Photodermatology Photoimmunology & Photomedicine,
Год журнала:
2025,
Номер
41(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Objective
Exosomes
(Exos)
from
adipose
derived
stem
cells
(ADSCs)
can
delay
skin
photoaging,
but
their
effects
on
reactive
oxygen
species
(ROS)
remains
unclear.
This
study
aimed
to
investigate
the
relationship
between
cell
exosomes
(ADSCs‐Exos)
in
anti‐photoaging
of
and
glutathione
(GSH)/
ROS
expression
human
fibroblasts.
Methods
A
photoaging
model
was
established
by
irradiating
fibroblasts
with
ultraviolet
B
(UVB)
light
vitro.
Next,
ADSCs
were
isolated
for
treating
photoaged
Afterwards,
alterations
analyzed
a
series
assays
including
senescence‐associated
β‐galactosidase
(SA‐β‐Gal)
staining,
p16
expression,
GSH
content.
Results
After
fibroblast
subjected
ADSCs‐Exos
treatment,
we
found
that
high
concentration
exosome
group
had
highest
Cellular
staining
showed
levels
SA‐β‐Gal,
p16,
concentration‐treated
lower
than
other
groups.
Conclusions
protect
via
increasing
ratio
GSH/ROS.
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
This
review
highlights
recent
progress
in
exosome-based
drug
delivery
for
cancer
therapy,
covering
exosome
biogenesis,
cargo
selection
mechanisms,
and
their
application
across
multiple
types.
As
small
extracellular
vesicles,
exosomes
exhibit
high
biocompatibility
low
immunogenicity,
making
them
ideal
vehicles
capable
of
efficiently
targeting
cells,
minimizing
off-target
damage
side
effects.
aims
to
explore
the
potential
with
a
focus
on
applications
chemotherapy,
gene
immunomodulation.
Additionally,
challenges
related
production
standardization
are
analyzed,
highlighting
importance
addressing
these
issues
clinical
application.
In
conclusion,
systems
offer
promising
future
therapies.
Further
research
should
aim
enhance
efficiency
facilitate
translation,
paving
way
innovative
treatment
strategies.
Pharmaceutics,
Год журнала:
2024,
Номер
16(5), С. 674 - 674
Опубликована: Май 16, 2024
The
escalating
demand
for
enhanced
therapeutic
efficacy
and
reduced
adverse
effects
in
the
pharmaceutical
domain
has
catalyzed
a
new
frontier
of
innovation
research
field
pharmacy:
novel
drug
delivery
systems.
These
systems
are
designed
to
address
limitations
conventional
administration,
such
as
abbreviated
half-life,
inadequate
targeting,
low
solubility,
bioavailability.
As
disciplines
pharmacy,
materials
science,
biomedicine
continue
advance
converge,
development
efficient
safe
systems,
including
biopharmaceutical
formulations,
garnered
significant
attention
both
domestically
internationally.
This
article
presents
an
overview
latest
advancements
categorized
into
four
primary
areas:
carrier-based
coupling-based
targeted
intelligent
devices,
based
on
their
main
objectives
methodologies.
Additionally,
it
critically
analyzes
technological
bottlenecks,
current
challenges,
future
trends
application
Pharmaceutics,
Год журнала:
2024,
Номер
16(6), С. 709 - 709
Опубликована: Май 24, 2024
Of
all
the
numerous
nanosized
extracellular
vesicles
released
by
a
cell,
endosomal-originated
exosomes
are
increasingly
recognized
as
potential
therapeutics,
owing
to
their
inherent
stability,
low
immunogenicity,
and
targeted
delivery
capabilities.
This
review
critically
evaluates
transformative
of
exosome-based
modalities
across
pharmaceutical
precision
medicine
landscapes.
Because
precise
biomolecular
cargo
delivery,
posited
ideal
candidates
in
drug
enhancing
regenerative
strategies,
advancing
diagnostic
technologies.
Despite
significant
market
growth
projections
exosome
therapy,
its
utilization
is
encumbered
substantial
scientific
regulatory
challenges.
These
include
lack
universally
accepted
protocols
for
isolation
complexities
associated
with
navigating
environment,
particularly
guidelines
set
forth
U.S.
Food
Drug
Administration
(FDA).
presents
comprehensive
overview
current
research
trajectories
aimed
at
addressing
these
impediments
discusses
prospective
advancements
that
could
substantiate
clinical
translation
exosomal
therapies.
By
providing
analysis
both
capabilities
hurdles
therapeutic
applications,
this
article
aims
inform
direct
future
paradigms,
thereby
fostering
integration
systems
into
mainstream
practice.
ACS Omega,
Год журнала:
2023,
Номер
8(46), С. 43374 - 43387
Опубликована: Ноя. 9, 2023
Exosomes
are
nanoscale
vesicles
secreted
by
living
cells
that
have
similar
membrane
composition
to
parental
and
carry
a
variety
of
proteins,
lipids,
nucleic
acids.
Therefore,
exosomes
certain
biological
activities
play
an
important
role
in
intercellular
communication.
On
the
basis
its
potential
as
carrier
for
drug
delivery
systems,
been
engineered
compensate
shortage
natural
through
various
engineering
strategies
improving
efficiency,
enhancing
targeting
tissues
organs,
extending
circulating
half-life
exosomes.
This
review
focuses
on
loading
drugs
different
strategies,
discussions
exosome
surface
modification
summarizes
advantages
disadvantages
strategies.
In
addition,
this
provides
overview
recent
applications
number
refractory
relapsable
diseases.
has
provide
reference
further
research
development
Expert Opinion on Drug Delivery,
Год журнала:
2024,
Номер
21(1), С. 45 - 70
Опубликована: Янв. 2, 2024
Introduction
Exosomes,
a
type
of
extracellular
vesicles,
are
effective
tools
for
delivering
small-molecule
drugs
and
biological
therapeutics
into
cells
tissues.
Surface
modifications
with
targeting
ligands
ensure
precise
delivery
to
specific
cells,
minimizing
accumulation
in
healthy
organs
reducing
the
side
effects.
This
is
rapidly
growing
area
drug
research
this
review
aims
comprehensively
discuss
recent
advances
field.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Июнь 27, 2024
Exosomes,
as
a
class
of
small
extracellular
vesicles
closely
related
to
the
biological
behavior
various
types
tumors,
are
currently
attracting
research
attention
in
cancer
diagnosis
and
treatment.
Regarding
diagnosis,
stability
their
membrane
structure
wide
distribution
body
fluids
render
exosomes
promising
biomarkers.
It
is
expected
that
exosome-based
liquid
biopsy
will
become
an
important
tool
for
tumor
future.
For
treatment,
exosomes,
“golden
communicators”
between
cells,
can
be
designed
deliver
different
drugs,
aiming
achieve
low-toxicity
low-immunogenicity
targeted
delivery.
Signaling
pathways
exosome
contents
also
used
safer
more
effective
immunotherapy
against
tumors.
Exosomes
derived
from
range
sources,
exhibit
characteristics
well
clinical
application
advantages
therapies.
In
this
review,
we
analyzed
main
sources
have
great
potential
broad
prospects
therapy.
Moreover,
compared
therapeutic
advantages,
providing
new
ideas
exosomes.
