Comprehensive Metabolite Profiling and Evaluation of Anti-nociceptive and Anti-inflammatory Potencies of Nypa fruticans (Wurmb.) Leaves: Experimental and In-Silico Approaches.

Heliyon, Год журнала: 2025, Номер 11(3), С. e42074 - e42074

Опубликована: Янв. 21, 2025

Globally, inflammation and pain are among the most prevalent health issues. The use of medicinal plants to alleviate these conditions is growing. This study comprehensively investigated analgesic anti-inflammatory properties ethyl-acetate extract Nypa fruticans (EENF) leaves, traditionally used in folk Medicine. High-performance liquid chromatography (HPLC) gas chromatography-mass spectroscopy (GC-MS) were employed identify phytochemicals EENF. In vitro antioxidant studies conducted determine antioxidative Formalin-induced paw edema assay was assess in-vivo activity whereas; acetic acid-induced writhing test, hot plate tail immersion test performed evaluate vivo anti-nociceptive effects. identified compounds subsequently evaluated by computational against cyclooxygenase-2 enzyme. EENF demonstrated significant both DPPH scavenging (IC50: 105.18 μg/mL) reducing power (RC50:1752.76 μg/mL). assay, exhibited highest (50.39 % 67.72 %) inhibition at fourth hour 200 400 mg/kg body weight, accordingly. Moreover, all modulation (p < 0.001) a dose-dependent manner. Among, 23 phytocompounds, promising ones determined be potential agents through molecular docking ADME/T analysis. Molecular dynamics simulations (MDS) confirmed stability protein-ligand complexes. Two phytochemicals, (-) Epicatechin (CID 72276) Quercetin 5280343), outperformed standard drug, diclofenac sodium, MDS studies. Both experimental in-silico have scientifically verified traditional treating inflammatory disease. Overall, possess excellent as natural lead for COX-2 inhibition. Further research, including pure compound isolation biomolecular studies, needed understand underlying mechanisms activities.

Язык: Английский

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Exploring the anticancer potential of Hewittia malabarica through phytochemical analysis and molecular docking study

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 3, 2025

Язык: Английский

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State of the art progress of Evolvulus alsinoides in pharmacological activity and plant tissue culture: A potent Chinese medicinal plant

Pharmacological Research - Modern Chinese Medicine, Год журнала: 2025, Номер unknown, С. 100586 - 100586

Опубликована: Фев. 1, 2025

Язык: Английский

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Isolation, characterization and pharmacological potentials of methanol extract of Cassia fistula leaves: Evidenced from mice model along with molecular docking analysis

Heliyon, Год журнала: 2024, Номер 10(7), С. e28460 - e28460

Опубликована: Март 27, 2024

The purpose of the current investigation was to conduct a detailed analysis chemical components and medicinal properties methanolic crude extract derived from leaves Cassia fistula. This carried out using both experimental (in vivo) computational silico) methods. Eleven chemicals were chromatographically isolated GC-MS/MS, which utilizes library NIST Wiley 2020 versions. FTIR performed identify functional group compounds. glucose-lowering capacity, analgesic, anti-diarrheal activities analyzed utilizing well-known oral glucose tolerance test, tail immersion method, writhing assay, castor oil-induced diarrheal mice methods, respectively. After 60 min, 120 180 min loading drugs, significant reduction blood levels examined (p < 0.05) in all extracts this plant (200 mg/kg, 400 mg/kg 600 mg/kg) utilized research at time-dependent manner. Similarly, showed effects against pain centrally peripherally compared standard drug morphine (2 bw) diclofenac sodium (50 bw). Moreover, methanol (400 manifested efficacy by inhibiting 72.0 % episode loperamide (inhibition = 80.0%). results investigations corroborated existing in-vivo findings. Greater or close equivalent binding affinity active sites kappa opioid receptor, transporter 3 (GLUT 3), cyclooxygenase 2 indicative potential anti-diarrheal, hypoglycemic, analgesic characteristics compounds (COX-2). anticancer antimicrobial potentiality also found impressive through evaluation with epidermal growth factor receptor (EGFR) dihydrofolate reductase (DHFR) receptors. Results study indicated that C. fistula might be beneficial natural resource for treating diarrhea, hyperglycemia, pain. However, additional is required comprehensive phytochemical screening establish precise action mechanisms plant-derived

Язык: Английский

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Phytochemicals derived from Nicotiana tabacum L. plant contribute to pharmaceutical development

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Апрель 12, 2024

The Nicotiana tabacum L. plant, a medicinal resource, holds significant potential for benefiting human health, as evidenced by its use in Native American and ancient Chinese cultures. Modern medical pharmaceutical studies have investigated that the abundant distinctive function metabolites tobacco including nicotine, solanesol, cembranoid diterpenes, essential oil, seed oil other extracts, avoiding toxic components of smoke, mainly anti-oxidation, anti-lipid production, pro-lipid oxidation, pro-insulin sensitivity, anti-inflammation, anti-apoptosis antimicrobial activities. They showed value supplements or substitutes treating neurodegenerative diseases Alzheimer’s Parkinson’s disease, inflammatory colitis, arthritis, sepsis, multiple sclerosis, myocarditis, metabolic syndrome Obesity fatty liver. This review comprehensively presents research status molecular mechanisms basing on almost all English literature recent 20 years field medicine pharmacology. serves foundation future plants.

Язык: Английский

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An Efficient In Vitro Shoot Organogenesis and Comparative GC-MS Metabolite Profiling of Gaillardia pulchella Foug

Horticulturae, Год журнала: 2024, Номер 10(7), С. 728 - 728

Опубликована: Июль 11, 2024

Gaillardia pulchella Foug. is a widely studied plant because of its high pharmacological and ornamental value. The leaves G. were used for inducing callus subsequent regeneration as it the primary source phytocompounds. purpose present investigation was to formulate an in vitro propagation method by using leaf explants MS (Murashige Skoog) medium. best induction observed on (2.0 mg/L) α-naphthalene acetic acid (NAA) low (0.5 6-benzylaminopurine (BAP) with frequency 91.66%. also demonstrated caulogenesis ability (95.83%), average 5.2 shoots/callus mass at 0.5 mg/L BAP 2.0 NAA. Indole Acetic (IAA) 1.0 had maximum rooting percentage (79.17%) 12.4 roots per shoot. Rooted plantlets later transferred greenhouse conditions, showing survivability rate 75–80%. physiological parameters, i.e., phenolic compounds flavonoids’ level, DPPH assay higher obtained compared formed from field-obtained (mother) leaves. Gas chromatography–mass spectrometry (GC–MS) analysis methanol extracts (in vivo vitro) presented wide array compounds. In extract, some 34 phytocompounds identified. Some them 3-hydroxy-2,3-dihydromaltol (25.39%), isoamyl acetate (11.63%), palmitic (11.55%), 4-methyloxazole (7.54%), 5-methoxypyrrolidin-2-one (7.49%). Leaves derived 45 28 phytocompounds, respectively, belonging different classes like lignans, phenols, terpenoids, alkaloids fatty acids, etc. Those findings that are potential stable several medicinal importance. developed protocol may provide alternative without affecting wild flora.

Язык: Английский

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Evaluation of antibacterial, antioxidant, and anti-inflammatory properties of GC/MS analysis of extracts of Ajuga. integrifolia Buch.-Ham. leaves

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 20, 2024

Abstract The purpose of the current study was to examine chemical composition, antioxidant, anti-inflammatory and antibacterial properties leaves extract Ajuga integrifolia Buch.-Ham. antioxidant three different solvents i.e. methanol (AIM), hexane (AIH), water (AIW) were tested against two bacterial strains Staphylococcus aureus Escherichia coli . presence chemicals, such as hexanedioic acid, hexadecanoic nonadecadiene, hexadecen-1-ol, octadecadienoic nonane, phytol, henicosanal, stearyl aldehyde, neophytadiene, depicted in GCMS chromatograms extracts. After extracts' FTIR peaks examined, it discovered that phenols, amines, hydroxy groups, components linked amino acids present. Compared Hexane Water extracts, Methanol showed a greater phenolic (196.16 ± 0.0083 mg gallic acid equivalent/gram), flavonoid (222.77 0.002 rutin equivalents/g) phosphomolybdate assay for total activity (557.62 0.0023 AAE/g). highest scavenging with minimum IC50 value observed DPPH assay. AIM its maximum anti-denaturation 3.75 0.28%. For activities, best zone inhibition (ZOI) inhibitory concentration (MIC) case compared other extracts methicillin-resistant β-lactam-resistant

Язык: Английский

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Bioactive compounds extracted from leaves of G. cyanocarpa using various solvents in chromatographic separation showed anti-cancer and anti-microbial potentiality in in silico approach

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION), Год журнала: 2023, Номер 51(12), С. 100336 - 100336

Опубликована: Окт. 30, 2023

The study was carried out to determine the possible bioactive compounds from Ethanol, Methanol, Petroleum ether, and Dichloromethane fractions of Glycosmic Cyanocarpa. Analysis these extracts performed using a mass spectrometer detector installed with gas chromatography (GC-MS/MS) utilizing method named electron impact ionization (EI). spectrum each extract compared against information incorporated in library (NIST Wiley) which provides chemical structure name molecular masses identified compounds. A total 73 (25 ethanol, 19 methanol 5 DCM, 24 petroleum ether fractions) were various plants. FTIR analysis crude done identify functional groups plant derived isolated subjected comparison standard drugs towards active binding sites epidermal growth factor receptor (EGFR) dihydrofolate reductase (DHFR) proteins for further evaluation their cytotoxic antimicrobial activity, respectively. Stigmasterol, beta-Sitosterol, Pyrazol-5-amine, 3-methyl-1,4-diphenyl-, 1,2-Benzenediol,4-(2-aminopropyl)-18,19-Secoyohimban-19-oic acid, Phenol, 2,4-bis(1,1-dimethylethyl)-, phosphite (3:1) showed maximum affinity EGFR 16,17,20,21-tetradehydro-16-(hydroxymethyl)-, methyl ester, (15 beta.,16E)-, 3-methyl-1,4-diphenyl- revealed highest DHFR receptor. According current research, G. cyanocarpa may be useful natural source controlling antibacterial anticancer activity. For thorough phytochemical screening determining precise mechanisms action, additional research is required.

Язык: Английский

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Metabolite profiling of potential bioactive fractions from ethanol extract of Boehmeria nivea flowers by GC–MS/MS analysis

Phytomedicine Plus, Год журнала: 2024, Номер 4(2), С. 100557 - 100557

Опубликована: Март 27, 2024

Ramie (Boehmeria nivea) has been reported to have a wide variety of secondary metabolite compounds in its vegetative organ that distinct organic activities. Regarding the chemical composition flowers, there no study considering their pharmacological effects. This research aims determine profile metabolites contained B. nivea flowers employing metabolomics approach. was designed obtain data base from five dissolved fractions ramie flower using Gas Chromatography-Mass Spectrometry (GC-MS/MS) instrument. includes profiles each fraction spectrum obtained, which are then investigated for potential bioactivity based on previously studies. Metabolite were developed Principal Component Analysis (PCA) information clustering and determination marker analyzed. The active separated utilizing maceration method with ethanol solvent multilevel fractionation n-hexane, dichloromethane, ethyl acetic acid derivation, butanol, water GC-MS/MS optimized total represented diversity range 60% - 65%. allowed identification characteristic similarities compound substances accross fractions. interpretation showed dominant n-butanol, acetate, 9,12-Octadecadienoic acid, ethanol, 2-phenoxy-, butyl ester, hexanedioic bis(2-ethylhexyl) ester. PCA combined similarity acetate also butanol n-hexane. Meanwhile, dichloromethane very small content similarities. score plot indicated are: 2-butoxy; hexane, 2,2,3-trimethyl-; phthalic (2-propylpentyl) ester; n-hexadecanoic Hexanedioic bis(2- ethylhexyl) abundance shows application as source new drug candidates. In addition, still many unknown structures or biological activities could lead further provide clues towards bioprospecting plant. CH2Cl2, dichloromethane; Dm1, Dimension 1; Dm2, 2; EtOAc, acetate; GC-MS/MS, Chromatography–Mass Spectrometry; n-BuOH, n-butanol; PCA, Analysis.

Язык: Английский

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Phytochemical profiling, antioxidant and antimicrobial properties of Kumaun Himalayan Artemisia nilagirica (C.B. Clark) Pamp

Natural Product Research, Год журнала: 2025, Номер unknown, С. 1 - 6

Опубликована: Янв. 5, 2025

The aim of the present study was to demonstrate phytochemical characterisation, antioxidant and antimicrobial properties methanol extracts leaf parts A. nilagirica. Phytochemical characterisation by GC-MS analysis revealed presence 30 phytoconstituents such as Methyl commate A (14.69%), Phytol (13.91%), 8,9-Dihydro-9- vinyl-cycloisolongifoline (7.86%), Neophytadine (6.18%), Alpha- Amyrin (5.52%) Squalene (4.99%), 9-Acetyl-S-octahydrophenanthrene (4.62%), Olean-12-en-3-one (3.70%), Acetyloxyparthenin (3.60%) main components. For activity, extract demonstrates IC50 value 8.80 ± 0.03 µg/ml using DPPH, 30.21 0.1 MCA, 7.62 0.01 FRAP scavenging assay respectively. anti-bacterial potential against 2 Gram-negative Gram-positive pathogenic bacterial strains show that has significant inhibition with mean zone in range 16 20 mm MIC values 31.25 125 µg/ml. quantitative analysis, highest Total Phenolic Content (TPC), 372.3 mg GAE/g, Flavonoid (TFC-1), 287.35 QE/g Flavonol (TFC-2), 138.43 dry were found for respectively

Язык: Английский

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