Point-of-care SARS-CoV-2 sensing using lens-free imaging and a deep learning-assisted quantitative agglutination assay DOI
Colin J. Potter, Yanmei Hu, Zhen Xiong

et al.

Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(19), P. 3744 - 3754

Published: Jan. 1, 2022

The persistence of the global COVID-19 pandemic caused by SARS-CoV-2 virus has continued to emphasize need for point-of-care (POC) diagnostic tests viral diagnosis. most widely used tests, lateral flow assays in rapid antigen and reverse-transcriptase real-time polymerase chain reaction (RT-PCR), have been instrumental mitigating impact new waves pandemic, but fail provide both sensitive readout patients. Here, we present a portable lens-free imaging system coupled with particle agglutination assay as novel biosensor SARS-CoV-2. This sensor images quantifies individual microbeads undergoing through combination computational deep learning way detect levels complex sample. pseudovirus solution is incubated acetyl cholinesterase 2 (ACE2)-functionalized then loaded into an inexpensive chip. sample imaged in-line holographic microscope image reconstructed from pixel superresolved hologram. Images are analyzed deep-learning algorithm that distinguishes microbead cell debris aggregates, quantified based on network output. We propose procedure using two which results accurate determination concentrations greater than limit detection (LOD) 1.27 × 10

Language: Английский

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives DOI Creative Commons
Fabio Di Nardo, Matteo Chiarello, Simone Cavalera

et al.

Sensors, Journal Year: 2021, Volume and Issue: 21(15), P. 5185 - 5185

Published: July 30, 2021

The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform on-site detection target substances. LFIA can be considered as a sort lab-in-a-hand and, together with other point-of-need tests, has represented paradigm shift from sample-to-lab lab-to-sample aiming improve decision making and turnaround time. features LFIAs made them very attractive tool in clinical diagnostic where they patient care enabling more prompt diagnosis treatment decisions. rapidity, simplicity, relative cost-effectiveness, possibility used nonskilled personnel contributed wide acceptance LFIAs. As consequence, molecules, organisms, (bio)markers for purposes, application been rapidly extended fields, including food feed safety, veterinary medicine, environmental control, many others. This review aims provide readers 10-years overview applications, outlining trends main fields compounded annual growth rates. Moreover, future perspectives challenges are discussed.

Language: Английский

Citations

297
Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19 DOI Creative Commons
Wesley Wei‐Wen Hsiao, Trong‐Nghia Le, Dinh Minh Pham

et al.

Biosensors, Journal Year: 2021, Volume and Issue: 11(9), P. 295 - 295

Published: Aug. 25, 2021

The development of reliable and robust diagnostic tests is one the most efficient methods to limit spread coronavirus disease 2019 (COVID-19), which caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, require highly trained professional operators. On other hand, lateral flow immunoassay (LFIA) a simpler, cheaper device that can be operated unskilled personnel easily. Unfortunately, current technique has some limitations, mainly inaccuracy in detection. This review article aims highlight recent advances novel technologies detecting SARS-CoV-2 well innovative approaches achieve sensitive specific point-of-care testing. Lastly, we discuss future perspectives on how smartphones Artificial Intelligence (AI) integrated revolutionize detection control surveillance.

Language: Английский

Citations

116
First Description of SARS-CoV-2 Infection in Two Feral American Mink (Neovison vison) Caught in the Wild DOI Creative Commons
Jordi Aguiló-Gisbert, Miguel Padilla‐Blanco, Víctor Lizana

et al.

Animals, Journal Year: 2021, Volume and Issue: 11(5), P. 1422 - 1422

Published: May 16, 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causal agent of COVID-19, is considered a pathogen animal origin that mainly transmitted from human to human. Several species can be naturally or experimentally infected by SARS-CoV-2, with compelling evidence mink highly susceptible SARS-CoV-2 infection. Human-to-mink infection cases have been reported and there are also suggestions mink-to-human occurs. Mink infections date only on fur farms, except for one free- ranging wild near Utah (USA) farm, which suggests transmission pathway farms mink. We now report detection in 13 feral dark brown American (

Language: Английский

Citations

94
Recent Advances of Field-Effect Transistor Technology for Infectious Diseases DOI Creative Commons
Abbas Panahi, Deniz Sadighbayan, Saghi Forouhi

et al.

Biosensors, Journal Year: 2021, Volume and Issue: 11(4), P. 103 - 103

Published: April 2, 2021

Field-effect transistor (FET) biosensors have been intensively researched toward label-free biomolecule sensing for different disease screening applications. High sensitivity, incredible miniaturization capability, promising extremely low minimum limit of detection (LoD) at the molecular level, integration with complementary metal oxide semiconductor (CMOS) technology and last but not least operation were amongst predominant motives highlighting these sensors in biosensor community. Although there are various diseases targeted by FET detection, infectious still most demanding sector that needs higher precision realization diagnosis point care (PoC). The COVID-19 pandemic, nevertheless, was an example escalated situation terms worldwide desperate need fast, specific reliable home test PoC devices timely huge numbers people to restrict from further spread. This spawned a wave innovative approaches early antibodies human swab or blood which biosensing gained much more attention due their extraordinary LoD down femtomolar (fM) comparatively faster response time. As novel application especially diseases, this research, we reviewed recent progress on developing accompanied thorough discussion structure Chem/BioFET readout circuitry output signal processing. approach would help engineers biologists gain enough knowledge initiate design accelerated innovations efficient management like COVID-19.

Language: Английский

Citations

63
Nucleic Acid Testing of SARS-CoV-2 DOI Open Access
Hee Min Yoo, Il-Hwan Kim, Seil Kim

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(11), P. 6150 - 6150

Published: June 7, 2021

The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. polymerase chain reaction (PCR)-based method including reverse transcription-polymerase (RT-PCR) the most widely used assay for detection of SARS-CoV-2 RNA. Along with RT-PCR method, digital PCR emerged as powerful tool quantify nucleic acid virus high accuracy sensitivity. Non-PCR based techniques such transcription loop-mediated isothermal amplification (RT-LAMP) recombinase (RT-RPA) are considered be simple methods were reviewed in this paper. Non-conventional molecular next-generation sequencing (NGS), CRISPR-based assays nanotechnology improving sensitivity COVID-19 diagnosis. In review, we also focus on standardization testing activity National Metrology Institutes (NMIs) highlight resources reference materials (RM) that provide values specified properties. Finally, summarize useful convenient diagnostics.

Language: Английский

Citations

63
PD-LAMP smartphone detection of SARS-CoV-2 on chip DOI Creative Commons
Ashlee J. Colbert, Dong Hoon Lee, Katherine N. Clayton

et al.

Analytica Chimica Acta, Journal Year: 2022, Volume and Issue: 1203, P. 339702 - 339702

Published: March 9, 2022

Language: Английский

Citations

46
Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives DOI Creative Commons
Tao Dong, Mingyang Wang,

Junchong Liu

et al.

Chemical Science, Journal Year: 2023, Volume and Issue: 14(23), P. 6149 - 6206

Published: Jan. 1, 2023

The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has induced public healthcare issues and weakened the global economy significantly. Although SARS-CoV-2 infection is not as fatal initial outbreak, many infected victims suffer from long COVID. Therefore, rapid large-scale testing critical in managing patients alleviating its transmission. Herein, we review recent advances techniques to detect SARS-CoV-2. sensing principles are detailed together with their application domains analytical performances. In addition, advantages limits each method discussed analyzed. Besides molecular diagnostics antigen antibody tests, also neutralizing antibodies emerging variants. Further, characteristics mutational locations different variants epidemiological features summarized. Finally, challenges possible strategies prospected develop new assays meet diagnostic needs. Thus, this comprehensive systematic detection technologies may provide insightful guidance direction for developing tools diagnosis analysis support effective long-term pandemic management control.

Language: Английский

Citations

38
Reviewing methods of deep learning for diagnosing COVID-19, its variants and synergistic medicine combinations DOI Open Access

Qandeel Rafique,

Ali Rehman,

Muhammad Sher Afghan

et al.

Computers in Biology and Medicine, Journal Year: 2023, Volume and Issue: 163, P. 107191 - 107191

Published: June 20, 2023

Language: Английский

Citations

25
The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2 DOI Creative Commons
Khaled M. Al-Qaoud, Yusra Obeidat, Tareq Al-Omari

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 7, 2024

Abstract This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining label free electrochemical impedimetric immunosensor with use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized an anti-S antibody, ELISA immunoblot assays confirmed positive response immunized SARS-CoV2 S antigen. developed effective in nasopharyngeal clinical samples from suspected cases. key advantage this biosensor its remarkable sensitivity, capability very low concentrations target analyte, detection limit 5.65 pg/mL. attribute makes it highly suitable practical point-of-care (POC) applications, particularly analyte count scenarios, without requiring amplification. Furthermore, has wide dynamic range detection, spanning 11.56 to 740 ng/mL, which applicable sample analysis typical setting.

Language: Английский

Citations

12
Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications DOI Open Access

Keith J. M. Moore,

Jeremy Cahill,

Guy Aidelberg

et al.

Journal of Biomolecular Techniques JBT, Journal Year: 2021, Volume and Issue: 32(3), P. 228 - 275

Published: Sept. 1, 2021

As the second year of COVID-19 pandemic begins, it remains clear that a massive increase in ability to test for SARS-CoV-2 infections myriad settings is critical controlling and preparing future outbreaks. The current gold standard molecular diagnostics polymerase chain reaction (PCR), but extraordinary unmet demand testing variety environments means both complementary supplementary solutions are still needed. This review highlights role loop-mediated isothermal amplification (LAMP) has had filling this global need, providing faster easier testing, what can do applications, pathogens, preparation describes state art research LAMP-based as well its implications other pathogens testing. authors represent LAMP (gLAMP) Consortium, an international collective, which regularly met share their experiences on deployment best practices; sections devoted all aspects including preanalytic sample processing, target amplification, amplicon detection, then hardware software required discussed, finally, summary regulatory landscape provided. Included series first-person accounts method development deployment. final discussion section provides reader with distillation most validated methods paths implementation. also aims provide practical information insight range audiences: audience, help accelerate through sharing implementation get up running quickly; public health, clinical, policy convey breadth effect have offer.

Language: Английский

Citations

43