Plant-Derived Exosome-like Nanoparticles for Biomedical Applications and Regenerative Therapy

Biomedicines, Год журнала: 2023, Номер 11(4), С. 1053 - 1053

Опубликована: Март 29, 2023

Plant-derived exosome-like nanoparticles (PDENs) comprise various bioactive biomolecules. As an alternative cell-free therapeutic approach, they have the potential to deliver nano-bioactive compounds human body, and thus lead anti-inflammatory, antioxidant, anti-tumor benefits. Moreover, it is known that Indonesia one of herbal centers world, with abundance unexplored sources PDENs. This encouraged further research in biomedical science develop natural richness plants as a source for welfare. study aims verify PDENs purposes, especially regenerative therapy applications, by collecting analyzing data from latest relevant developments.

Язык: Английский

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Current Knowledge and Future Perspectives of Exosomes as Nanocarriers in Diagnosis and Treatment of Diseases

International Journal of Nanomedicine, Год журнала: 2023, Номер Volume 18, С. 4751 - 4778

Опубликована: Авг. 1, 2023

Abstract: Exosomes, as natural nanocarriers, characterized with low immunogenicity, non-cytotoxicity and targeted delivery capability, which have advantages over synthetic nanocarriers. Recently, exosomes shown great potential diagnostic markers for diseases are also considered a promising cell-free therapy. Engineered significantly enhanced the efficacy precision of delivering therapeutic agents, currently being extensively employed in investigations various ailments, including oncology, inflammatory disorders, degenerative conditions. Particularly, engineered enable agent loading, modification, evasion MPS phagocytosis, intelligent control, bioimaging, been developed multifunctional nano-delivery platforms recent years. The utilization bioactive scaffolds that loaded exosome has to substantially augment retention, extend release, enhance efficacy. This approach advanced from conventional hydrogels nanocomposite hydrogels, nanofiber 3D printing, resulting superior physical biological properties effectively address limitations scaffolds. Additionally, plant-derived exosomes, can participate gut flora remodeling via oral administration, an ideal platform treatment intestinal diseases. Consequently, there is interest nanocarriers applications. comprehensive review provides overview biogenesis, composition, isolation methods exosomes. it examines pathological mechanisms diseases, tumors, Furthermore, this highlights significance microbial-derived Strategies specific applications scaffold-loaded further summarized, especially some new techniques such large-scale loading technique, macromolecular development nano-scaffold-loaded delivery. benefits using gut-related discussed. challenges, opportunities, prospects exosome-based disease diagnosis summarized both preclinical clinical viewpoints. Keywords: diagnosis,

Язык: Английский

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Plant-derived exosomes: a green approach for cancer drug delivery

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(9), С. 2236 - 2252

Опубликована: Янв. 1, 2024

Plant-derived exosomes (PDEs) show promising potential to be developed as a therapeutic agent against cancer, owing their multiple advantages such low toxicity, biocompatibility, availability, affordability, etc.

Язык: Английский

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Ginseng-derived nanoparticles alleviate inflammatory bowel disease via the TLR4/MAPK and p62/Nrf2/Keap1 pathways

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Фев. 1, 2024

Abstract Inflammatory bowel disease (IBD) is closely linked to the homeostasis of intestinal environment, and exosomes can be used treat IBD due their high biocompatibility ability effectively absorbed by tract. However, Ginseng-derived nanoparticles (GDNPs) have not been studied in this context mechanism action remains unclear. Here, we investigated GDNPs mediate intercellular communication a complex inflammatory microenvironment order IBD. We found that scavenge reactive oxygen species from immune cells epithelial cells, inhibit expression pro-inflammatory factors, promote proliferation differentiation stem as well enhancing diversity flora. significantly stabilise barrier thereby promoting tissue repair. Overall, proved ameliorate inflammation oxidative stress vivo vitro, acting on TLR4/MAPK p62/Keap1/Nrf2 pathways, exerting an anti-inflammatory antioxidant effect. mitigated mice reducing factors improving environment. This study offers new evidence potential therapeutic effects IBD, providing conceptual ground for alternative strategy. Graphical

Язык: Английский

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Nanoparticles for oral delivery: targeted therapy for inflammatory bowel disease

Journal of Materials Chemistry B, Год журнала: 2022, Номер 10(31), С. 5853 - 5872

Опубликована: Янв. 1, 2022

As a group of chronic and idiopathic gastrointestinal (GI) disorders, inflammatory bowel disease (IBD) is characterized by recurrent intestinal mucosal inflammation.

Язык: Английский

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Focusing on Future Applications and Current Challenges of Plant Derived Extracellular Vesicles

Pharmaceuticals, Год журнала: 2022, Номер 15(6), С. 708 - 708

Опубликована: Июнь 3, 2022

Plant derived extracellular vesicles (EVs) are nano-sized membranous released by plant cells, which contain lipids, proteins, nucleic acids and specific pharmacologically active substances. They safe, widely available expediently extractive. have gratifyingly biological activity against inflammation, cancer, bacteria oxidative aging, especially for the prevention or treatment of colitis, alcoholic liver, COVID-19. In addition, as natural drug carriers, EVs potential to target delivery small molecule drugs acid through oral, transdermal, injection. With above advantages, expected excellent strong competitiveness in clinical application preventive health care products future. We comprehensively reviewed latest separation methods physical characterization techniques EVs, summarized them disease a new carrier, analyzed prospect carrier Finally, problems hindering development at present consideration standardized discussed.

Язык: Английский

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Potential of Plant Exosome Vesicles from Grapefruit (Citrus × paradisi) and Tomato (Solanum lycopersicum) Juices as Functional Ingredients and Targeted Drug Delivery Vehicles

Antioxidants, Год журнала: 2023, Номер 12(4), С. 943 - 943

Опубликована: Апрель 17, 2023

Plant-derived extracellular vesicles (PEVs) have gained attention as promising bioactive nutraceutical molecules; their presence in common fruit juices has increased significance because human interaction is inevitable. The goal of this study was to evaluate the potential PEVs derived from grapefruit and tomato functional ingredients, antioxidant compounds, delivery vehicles. were isolated using differential ultracentrifugation found be similar size morphology mammalian exosomes. yield exosome-like (GEVs) higher than that (TEVs), despite latter having larger vesicle sizes. Furthermore, activity GEVs TEVs low comparison juice sources, indicating a limited contribution juice. showed efficiency being loaded with heat shock protein 70 (HSP70) TEVs, well TEV PEV-free HSP70 delivering glioma cells. Overall, our results revealed present ingredients they exert deliver molecules Although activity, role oxidative response cells should further addressed.

Язык: Английский

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Plant-derived nanovesicles: Further exploration of biomedical function and application potential

Acta Pharmaceutica Sinica B, Год журнала: 2023, Номер 13(8), С. 3300 - 3320

Опубликована: Март 7, 2023

Extracellular vesicles (EVs) are phospholipid bilayer actively secreted by cells, that contain a variety of functional nucleic acids, proteins, and lipids, important mediums intercellular communication. Based on their natural properties, EVs can not only retain the pharmacological effects source cells but also serve as delivery carriers. Among them, plant-derived nanovesicles (PNVs) characterized disease therapeutics with many advantages such simplicity, safety, eco-friendliness, low cost, toxicity due to abundant resources, large yield, risk immunogenicity in vivo. This review systematically introduces biogenesis, isolation methods, physical characterization, components PNVs, describes administration cellular uptake therapeutic agents. We highlight potential PNVs agents drug carriers, including anti-inflammatory, anticancer, wound healing, regeneration, antiaging properties well use treatment liver COVID-19. Finally, immunogenicity, current clinical application, possible challenges future development were analyzed. expect functions be further explored promote translation, thereby facilitating new framework for human diseases.

Язык: Английский

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Isolation of high-purity and high-stability exosomes from ginseng

Frontiers in Plant Science, Год журнала: 2023, Номер 13

Опубликована: Янв. 12, 2023

Exosomes are nano-sized extracellular vesicles that regulate cell growth and defense by delivering bioactive cellular constituents. They a promising material for biomedical cosmetic utilization, especially in medicinal crops such as ginseng. One main hurdle to their usage is the need method isolate stable exosomes with high purity. In this study, we first tested two methods from ginseng: ultracentrifugation, most widely used method; ExoQuick system, polymer-based exosome precipitation approach. We also designed third which combined ultracentrifugation methods. Size distribution analysis revealed isolation purity alone were 34.1% 59.7%, respectively, while combination greatly improved (83.3%). Furthermore, found increases colloidal stability of isolated ginseng exosomes, increase was almost double method. Lastly, showed can be high-purity high-stability model plant Arabidopsis. Overall, our findings indicate suitable plants including

Язык: Английский

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Edible plant-derived extracellular vesicles serve as promising therapeutic systems

Nano TransMed, Год журнала: 2023, Номер 2(2-3), С. 100004 - 100004

Опубликована: Сен. 1, 2023

Extracellular vesicles (EVs), which are natural nanocarriers characterized by a phospholipid bilayer structure, released living cells. They play crucial role in the intercellular transport of proteins, nucleic acids, lipids, and metabolites, facilitating substance delivery information exchange between In light recent numerous studies, EVs has been found to transcend their basic as mere vehicle. Instead, they demonstrate an impressive array biological activities, displaying preventive therapeutic potential mitigating various pathological processes encompassing cancer, neoplastic proliferation, infectious diseases, oxidative trauma. Particularly, derived from edible plants (EPDEVs) have emphasized for extensive range physiological regulatory functions animals humans, with targeted drug through oral administration. Leveraging these advantages, EPDEVs expected excellent competitiveness clinical applications or healthcare products. This review provides brief overview biogenesis, composition EPDEVs, summarizes mechanisms. It also analyzes methods isolating purifying plant-EVs, assessing advantages disadvantages; discusses latest advancements biomedical applications, concludes prospective insight into research development directions EPDEVs.

Язык: Английский

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