriptase real-time polymerase chain reaction (RT-PCR), have been instrumental mitigating impact new waves pandemic, but fail provide both sensitive readout patients. Here, we present a portable lens-free imaging system coupled with particle agglutination assay as novel biosensor SARS-CoV-2. This sensor images quantifies individual microbeads undergoing through combination computational deep learning way detect levels complex sample. pseudovirus solution is incubated acetyl cholinesterase 2 (ACE2)-functionalized then loaded into an inexpensive chip. sample imaged in-line holographic microscope image reconstructed from pixel superresolved hologram. Images are analyzed deep-learning algorithm that distinguishes microbead cell debris aggregates, quantified based on network output. We propose procedure using two which results accurate determination concentrations greater than limit detection (LOD) 1.27 × 10

Язык: Английский

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives DOI Creative Commons
Fabio Di Nardo, Matteo Chiarello, Simone Cavalera

и другие.

Sensors, Год журнала: 2021, Номер 21(15), С. 5185 - 5185

Опубликована: Июль 30, 2021

The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform on-site detection target substances. LFIA can be considered as a sort lab-in-a-hand and, together with other point-of-need tests, has represented paradigm shift from sample-to-lab lab-to-sample aiming improve decision making and turnaround time. features LFIAs made them very attractive tool in clinical diagnostic where they patient care enabling more prompt diagnosis treatment decisions. rapidity, simplicity, relative cost-effectiveness, possibility used nonskilled personnel contributed wide acceptance LFIAs. As consequence, molecules, organisms, (bio)markers for purposes, application been rapidly extended fields, including food feed safety, veterinary medicine, environmental control, many others. This review aims provide readers 10-years overview applications, outlining trends main fields compounded annual growth rates. Moreover, future perspectives challenges are discussed.

Язык: Английский

Процитировано

298
Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19 DOI Creative Commons
Wesley Wei‐Wen Hsiao, Trong‐Nghia Le, Dinh Minh Pham

и другие.

Biosensors, Год журнала: 2021, Номер 11(9), С. 295 - 295

Опубликована: Авг. 25, 2021

The development of reliable and robust diagnostic tests is one the most efficient methods to limit spread coronavirus disease 2019 (COVID-19), which caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, require highly trained professional operators. On other hand, lateral flow immunoassay (LFIA) a simpler, cheaper device that can be operated unskilled personnel easily. Unfortunately, current technique has some limitations, mainly inaccuracy in detection. This review article aims highlight recent advances novel technologies detecting SARS-CoV-2 well innovative approaches achieve sensitive specific point-of-care testing. Lastly, we discuss future perspectives on how smartphones Artificial Intelligence (AI) integrated revolutionize detection control surveillance.

Язык: Английский

Процитировано

117
First Description of SARS-CoV-2 Infection in Two Feral American Mink (Neovison vison) Caught in the Wild DOI Creative Commons
Jordi Aguiló-Gisbert, Miguel Padilla‐Blanco, Víctor Lizana

и другие.

Animals, Год журнала: 2021, Номер 11(5), С. 1422 - 1422

Опубликована: Май 16, 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causal agent of COVID-19, is considered a pathogen animal origin that mainly transmitted from human to human. Several species can be naturally or experimentally infected by SARS-CoV-2, with compelling evidence mink highly susceptible SARS-CoV-2 infection. Human-to-mink infection cases have been reported and there are also suggestions mink-to-human occurs. Mink infections date only on fur farms, except for one free- ranging wild near Utah (USA) farm, which suggests transmission pathway farms mink. We now report detection in 13 feral dark brown American (

Язык: Английский

Процитировано

94
Recent Advances of Field-Effect Transistor Technology for Infectious Diseases DOI Creative Commons
Abbas Panahi, Deniz Sadighbayan, Saghi Forouhi

и другие.

Biosensors, Год журнала: 2021, Номер 11(4), С. 103 - 103

Опубликована: Апрель 2, 2021

Field-effect transistor (FET) biosensors have been intensively researched toward label-free biomolecule sensing for different disease screening applications. High sensitivity, incredible miniaturization capability, promising extremely low minimum limit of detection (LoD) at the molecular level, integration with complementary metal oxide semiconductor (CMOS) technology and last but not least operation were amongst predominant motives highlighting these sensors in biosensor community. Although there are various diseases targeted by FET detection, infectious still most demanding sector that needs higher precision realization diagnosis point care (PoC). The COVID-19 pandemic, nevertheless, was an example escalated situation terms worldwide desperate need fast, specific reliable home test PoC devices timely huge numbers people to restrict from further spread. This spawned a wave innovative approaches early antibodies human swab or blood which biosensing gained much more attention due their extraordinary LoD down femtomolar (fM) comparatively faster response time. As novel application especially diseases, this research, we reviewed recent progress on developing accompanied thorough discussion structure Chem/BioFET readout circuitry output signal processing. approach would help engineers biologists gain enough knowledge initiate design accelerated innovations efficient management like COVID-19.

Язык: Английский

Процитировано

64
Nucleic Acid Testing of SARS-CoV-2 DOI Open Access
Hee Min Yoo, Il-Hwan Kim, Seil Kim

и другие.

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(11), С. 6150 - 6150

Опубликована: Июнь 7, 2021

The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. polymerase chain reaction (PCR)-based method including reverse transcription-polymerase (RT-PCR) the most widely used assay for detection of SARS-CoV-2 RNA. Along with RT-PCR method, digital PCR emerged as powerful tool quantify nucleic acid virus high accuracy sensitivity. Non-PCR based techniques such transcription loop-mediated isothermal amplification (RT-LAMP) recombinase (RT-RPA) are considered be simple methods were reviewed in this paper. Non-conventional molecular next-generation sequencing (NGS), CRISPR-based assays nanotechnology improving sensitivity COVID-19 diagnosis. In review, we also focus on standardization testing activity National Metrology Institutes (NMIs) highlight resources reference materials (RM) that provide values specified properties. Finally, summarize useful convenient diagnostics.

Язык: Английский

Процитировано

63
PD-LAMP smartphone detection of SARS-CoV-2 on chip DOI Creative Commons
Ashlee J. Colbert, Dong Hoon Lee, Katherine N. Clayton

и другие.

Analytica Chimica Acta, Год журнала: 2022, Номер 1203, С. 339702 - 339702

Опубликована: Март 9, 2022

Язык: Английский

Процитировано

47
SARS-CoV-2 in wastewater: From detection to evaluation DOI Creative Commons
Danwei Zhang, Samantha Faye Duran Solco, Wei Yang Lim

и другие.

Materials Today Advances, Год журнала: 2022, Номер 13, С. 100211 - 100211

Опубликована: Янв. 25, 2022

SARS-CoV-2 presence in wastewater has been reported several studies and received widespread attention among the Wastewater-based epidemiology (WBE) community. Such can potentially be used as a proxy for early warning of potential COVID-19 outbreak, or mitigation measure virus transmission via contaminated water. In this review, we summarized latest understanding on detection, concentration, evaluation wastewater. Importantly, discuss factors affecting quality surveillance ranging from temperature, pH, starting well chemical pollutants. These greatly affect reliability comparability by various communities across world. Overall, review provides broadly encompassing guidance epidemiological study using surveillance.

Язык: Английский

Процитировано

40
Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives DOI Creative Commons
Tao Dong, Mingyang Wang,

Junchong Liu

и другие.

Chemical Science, Год журнала: 2023, Номер 14(23), С. 6149 - 6206

Опубликована: Янв. 1, 2023

The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has induced public healthcare issues and weakened the global economy significantly. Although SARS-CoV-2 infection is not as fatal initial outbreak, many infected victims suffer from long COVID. Therefore, rapid large-scale testing critical in managing patients alleviating its transmission. Herein, we review recent advances techniques to detect SARS-CoV-2. sensing principles are detailed together with their application domains analytical performances. In addition, advantages limits each method discussed analyzed. Besides molecular diagnostics antigen antibody tests, also neutralizing antibodies emerging variants. Further, characteristics mutational locations different variants epidemiological features summarized. Finally, challenges possible strategies prospected develop new assays meet diagnostic needs. Thus, this comprehensive systematic detection technologies may provide insightful guidance direction for developing tools diagnosis analysis support effective long-term pandemic management control.

Язык: Английский

Процитировано

39
Reviewing methods of deep learning for diagnosing COVID-19, its variants and synergistic medicine combinations DOI Open Access

Qandeel Rafique,

Ali Rehman,

Muhammad Sher Afghan

и другие.

Computers in Biology and Medicine, Год журнала: 2023, Номер 163, С. 107191 - 107191

Опубликована: Июнь 20, 2023

Язык: Английский

Процитировано

25
The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2 DOI Creative Commons
Khaled M. Al-Qaoud, Yusra Obeidat, Tareq Al-Omari

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 7, 2024

Abstract This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining label free electrochemical impedimetric immunosensor with use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized an anti-S antibody, ELISA immunoblot assays confirmed positive response immunized SARS-CoV2 S antigen. developed effective in nasopharyngeal clinical samples from suspected cases. key advantage this biosensor its remarkable sensitivity, capability very low concentrations target analyte, detection limit 5.65 pg/mL. attribute makes it highly suitable practical point-of-care (POC) applications, particularly analyte count scenarios, without requiring amplification. Furthermore, has wide dynamic range detection, spanning 11.56 to 740 ng/mL, which applicable sample analysis typical setting.

Язык: Английский

Процитировано

13