Nanomaterials, Год журнала: 2024, Номер 14(24), С. 2028 - 2028
Опубликована: Дек. 17, 2024
Advancing experimental methodologies to accurately replicate the physiological and pathological characteristics of biological tissues is pivotal in tissue engineering [...]
Язык: Английский
Journal of Extracellular Biology, Год журнала: 2024, Номер 3(11)
Опубликована: Окт. 30, 2024
The translation of pre-clinical anti-cancer therapies to regulatory approval has been promising, but slower than hoped. While innovative and effective treatments continue achieve or seek approval, setbacks are often attributed a lack efficacy, failure clinical endpoints, dose-limiting toxicities. Successful efforts have characterized by the development therapeutics designed specifically deliver optimal dosing tumour cells while minimizing off-target toxicity. Much effort devoted rational design application synthetic nanoparticles serve as targeted therapeutic delivery vehicles. Several challenges successful this modality vehicles include induction protracted immune response that results in their rapid systemic clearance, manufacturing cost, stability, biocompatibility. Extracellular vesicles (EVs) heterogeneous class endogenous biologically produced lipid bilayer mediate intercellular communication carrying bioactive macromolecules capable modifying cellular phenotypes local distant cells. By genetic, chemical, metabolic methods, extracellular can be engineered display targeting moieties on surface transporting specific cargo modulate pathological processes following uptake target cell populations. This review will survey types EVs, composition cargoes, strategies employed increase targeting, uptake, release, potential
Язык: Английский
Процитировано
2
Materials Today Communications, Год журнала: 2024, Номер 39, С. 109318 - 109318
Опубликована: Май 22, 2024
Язык: Английский
Процитировано
2
Nano Letters, Год журнала: 2024, Номер unknown
Опубликована: Сен. 12, 2024
Язык: Английский
Процитировано
2
Immuno, Год журнала: 2024, Номер 4(4), С. 312 - 324
Опубликована: Сен. 27, 2024
Recent advances in organoid technology have revolutionized cancer biology and therapeutic interventions, offering personalized immunotherapy treatment. Organoids, three-dimensional cell cultures derived from patient tumors, accurately replicate the tumor microenvironment, providing unprecedented insights into tumor-immune interactions responses. In this literature-based study, we discuss various culture methods for diverse applications of organoids immunotherapy, including drug screening, treatment strategies, mechanistic studies. Additionally, address technological challenges associated with these propose potential future solutions to accelerate development novel immunotherapeutic approaches. This review highlights transformative models advancing preclinical modeling, evaluation, paving way more effective treatments.
Язык: Английский
Процитировано
1
Life, Год журнала: 2024, Номер 14(12), С. 1552 - 1552
Опубликована: Ноя. 26, 2024
Despite the promising potential of cell-based therapies developed using tissue engineering techniques to treat a wide range diseases, including limbal stem cell deficiency (LSCD), which leads corneal blindness, their commercialization remains constrained. This is primarily attributable limited sources, use non-standardizable, unscalable, and unsustainable techniques, extended manufacturing processes required produce transplantable tissue-like surrogates. Herein, we present first demonstration novel approach combining collagen films (CF), hyaluronic acid (HA), human telomerase-immortalized epithelial cells (T-LESCs), macromolecular crowding (MMC) develop innovative biomimetic substrates for (LESCs). The initial step involved fabrication characterization CF enriched with HA (CF-HA). Subsequently, T-LESCs were seeded on CF, CF-HA, culture plastic (TCP). Thereafter, effect these matrices basic cellular function tissue-specific extracellular matrix (ECM) deposition or without MMC was evaluated. viability metabolic activity cultured TCP found be similar, while CF-HA induced highest (p < 0.05) proliferation. It notable that growth, whereas increased IV, fibronectin, laminin in T-LESC culture. data highlight of, particular, immortalized development substrates, could utilized ocular surface reconstruction following further vitro, vivo, clinical validation approach.
Язык: Английский
Процитировано
1
Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12
Опубликована: Ноя. 26, 2024
Despite extensive efforts to unravel tumor behavior and develop anticancer therapies, most treatments fail when advanced clinical trials. The main challenge in cancer research has been the absence of predictive models, accurately mimicking tumoral processes response treatments. microenvironment (TME) shows several human-specific physical chemical properties, which cannot be fully recapitulated by conventional 2D cell cultures or
Язык: Английский
Процитировано
1
Carbohydrate Polymer Technologies and Applications, Год журнала: 2024, Номер unknown, С. 100640 - 100640
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
1
Опубликована: Авг. 12, 2024
Recent advances in organoid technology have revolutionized cancer biology and thera-peutic interventions, offering personalized immunotherapy treatment. Organoids, three-dimensional cell cultures derived from patient tumors, accurately replicate the tumor microenvi-ronment, providing unprecedented insights into tumor-immune interactions therapeutic re-sponses. In this literature-based study, we discuss various culture methods for diverse appli-cations of organoids immunotherapy, including drug screening, treatment strategies, mechanistic studies. Additionally, address technological challenges asso-ciated with these propose potential future solutions to accelerate development novel immunotherapeutic approaches. This review highlights transformative or-ganoid models advancing preclinical modeling, evalua-tion, paving way more effective treatments.
Язык: Английский
Процитировано
0
Bioengineering, Год журнала: 2024, Номер 11(9), С. 902 - 902
Опубликована: Сен. 9, 2024
Three-dimensional cancer cell cultures have been a valuable research model for developing new drug targets in the preclinical stage. However, there are still limitations to these vitro models. Scaffold-based systems offer promising approach overcoming challenges research. In this study, we show that two-photon polymerization (TPP)-assisted printing of scaffolds enhances 3D tumor culture formation without additional modifications. TPP is perfect fit task, as it an advanced 3D-printing technique combining μm-level resolution with complete freedom design final structure. Additionally, can use wide array materials, including biocompatible ones. We exploit capabilities fabricate from two different materials—PEGDA and OrmoClear. Cubic spheroid more complex architecture were produced tested. The biological evaluation showed human ovarian lines SKOV3 A2780 formed on printed preference material. gene expression line exhibited substantial changes CDH1, CDH2, TWIST, COL1A1, SMAD3 expression, while had slight said expression. Our findings how scaffold impacts formation, especially line.
Язык: Английский
Процитировано
0
Nanomaterials, Год журнала: 2024, Номер 14(24), С. 2028 - 2028
Опубликована: Дек. 17, 2024
Advancing experimental methodologies to accurately replicate the physiological and pathological characteristics of biological tissues is pivotal in tissue engineering [...]
Язык: Английский
Процитировано
0