Dual ROS/Glucose-Responsive Quercetin-Loaded Supramolecular Hydrogel for Diabetic Wound Healing
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
Diabetic
wound
healing
remains
a
significant
challenge
due
to
complex
pathological
mechanisms,
including
prolonged
inflammation,
excessive
reactive
oxygen
species
(ROS)
accumulation,
angiogenesis
dysfunction,
and
increased
susceptibility
bacterial
infection.
In
this
study,
we
developed
dual
ROS/glucose-responsive
quercetin-loaded
supramolecular
hydrogel
(GPQ
hydrogel)
for
treating
diabetic
wounds.
The
was
fabricated
by
incorporating
quercetin
(QUE)
into
guanosine–phenylboronic
acid
(GP)
network
through
dynamic
borate
ester
bonds.
Structural
characterization
revealed
the
formation
of
typical
G-quadruplex
structure
in
GPQ
hydrogel.
responsiveness
ROS
glucose
enabled
controlled
release
QUE,
effectively
addressing
abnormal
microenvironment
diabetes.
vitro
studies
demonstrated
excellent
antibacterial,
antioxidant,
anti-inflammatory,
pro-angiogenic
properties
Furthermore,
vivo
study
using
full-thickness
model
streptozotocin-induced
rats
showed
that
significantly
accelerated
closure,
enhanced
re-epithelialization
collagen
deposition,
promoted
compared
control
GP
groups.
Immunofluorescence
analysis
confirmed
superior
antioxidant
effects
microenvironment.
This
presents
promising
multifunctional
biomaterial
managing
Язык: Английский
LC-MS analysis and antioxidant, antibacterial, and antidiabetic activity of Jumli Marshi rice from Nepal: An in vitro and in silico investigation to validate their potential as a functional food
PLoS ONE,
Год журнала:
2025,
Номер
20(3), С. e0319338 - e0319338
Опубликована: Март 10, 2025
Jumli
Marshi
(J.
Marshi)
,
a
native
rice
cultivar
in
Nepal,
is
gaining
popularity
owing
to
its
health
benefits
for
obesity,
hypertension,
and
diabetes.
However,
scientific
evidence
verifying
therapeutic
potential
lacking
until
November
2024.
Therefore,
we
aimed
characterize
the
phytoconstituents
evaluate
antioxidant,
antibacterial,
antidiabetic
properties
of
J.
Marshi,
along
with
ADME
toxicity
profile,
using
both
vitro
silico
approaches.
Liquid
chromatography-mass
spectrometry
analysis
70%
methanol
extract
from
identified
ten
plant-based
compounds,
including
phenolic
acids,
flavonoids,
γ-oryzanol.
The
exhibited
significant
antioxidant
properties,
neutralizing
DPPH
free
radicals
fifty-percentage
inhibitory
concentration
(IC
50
)
42.65
±
3.9
µg/mL,
compared
ascorbic
acid’s
IC
4.12
0.7
µg/mL.
It
also
showed
antibacterial
activity
against
Staphylococcus
aureus
zone
inhibition
(ZOI)
ranging
7
11
mm
minimum
(MIC)
1.56
mg/mL,
standard
antibiotics
meropenem
(ZOI:
24
1.6
mm;
MIC:
mg/mL).
enzymatic
assay
demonstrated
that
inhibits
fifty
percent
enzyme
at
(EC
>
1000
µg/mL
α
-amylase
250
2.5
-glucosidase,
contrast
acarbose,
exhibiting
an
EC
35.5
1.5
189.5
1.9
-glucosidase.
In
docking
studies
revealed
strong
interactions
target
protein
catalytic
residues,
particularly
gamma-oryzanol
(−10.0
kcal/mol)
chlorogenic
acid
-glucosidase
(−7.7
kcal/mol),
acarbose
(−6.9
−7.1
kcal/mol).
suggested
tricin
had
best
drug-likeness
safety
profiles.
To
our
knowledge,
this
first
study
reveal
presence
bioactive
acids
flavonoids.
Furthermore,
it
offers
supporting
-glucosidase-inhibitory
confirming
applications
as
functional
food
used
management
Язык: Английский
Effect of Resveratrol and Quercetin on SFRP4 as a Biomarker of Diabesity: In Silico and In Vivo Studies
Biochemical and Biophysical Research Communications,
Год журнала:
2025,
Номер
unknown, С. 151748 - 151748
Опубликована: Апрель 1, 2025
Язык: Английский
Investigation Into the Impact of Solvents on the Phytochemical Composition, Antioxidant Capacities, and Antihyperglycemic Activities of Erigeron annuus (L.) Pers.
BioMed Research International,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
This
study
aims
to
assess
the
phytochemical
composition,
antioxidant
potential,
and
antidiabetic
properties
of
Erigeron
annuus
(L.)
Pers.
The
ethyl
acetate
fraction
leaves
exhibited
highest
extraction
rate
(22.42%).
preliminary
qualitative
analysis
in
crude
extract
fractions
is
often
performed
using
chemical
tests.
For
quantitative
analysis,
spectrophotometric
methods
are
widely
used
estimate
concentration
phytochemicals.
were
evaluated
2,2‐diphenyl‐1‐picrylhydrazyl
(DPPH)
radical
scavenging
assay
ferric
reducing
power
(FRAP)
assay,
which
measures
reduction
Fe
3+
2+
.
Qualitative
screening
revealed
presence
tannins,
flavonoids,
phenols,
saponins,
alkaloids.
Notably,
showed
significantly
(
p
<
0.05)
higher
total
phenolic
content
(70.01
±
1.1
mg/g)
flavonoid
(80.29
1.03
mg/g).
also
demonstrated
substantial
α
‐amylase
inhibitory
activity
suggesting
ability
polyphenols
reduce
activity.
inhibition
(23.15
1.22
%
67.31
2.01
)
IC
50
value
(40.59
0.03
μ
g/mL)
notably
compared
with
standard
drug
metformin
(19.88
1.51
g/mL).
glucose
levels
(10.88
1.29
65.11
0.94
conducted
a
lipid
peroxidation
experiment
utilizing
egg
yolk
as
source
lipids
high
content.
most
bioactive
was
for
cytotoxicity
against
HEK293
cell
line.
that
50%
viability
observed
at
g/mL,
indicating
plant
nontoxic
concentrations
below
this
threshold.
Furthermore,
dominant
further
investigated
liquid
chromatography–mass
spectroscopy
high‐performance
thin‐layer
chromatography
techniques
from
selected
plant.
Moreover,
an
vivo
will
be
evaluate
efficacy
,
isolate
characterize
its
components,
examine
molecular
mechanism
action
improve
therapeutic
applicability.
Язык: Английский
Impacts of Plu kaow (Houttuynia cordata Thunb.) Ethanolic Extract on Diabetes and Dyslipidemia in STZ Induced Diabetic Rats: Phytochemical Profiling, Cheminformatics Analyses, and Molecular Docking Studies
Antioxidants,
Год журнала:
2024,
Номер
13(9), С. 1064 - 1064
Опубликована: Авг. 30, 2024
The
increasing
prevalence
of
diabetes
and
dyslipidemia
poses
significant
health
challenges,
impacting
millions
people
globally
leading
to
high
rates
illness
death.
This
study
aimed
explore
the
potential
antidiabetic
hypolipidemic
effects
Plu
kaow
(
Язык: Английский
Kalanchoe tomentosa: Phytochemical Profiling, and Evaluation of Its Biological Activities In Vitro, In Vivo, and In Silico
Pharmaceuticals,
Год журнала:
2024,
Номер
17(8), С. 1051 - 1051
Опубликована: Авг. 9, 2024
In
this
work,
the
leaves
of
K.
tomentosa
were
macerated
with
hexane,
chloroform,
and
methanol,
respectively.
The
phytochemical
profiles
hexane
chloroform
extracts
unveiled
using
GC/MS,
whereas
chemical
composition
methanol
extract
was
analyzed
UPLC/MS/MS.
antibacterial
activity
determined
against
gram-positive
gram-negative
strains
through
minimal
inhibitory
concentration
assay,
in
silico
studies
implemented
to
analyze
interaction
phytoconstituents
bacterial
peptides.
antioxidant
property
assessed
by
evaluating
their
capacity
scavenge
DPPH,
ABTS,
H2O2
radicals.
toxicity
recorded
Artemia
salina
nauplii
Caenorhabditis
elegans
nematodes.
Results
demonstrate
that
contain
phytosterols,
triterpenes,
fatty
acids,
possesses
glycosidic
derivatives
quercetin
kaempferol
together
sesquiterpene
lactones.
performance
cultured
appraised
as
weak
due
MIC90
values
(>500
μg/mL).
As
antioxidants,
treatment
executed
high
moderate
activities
within
range
50–300
μg/mL.
Extracts
did
not
decrease
viability
A.
salina,
but
they
exerted
a
toxic
effect
C.
during
exposure
treatment.
Through
modeling,
it
flavonoids
contained
can
hamper
NAM/NAG
peptide,
which
is
great
interest
since
determines
formation
peptide
wall
bacteria.
This
study
reports
for
first
time
biological
content
from
proposes
possible
mechanism
Язык: Английский
Antioxidant, Alpha‐Amylase Inhibitory and Hypoglycemic Activity of Smallanthus sonchifolius Leaves from Nepal: An Integrated In Vitro, In Vivo, and In Silico Approach
Food Science & Nutrition,
Год журнала:
2024,
Номер
13(1)
Опубликована: Дек. 26, 2024
In
2019,
diabetes
mellitus
affected
9.3%
of
the
global
population
and
accounted
for
one
in
nine
adult
deaths.
Plant-based
antioxidants
neutralize
harmful
free
radicals,
mitigate
oxidative
stress,
significantly
prevent
its
complications.
This
study
evaluated
vitro
antioxidant,
alpha-amylase
inhibitory,
vivo
oral
hypoglycemic,
Язык: Английский
Lipophilic derivatives of EGCG as potent α-amylase and α-glucosidase inhibitors ameliorating oxidative stress and inflammation
Bioorganic Chemistry,
Год журнала:
2024,
Номер
153, С. 107786 - 107786
Опубликована: Сен. 3, 2024
Язык: Английский
Psidium guajava leaves extract alters colonic microbiome composition and reduces intestinal sodium absorption in rats exposed to a high-sodium diet
Journal of Pharmacy and Pharmacology,
Год журнала:
2024,
Номер
77(1), С. 111 - 126
Опубликована: Ноя. 7, 2024
High
sodium
intake
is
a
major
risk
factor
for
hypertension
and
renal
diseases.
Previous
studies
have
shown
that
suspension
of
ethanolic
extract
Psidium
guajava
(guava)
leaves
(PsE)
has
antihypertensive
effects
in
rats
on
high-sodium
diet
(HSD),
but
some
mechanisms
to
remain
unexplored.
This
study
explored
whether
oral
PsE
treatment
affects
handling
by
the
intestine
alters
gut
microbiome
HSD-fed
rats.
Язык: Английский
Protective Effects of Food-Derived Kaempferol on Pancreatic β-Cells in Type 1 Diabetes Mellitus
Foods,
Год журнала:
2024,
Номер
13(23), С. 3797 - 3797
Опубликована: Ноя. 26, 2024
Background:
Kaempferol
(KPF),
a
flavonoid
abundant
in
edible
plants,
possesses
potent
anti-inflammatory
and
antioxidant
properties
beneficial
with
notable
health
benefits.
Objective:
To
evaluate
the
protective
effects
of
KPF
on
metabolic
disturbances
pancreatic
damage
Type
1
diabetes
mellitus
(T1DM)
mouse
model.
Methods:
Male
C57BL/6
mice
were
divided
into
normal,
T1DM,
T1DM
+
25
mg/kg,
50
mg/kg
groups.
was
induced
by
streptozotocin
(STZ).
administered
via
intraperitoneal
injection
for
2
weeks.
After
4
weeks
from
start,
parameters,
histology,
plasma
metabolites
analyzed.
Network
pharmacology
molecular
docking
identified
key
targets
pathways.
In
vitro,
INS-1
cells
used
to
assess
reactive
oxygen
species
(ROS)
production
apoptosis.
Results:
significantly
reduced
blood
glucose
(GLU)
triglyceride
(TG)
levels,
increased
high-density
lipoprotein
(HDL)
preserved
β-cell
structure.
Metabolomics
revealed
changes
energy
metabolism
oxidative
stress-related
metabolites.
analysis
highlighted
PI3K/AKT/mTOR
pathway,
strong
binding
affinities
such
as
AKT1.
decreased
ROS
cells;
this
effect
reversed
PI3K/AKT
inhibitor.
also
apoptosis
cells.
Conclusions:
ameliorates
mice,
suggesting
potential
functional
food
ingredient
management.
Язык: Английский