Biofilm Lifestyle in Recurrent Urinary Tract Infections

Life, Год журнала: 2023, Номер 13(1), С. 148 - 148

Опубликована: Янв. 4, 2023

Urinary tract infections (UTIs) represent one of the most common that are frequently encountered in health care facilities. One main mechanisms used by bacteria allows them to survive hostile environments is biofilm formation. Biofilms closed bacterial communities offer protection and safe hiding, allowing evade host defenses hide from reach antibiotics. Inside communities, show an increased rate horizontal gene transfer exchange resistance virulence genes. Additionally, communication within orchestrate expression genes, which further cements infestation increases invasiveness infection. These facts stress necessity continuously updating our information understanding etiology, pathogenesis, eradication methods this growing public concern. This review seeks understand role formation recurrent urinary tact outlining underlying different uropathogens, addition shedding light on some strategies.

Язык: Английский

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Design, synthesis, in-vitro biological profiling and molecular docking of some novel oxazolones and imidazolones exhibiting good inhibitory potential against acetylcholine esterase

Journal of Biomolecular Structure and Dynamics, Год журнала: 2024, Номер unknown, С. 1 - 18

Опубликована: Фев. 13, 2024

Heterocyclic compounds with oxazole and imidazole rings in their structure have disclosed momentous biological aptitudes. Taking into account superlative attributes, the present study was designed to introduce a new synthetic scheme make derivatives tremendous futuristic pharmacological potentialities. Series of Oxazolones were synthesized by using substituted benzaldehyde benzyl halides produce respective 1 (a–d) which further reacted hippuric acid yield oxazolones 2 (a–e). Newly then 4-chloroaniline corresponding imidazolones 3 All characterized FTIR NMR spectroscopic techniques. Docking studies Compounds conducted AutoDock Vina analyzed PYMOL. oxazolone imidazolone exhibited antioxidant potential, demonstrated IC50 values compared ascorbic standard. Oxazolone (2a–2e) good acetyl cholinesterase inhibitory potential whereas Imidazolone series did not show significant inhibition as shown donepezil all against acetylcholinesterase favorable binding affinity, indicating for in-vivo studies. It is notable that novel both maximum percentage 75.9 (IC50 12.9 ± 0.0573 µM/mL) compound 2d while 2a showed AChE %age 75.49 7.8 0.0218 µM/mL).

Язык: Английский

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Anti-Quorum Sensing Activities of Gliptins against Pseudomonas aeruginosa and Staphylococcus aureus

Biomedicines, Год журнала: 2022, Номер 10(5), С. 1169 - 1169

Опубликована: Май 18, 2022

The development of bacterial resistance to traditional antibiotics constitutes an emerging public health issue. Promising approaches have been innovated conquer resistance, and targeting virulence is one these approaches. Bacterial mitigation offers several merits, as antivirulence agents do not affect the growth bacteria hence induce develop resistance. In this direction, numerous drugs repurposed prior their clinical use alone or in combination with antibiotics. Quorum sensing (QS) plays a key role controlling virulence. current study, dipeptidase inhibitor-4 (DPI-4) antidiabetic gliptins were screened for anti-quorum (anti-QS) activities against Gram-negative Pseudomonas aeruginosa Gram-positive Staphylococcus aureus. Upon assessing antibiofilm activities, ten tested significantly diminished biofilm formation. particular, sitagliptin exhibited most efficient activity, so it was chosen representative all further investigate its activity. Sitagliptin protected mice from P. S. aureus pathogenesis. Furthermore, downregulated QS-encoding genes To test anti-QS gliptins, detailed molecular docking study conducted evaluate gliptins' binding affinities QS receptors, which helped explain particularly omarigliptin. conclusion, evaluates possible that could be promising novel candidates treatment aggressive -positive infections either adjuvants other

Язык: Английский

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Terazosin Interferes with Quorum Sensing and Type Three Secretion System and Diminishes the Bacterial Espionage to Mitigate the Salmonella Typhimurium Pathogenesis

Antibiotics, Год журнала: 2022, Номер 11(4), С. 465 - 465

Опубликована: Март 30, 2022

Salmonella enterica is an invasive intracellular pathogen and hires diverse systems to manipulate its survival in the host cells. could eavesdrop on cells, sensing responding produced adrenergic hormones other neurotransmitters, which results augmentation of virulence establishes accommodation The current study aims assess anti-virulence effect α-adrenergic antagonist terazosin S. Typhimurium. Our findings show that significantly reduced Typhimurium adhesion biofilm formation. Furthermore, decreased invasion replication Interestingly, vivo, protected mice from pathogenesis. To understand activity, quorum (QS), bacterial espionage, type three secretion system (T3SS) was studied. Strikingly, competed membranal sensors sense down-regulated their encoding genes, indicates ability diminish eavesdropping Moreover, Chromobacterium violaceum QS-controlled pigment production interfered with QS receptor Lux-homolog SdiA, possible terazosin-mediated anti-QS activity. expression T3SS genes. In conclusion, may mitigate owing hindering down-regulating genes besides inhibition espionage.

Язык: Английский

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Sodium Citrate Alleviates Virulence in Pseudomonas aeruginosa

Microorganisms, Год журнала: 2022, Номер 10(5), С. 1046 - 1046

Опубликована: Май 18, 2022

The development of bacterial resistance is an insistent global health care issue, especially in light the dwindled supply new antimicrobial agents. This mandates innovative approaches to overcome obstacle. Mitigation virulence interesting approach that offers multiple advantages. Employing safe chemicals or drugs mitigate additive advantage. In current study, vitro antivirulence activities citrate were evaluated. Significantly, sodium inhibited biofilm formation at sub-MIC concentrations. Furthermore, decreased production factors protease and pyocyanin diminished motility. Quorum sensing (QS) communicative system cells utilize communicate with each other regulate host cells. present silico blocked Pseudomonas QS receptors downregulated expression QS-encoding genes. conclusion, showed a significant ability diminish interfered QS; it could serve as adjuvant traditional antibiotic treatment for aggressive resistant infections such aeruginosa infections.

Язык: Английский

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Characterization of the Anti-Biofilm and Anti-Quorum Sensing Activities of the β-Adrenoreceptor Antagonist Atenolol against Gram-Negative Bacterial Pathogens

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(21), С. 13088 - 13088

Опубликована: Окт. 28, 2022

The development of bacterial resistance to antibiotics is an increasing public health issue that worsens with the formation biofilms. Quorum sensing (QS) orchestrates virulence and controls biofilm. Targeting promising approach overcome increment antibiotics. In a previous detailed in silico study, anti-QS activities twenty-two β-adrenoreceptor blockers were screened supposing atenolol as candidate. current study aims evaluate anti-QS, anti-biofilm anti-virulence blocker against Gram-negative bacteria Serratia marcescens, Pseudomonas aeruginosa, Proteus mirabilis. An was conducted binding affinity S. marcescens SmaR QS receptor, P. aeruginosa QscR mirabilis MrpH adhesin. activity evaluated tested strains vitro vivo. present finding shows considerable ability compete proteins significantly downregulated expression QS- virulence-encoding genes. Atenolol showed significant reduction biofilm formation, enzyme production, motility. Furthermore, diminished capacity for killing protected mice. conclusion, has potential can be used adjuvant treatment aggressive infections.

Язык: Английский

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Diminishing the Pathogenesis of the Food-Borne Pathogen Serratia marcescens by Low Doses of Sodium Citrate

Biology, Год журнала: 2023, Номер 12(4), С. 504 - 504

Опубликована: Март 26, 2023

Protecting food from bacterial contamination is crucial for ensuring its safety and avoiding foodborne illness. Serratia marcescens one of the contaminants that can form biofilms pigments spoil product could cause infections illness to consumer. Food preservation essential diminish such or at least reduce their pathogenesis; however, it should not affect odor, taste, consistency must be safe. Sodium citrate a well-known safe additive current study aims evaluate anti-virulence anti-biofilm activity low concentrations against S. marcescens. The antibiofilm activities sodium were evaluated phenotypically genotypically. results showed significant effect on decreasing biofilm formation other virulence factors, as motility production prodigiosin, protease, hemolysins. This owed downregulating virulence-encoding genes. An in vivo investigation was conducted mice histopathological examination isolated tissues liver kidney confirmed citrate. In addition, an silico docking binding ability quorum sensing (QS) receptors regulates virulence. marked virtual compete QS proteins, which explain citrate's effect. conclusion, used prevent by bacteria.

Язык: Английский

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Drug repositioning: doxazosin attenuates the virulence factors and biofilm formation in Gram-negative bacteria

Applied Microbiology and Biotechnology, Год журнала: 2023, Номер 107(11), С. 3763 - 3778

Опубликована: Апрель 20, 2023

Язык: Английский

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What’s old is new again: Insights into diabetic foot microbiome

World Journal of Diabetes, Год журнала: 2023, Номер 14(6), С. 680 - 704

Опубликована: Июнь 14, 2023

Diabetes is a chronic disease that considered one of the most stubborn global health problems continues to defy efforts scientists and physicians. The prevalence diabetes in population grow alarming levels year after year, causing an increase incidence complications care costs all over world. One major complication high susceptibility infections especially lower limbs due immunocompromised state diabetic patients, which definitive factor cases. Diabetic foot continue be common patients are associated with risk serious such as bone infection, limb amputations, life-threatening systemic infections. In this review, we discussed circumstances infection well some commonly isolated pathogens from related virulence behavior. addition, shed light on different treatment strategies aim at eradicating infection.

Язык: Английский

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Synergistic Benefits: Exploring the Anti-Virulence Effects of Metformin/Vildagliptin Antidiabetic Combination against Pseudomonas aeruginosa via Controlling Quorum Sensing Systems

Biomedicines, Год журнала: 2023, Номер 11(5), С. 1442 - 1442

Опубликована: Май 14, 2023

The repurposing of drugs is one the most competent strategies for discovering new antimicrobial agents. Vildagliptin a dipeptidyl peptidase-4 inhibitor (DPI-4) that used effectively in combination with metformin to control blood glucose levels diabetic patients. This study was designed evaluate anti-virulence activities this against clinically important pathogens, Pseudomonas aeruginosa. current findings show significant ability vildagliptin-metformin diminish biofilm formation, bacterial motility, and production virulent extracellular enzymes pyocyanin pigment. Furthermore, drug significantly increased susceptibility P. aeruginosa oxidative stress, indicating immunity enhancement eradication cells. In compliance vitro findings, histopathological photomicrographs mice showed considerable protective effect metformin-vildagliptin aeruginosa, revealing relief inflammation due aeruginosa-induced pathogenesis. mainly employs quorum sensing (QS) systems its huge arsenal virulence factors. can be interrupted by anti-QS both vildagliptin, as exhibited affinity QS receptors. Additionally, downregulated expression main three QS-encoding genes These at very low concentrations (10, 1.25 mg/mL, respectively) compared (850, 50 diabetes.

Язык: Английский

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