Two-Stage Recognition Mechanism of the SARS-CoV-2 Receptor-Binding Domain to Angiotensin-Converting Enzyme-2 (ACE2)

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(1), С. 679 - 679

Опубликована: Янв. 4, 2024

The SARS-CoV-2 virus, commonly known as COVID-19, occurred in 2019. It is a highly contagious illness with effects ranging from mild symptoms to severe illness. also one of the best-known pathogens since more than 200,000 scientific papers last few years. With publication (SARS-CoV-2-CTD) spike (S) protein complex human ACE2 (hACE2) (PDB (6LZG)), molecular analysis most crucial steps on infection pathway was possible. aim this manuscript simulate widely spread mutants SARS-CoV-2, namely Alpha, Beta, Gamma, Delta, Omicron, and first recognized variant (natural wild type). wide search hypersurface potential energy performed using UNRES force field, intermediate state ACE2–RBD found. R403, K/N/T417, L455, F486, Y489, F495, Y501, Y505 played role recognition mechanism. cannot be very stable it will prevent cascade.

Язык: Английский

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CCN1-Mediated Signaling in Placental Villous Tissues after SARS-CoV-2 Infection in Term Pregnant Women: Implications for Dysregulated Angiogenesis

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(4), С. 3533 - 3550

Опубликована: Апрель 18, 2024

The global spread of SARS-CoV-2 has increased infections among pregnant women. This study aimed to explore placental pathology alterations and angiogenic factor levels in term women after infection a retrospective single-center study. Additionally, we investigated the role underlying mechanism vascular inflammation-promoting, cysteine-rich protein 61 (CYR61/CCN1) this context. All analyses were performed infected with or without SARS-CoV-2. sFlt-1, PlGF, sEng serum quantified using ELISA. Placental expressions examined by immunoblot immunostaining. effect CCN1 on SGHPL-5 trophoblast cells was examined. We found that activated inflammatory response women, leading pronounced villous tissues. Elevated anti-angiogenic factors (sFlt-1, sEng) upon may directly contribute these pathological changes. Upregulated pNF-κB tissues patients are identified as crucial alterations. As conclusion, significantly elevated placentas By activating cascade responses, induced production sFlt-1 sEng, which lead abnormal architecture.

Язык: Английский

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Dedicated Protocol for Ultrastructural Analysis of Farmed Rainbow Trout (Oncorhynchus mykiss) Tissues with Red Mark Syndrome: The Skin—Part One

Methods and Protocols, Год журнала: 2024, Номер 7(3), С. 37 - 37

Опубликована: Апрель 24, 2024

The present study aims to provide a specific protocol for transmission electron microscopy of sample skin rainbow trout affected by red mark syndrome (RMS). is disease that affects the (Oncorhynchus mykiss). disease, probably due Midichloria-like organism infection, not lethal, but morbidity can reach up 60%, leading significant economic impact associated with downgrading commercial product, increased labor, and susceptibility secondary infections. ultrastructure analyses allowed an earlier identify presence scattered microorganisms characterized oval shape, mainly in cytoplasm cells. developed this will be instrumental visualizing microorganism, which responsible infection.

Язык: Английский

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Detection of SARS-CoV-2 Viral Genome and Viral Nucleocapsid in Various Organs and Systems

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 5755 - 5755

Опубликована: Май 25, 2024

While considerable attention has been devoted to respiratory manifestations, such as pneumonia and acute distress syndrome (ARDS), emerging evidence underlines the significance of extrapulmonary involvement. In this study, we examined 15 hospitalized patients who succumbed severe complications following SARS-CoV-2 infection. These were admitted Sibiu County Clinical Emergency Hospital in Sibiu, Romania, between March October 2021. All ethnic Romanians. Conducted within a COVID-19-restricted environment adhering national safety protocols, autopsies provided comprehensive understanding disease’s multisystemic impact. Detailed macroscopic evaluations histopathological analyses myocardial, renal, hepatic, splenic, gastrointestinal tissues performed. Additionally, reverse transcription-quantitative polymerase chain reaction (rt-qPCR) assays immunohistochemical staining employed detect viral genome nucleocapsid tissues. Myocardial lesions, including ischemic microstructural changes inflammatory infiltrates, prevalent, indicative COVID-19’s cardiac implications, while renal pathology revealed chronic alterations, tubular necrosis, infiltrates most evident. Hepatic examination identified hepatocellular necroinflammatory hepatocytic cytopathy, highlighting hepatic involvement Splenic parenchymal disorganization was prominent, indicating systemic immune dysregulation. Furthermore, examinations unveiled nonspecific changes. Molecular detected genes various organs, with confirming presence predominantly macrophages fibroblasts. findings highlighted nature infection, emphasizing need for clinical management strategies targeted therapeutic approaches beyond systems.

Язык: Английский

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A Comparison between SARS-CoV-2 and Gram-Negative Bacteria-Induced Hyperinflammation and Sepsis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(20), С. 15169 - 15169

Опубликована: Окт. 14, 2023

Sepsis is a life-threatening condition caused by the body’s overwhelming response to an infection, such as pneumonia or urinary tract infection. It occurs when immune system releases cytokines into bloodstream, triggering widespread inflammation. If not treated, it can lead organ failure and death. Unfortunately, sepsis has high mortality rate, with studies reporting rates ranging from 20% over 50%, depending on severity promptness of treatment. According World Health Organization (WHO), annual death toll in world about 11 million. One main toxins responsible for inflammation induction are lipopolysaccharides (LPS, endotoxin) Gram-negative bacteria, which rank among most potent immunostimulants found nature. Antibiotics consistently prescribed part anti-sepsis-therapy. However, antibiotic therapy (i) increasingly ineffective due resistance development (ii) antibiotics unable bind neutralize LPS, prerequisite inhibit interaction endotoxin its cellular receptor complex, namely Toll-like 4 (TLR4)/MD-2, intracellular cascade leading pro-inflammatory cytokine secretion. The pandemic virus SARS-CoV-2 infected hundreds millions humans worldwide since emergence 2019. COVID-19 (Coronavirus disease-19) this associated lethality, particularly elderly immunocompromised people. As August 2023, nearly 7 million deaths were reported disease. some studies, upregulation TLR4 subsequent inflammatory signaling detected patients “mimics bacterial sepsis”. Furthermore, was described others “mirror image Similarly, profile sera severe very similar those suffering acute respiratory distress syndrome (ARDS) sepsis. Finally, infection frequently accompanied co-infections, well presence significant LPS concentrations. In present review, we will analyze similarities differences between at pathophysiological, epidemiological, molecular levels.

Язык: Английский

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Metabolic Deregulation in Pulmonary Hypertension

Current Issues in Molecular Biology, Год журнала: 2023, Номер 45(6), С. 4850 - 4874

Опубликована: Июнь 3, 2023

The high morbidity and mortality rate of pulmonary arterial hypertension (PAH) is partially explained by metabolic deregulation. present study complements our previous publication in "Genes" identifying significant increases the glucose transporter solute carrier family 2 (Slc2a1), beta nerve growth factor (Ngf), nuclear erythroid-derived 2-like (Nfe2l2) three standard PAH rat models. was induced subjecting animals to hypoxia (HO), or injecting with monocrotaline either normal (CM) hypoxic (HM) atmospheric conditions. Western blot double immunofluorescent experiments were complemented novel analyses previously published transcriptomic datasets animal lungs from perspective Genomic Fabric Paradigm. We found substantial remodeling citrate cycle, pyruvate metabolism, glycolysis/gluconeogenesis, fructose mannose pathways. According distance, glycolysis/gluconeogenesis most affected functional pathway all decoupled coordinated expression many genes, replaced phosphomannomutase (Pmm2) 1 (Pmm1) center metabolism. also regulation key genes involved channelopathies. In conclusion, data show that dysregulation a major pathogenic factor.

Язык: Английский

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The COVID-19 pandemic and its possible impact on the treatment of odontogenic and intraoral abscesses

Head & Face Medicine, Год журнала: 2023, Номер 19(1)

Опубликована: Авг. 19, 2023

Most odontogenic and intraoral abscesses can be treated on an outpatient basis with local anesthesia. However, severe disease progression may require incision under general anesthesia (GA) postoperative inpatient treatment. This study aimed to evaluate the first "COVID-19 year" in Germany compare two previous years. All consecutive cases or setting between 2018 2021 were included this study. Data collected, including type of anesthesia, length hospital stay, healthcare costs. Despite lower total number abscess treatments year COVID-19 (n = 298 patients) than that years 663 patients), advanced requiring intervention GA was significantly higher (p < 0.001). increased burden care also reflected The measures taken against pandemic had impact course other diseases, for example, abscesses. results showed emerging conflict patient during crisis should considered possible future pandemics.

Язык: Английский

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Acute orchitis deciphered: Coxsackievirus B strains are the main etiology and their presence in semen is associated with acute inflammation and risk of persistent oligozoospermia

Journal of Medical Virology, Год журнала: 2023, Номер 95(7)

Опубликована: Июль 1, 2023

Abstract Although various viruses are considered to be the clinical cause for acute orchitis, it is completely unclear what extent and which etiologically involved in orchitis clinic course of these patients like. Therefore, a prospective study was set up decipher isolated orchitis. Between July 2007 February 2023, total 26 with were recruited compared 530 epididymitis. We able show that (1) usually viral origin (20/26, 77%) enteroviruses coxsackievirus B strains (16/26, 62%) predominant, (2) virus isolates could received from semen indicating presence replication‐competent particles, (3) polymerase chain reaction (PCR) should conducted using provided at onset disease, because not detectable serum/urine, (4) there circannual occurrence maximum summer, (5) associated characteristic inflammatory cytokine panel systemic inflammation, (6) rapidly self‐limiting, (7) about 30% (6/20) suffer ongoing oligozoospermia. These seven emerging aspects likely fundamentally change thinking practice regarding

Язык: Английский

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Reliability of single-leg maximal dynamic strength performance and inter-limb asymmetries in pre-pubertal soccer players. The influence of maturity in asymmetries

Heliyon, Год журнала: 2024, Номер 10(19), С. e38716 - e38716

Опубликована: Сен. 28, 2024

Язык: Английский

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Update on andrological effects of SARS‐CoV‐2 infection and COVID‐19: An overview review

Andrology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 31, 2024

Evidence indicates a wide range of andrological alterations in patients with the Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infection and Coronavirus Disease 2019 (COVID-19).

Язык: Английский

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