Co-Hydrothermal Carbonization of Sawdust and Sewage Sludge: Assessing the Potential of the Hydrochar as an Adsorbent and the Ecotoxicity of the Process Water

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(3), P. 1052 - 1052

Published: Jan. 21, 2025

Hydrothermal carbonization (HTC) is a promising thermochemical process to convert residues into hydrochar. While conventional HTC utilizes one type of residue as raw material only, Co-HTC generally combines two. By mixing dry and wet wastes, can advantageously avoid water addition. Therefore, this work investigated the potential hydrochar derived from sawdust non-dewatered sewage sludge dye (methylene blue) adsorbent evaluated toxicity resulting (PW) on Daphnia magna. Three hydrochars were produced by at 180, 215, 250 °C named H-180, H-215, H-250, respectively. For methylene blue adsorption, H-180 H-215 had better performance than H-250. Both presented maximum adsorption capacity approximately 70 mg·g−1, which was superior compared with other in literature. Moreover, removal percentage obtained remained satisfactory even after five cycles. Regarding toxicological assays PWs, raising temperature increased variety substances PW composition, higher D. The EC50 values PW-180, PW-215, PW-250 1.13%, 0.97%, 0.51%, This highlights importance searching for treatment valorization PW. Instead viewing by-product an effluent be treated disposed of, it imperative assess PWs obtaining added-value products.

Language: Английский

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Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production

Biotechnology Advances, Journal Year: 2025, Volume and Issue: 79, P. 108523 - 108523

Published: Jan. 31, 2025

Language: Английский

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Deep Eutectic Solvent Pretreatment and Green Separation of Lignocellulose

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(17), P. 7662 - 7662

Published: Aug. 30, 2024

Plant-based waste biomass with lignocellulose as an important component is produced in large quantities worldwide every year. The components of that typically exhibit high utilization value include cellulose and hemicellulose, well pentoses hexoses derived from their hydrolysis. As a pretreatment for the hydrolysis process, delignification pivotal step to enhance cellulose/hemicellulose accessibility achieve yields fermentable sugars. Additionally, deep eutectic solvents (DESs) are most widely used during fractionation due clean environmentally friendly attributes. DESs dissolve lignin by inducing amount β-O-4 bond cleavage partial carbon–carbon cleavage, retaining solid residue, while hemicellulose hydrolyzed DES pretreatment. This article provides comprehensive review influence separation process. Key factors such removal rate, sugar conversion product chemical structure critically reviewed assess feasibility employing separation.

Language: Английский

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Butanol– Product of Agricultural Plant Waste Processing

Waste and Biomass Valorization, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

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High Ectoine Production from Lignocellulosic Hydrolysate by Escherichia coli through Metabolic and Fermentation Engineering

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Ectoine, a major compatible solute in halophilic micro-organisms, shows great potential cosmetics and pharmaceuticals areas owing to its water-binding properties capability prevent oxidative damage. In this study, the ectABC gene cluster responsible for ectoine synthesis originated from bacterium Halomonas venusta was first assembled into Escherichia coli. Subsequently, crr PTS knocked out further drive metabolic flux phosphoenolpyruvate oxaloacetate, resulting 1.27 g/L of ectoine. Then, rate-limiting enzyme LysC pathway identified modified. The recombinant E. coli with overexpression feedback-insensitive mutant EclysC* increased titer 2.51 yield 0.37 g/g shake flasks. After medium optimization including carbon nitrogen source, sodium chloride, magnesium sulfate concentration, improved 4.55 g/L. 115.15 0.23 obtained 5.0 L bioreactor through substrate feeding IPTG supplementation fed-batch fermentation. To achieve cost-effective production ectoine, lignocellulosic hydrolysate wheat straw adopted. 134.08 0.33 sugar productivity 3.7 g/L/h finally produced, representing relatively high level renewable resources compared other studies. This study provides valuable insights efficient method industrial-scale production.

Language: Английский

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Bioethanol as an alternative fuels: A review on production strategies and technique for analysis

Energy Conversion and Management X, Journal Year: 2025, Volume and Issue: unknown, P. 100933 - 100933

Published: Feb. 1, 2025

Language: Английский

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Polyhydroxyalkanoate production by engineered Halomonas grown in lignocellulose hydrolysate

Bioresource Technology, Journal Year: 2025, Volume and Issue: 425, P. 132313 - 132313

Published: Feb. 27, 2025

Language: Английский

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Optimization and comparative study of Bacillus consortia for cellulolytic potential and cellulase enzyme activity

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Lignocellulosic biomass, owing to its recalcitrant nature, requires a consortium of enzymes for breakdown. The present study deals with the isolation cellulolytic bacterial strains from landfill leachate collected Pulau Burung site Penang, Malaysia, and consortia were constructed test their efficiency. dinitro salicylate method was used estimation enzyme activity, compared promising strains. combined potential optimized at varying experimental conditions detect maximum activity. results showed that eight reflected hydrolytic activities, these identified by 16S rDNA sequence as Bacillus subtilis, pumilus, proteolyticus, paramycoides, cereus, altitudinis, niacin, thuringiensis. Consortia A included paramycoides high thermophilic inclination optimal temperature 45°C pH 6 highest cellulase activity 0.90 U/ml. B thuringiensis 0.78 U/ml 38°C 6. significant in breakdown cellulose into useful end products. further proved synergistic relationship more favourable bioconversion processes.

Language: Английский

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Mechanism and improvement of yeast tolerance to biomass-derived inhibitors: A review

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108562 - 108562

Published: March 1, 2025

Language: Английский

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Optimization of Squalene Production by Pseudozyma sp. P4-22

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1646 - 1646

Published: April 7, 2025

Squalene is an important bioactive substance widely used in the food, pharmaceutical, and cosmetic industries. Microbial production of squalene has gained prominence recent years due to its sustainability, safety, environmental friendliness. In this study, a mutant strain, Pseudozyma sp. P4-22, with enhanced squalene-producing ability, was obtained through atmospheric room temperature plasma mutagenesis previously screened yeast SD301. The P4-22 strain demonstrated ability produce using various carbon nitrogen sources. We optimized culture conditions by employing cost-effective corn steep liquor as source, optimal pH sea salt concentration medium were determined be 5.5 5 g/L, respectively. Under cultivation conditions, biomass reached 64.42 g/L 2.06 respectively, L fed-batch fermentation. This study highlights potential promising for commercial-scale squalene.

Language: Английский

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