Exploratory Review and In Silico Insights into circRNA and RNA-Binding Protein Roles in γ-Globin to β-Globin Switching
Cells,
Год журнала:
2025,
Номер
14(4), С. 312 - 312
Опубликована: Фев. 19, 2025
β-globin
gene
cluster
regulation
involves
complex
mechanisms
to
ensure
proper
expression
and
function
in
RBCs.
During
development,
switching
occurs
as
γ-globin
is
replaced
by
β-globin.
Key
regulators,
like
BCL11A
ZBTB7A,
repress
facilitate
this
transition
with
other
factors,
KLF1,
LSD1,
PGC-1α;
these
regulators
an
orchestrated
from
γ-
during
development.
While
have
been
extensively
studied,
circRNAs
recently
emerged
key
contributors
regulation,
but
their
role
remains
largely
unexplored.
Although
discovered
the
1970s,
only
recognized
for
functional
roles,
particularly
interactions
RNA-binding
proteins.
Understanding
how
contribute
could
lead
new
therapeutic
strategies
hemoglobinopathies,
such
sickle
cell
disease
β-thalassemia.
This
review
uses
circAtlas
3.0
database
explore
circRNA
expressions
genes
related
expression,
focusing
on
blood,
bone
marrow,
liver,
spleen.
It
emphasizes
exploration
of
potential
between
proteins
involved
regulatory
mechanisms,
further
enhancing
our
understanding
expression.
Язык: Английский
Circular RNAs modulate cancer drug resistance: advances and challenges
Cancer Drug Resistance,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 28, 2025
Acquired
drug
resistance
is
a
main
factor
contributing
to
cancer
therapy
failure
and
high
mortality,
highlighting
the
necessity
develop
novel
intervention
targets.
Circular
RNAs
(circRNAs),
an
abundant
class
of
RNA
molecules
with
closed
loop
structure,
possess
characteristics
including
stability,
which
provide
unique
advantages
in
clinical
application.
Growing
evidence
indicates
that
aberrantly
expressed
circRNAs
are
associated
against
various
treatments,
targeted
therapy,
chemotherapy,
radiotherapy,
immunotherapy.
Therefore,
targeting
these
aberrant
may
offer
strategy
improve
efficiency
therapy.
Herein,
we
present
summary
most
recently
studied
their
regulatory
roles
on
resistance.
With
advances
artificial
intelligence
(AI)-based
bioinformatics
algorithms,
could
emerge
as
promising
biomarkers
targets
Язык: Английский
Advancing mRNA vaccines for infectious diseases: key components, innovations, and clinical progress
Essays in Biochemistry,
Год журнала:
2025,
Номер
69(02)
Опубликована: Май 1, 2025
Vaccination
remains
a
cornerstone
in
preventing
infectious
diseases
and
managing
outbreaks.
The
COVID-19
pandemic
has
underscored
the
revolutionary
impact
of
mRNA
vaccine
technology,
which
utilizes
pathogenderived
genomic
sequences
to
generate
specific
antigens.
This
process
involves
vitro
transcription
mRNA,
encoding
target
antigens
that
are
subsequently
encapsulated
within
lipid
nanoparticles
(LNPs)
for
efficient
delivery
into
host
cells.
Once
internalized,
enables
antigen
expression,
triggering
robust
immune
response.
platform
dramatically
accelerates
development
timelines
offers
unparalleled
adaptability,
making
vaccines
particularly
advantageous
addressing
emerging
diseases.
clinical
success
BNT162b2
(Pfizer-BioNTech)
mRNA-1273
(Moderna)
fueled
broader
applications,
including
influenza,
respiratory
syncytial
virus
(RSV),
Zika,
HIV.
Notably,
mRNA-1345
became
first
FDA-approved
RSV
vaccine,
while
self-amplifying
RNA
multivalent
advancing
trials.
However,
CureVac’s
CVnCoV
failed
due
lack
nucleoside
modifications,
mRNA-1325
(Zika)
showed
poor
immunogenicity.
Additionally,
mRNA-1365
(RSV)
faced
an
FDA
hold
safety
concerns.
These
cases
highlight
need
continued
optimization
sequence
design,
delivery,
assessment.
Despite
advancements,
key
hurdle
persists,
instability,
ultra-low
storage
requirements,
LNP
liver
accumulation.
Innovations
such
as
lyophilization
selective
organ
targeting
technology
being
explored
improve
stability
extrahepatic
delivery.
review
examines
strategies,
progress,
challenges,
providing
insights
future
developments
this
evolving
field.
Язык: Английский
A Complete Approach for circRNA Therapeutics from Purification to Lyophilized Delivery Using Novel Ionizable Lipids
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(11), С. 5138 - 5138
Опубликована: Май 27, 2025
Circular
RNA
(circRNA)
has
gained
significant
attention
as
a
potential
therapeutic
tool
due
to
its
remarkable
stability
and
resistance
degradation
by
exonucleases.
However,
scalable
efficient
methods
for
purification
delivery
remain
critical
challenges
that
must
be
addressed.
In
this
study,
we
developed
evaluated
an
optimized
affinity
chromatography
method
using
Oligo
(dT)
columns
the
of
circRNA,
achieving
high
yield
purity
compared
with
high-performance
liquid
chromatography.
Additionally,
investigated
in
vivo
efficacy
circRNA-Oligo
encapsulated
lipid
nanoparticles
(LNPs)
formulated
emerging
ionizable
lipids,
including
CP-LC-0867
CP-LC-0729.
Our
results
showed
LNPs
consistently
produced
higher
protein
expression
SM-102,
sustained
luciferase
activity
observed
over
14-day
period.
Furthermore,
assessed
lyophilization
LNP-circRNA-Oligo
CP-LC-0729
extend
shelf
life
eliminate
need
ultra-low-temperature
storage.
Remarkably,
lyophilized
exhibited
no
differences
their
non-lyophilized
counterparts,
demonstrating
is
viable
strategy
extending
storage
transport
circRNA
therapies.
These
findings
underscore
new
improved
strategies,
techniques
enhance
scalability,
stability,
practical
application
Язык: Английский
Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication
Plants,
Год журнала:
2024,
Номер
14(1), С. 61 - 61
Опубликована: Дек. 27, 2024
Among
the
long
non-coding
RNAs
that
are
currently
recognized
as
important
regulatory
molecules
influencing
a
plethora
of
processes
in
eukaryotic
cells,
circular
(circRNAs)
represent
distinct
class
predominantly
produced
by
back-splicing
pre-mRNA.
The
most
studied
mechanisms
involving
circRNAs
acting
miRNA
sponges,
forming
R-loops
with
genomic
DNA,
and
encoding
functional
proteins.
In
addition
to
generated
back-splicing,
two
types
capable
autonomous
RNA-RNA
replication
systemic
transport
have
been
described
plants:
viroids,
which
infectious
cause
number
plant
diseases,
retrozymes,
transcripts
retrotransposon
loci
circularization
due
ribozymes.
Based
on
common
features,
viroids
retrozymes
considered
be
evolutionarily
related.
Here,
we
provide
an
overview
biogenesis
functions
non-replicating
further
discuss
detail
available
data
focusing
their
structural
mechanisms,
interaction
cellular
components,
plants.
addition,
biotechnological
approaches
replication-capable
discussed,
well
potential
applications
research
agriculture.
Язык: Английский