Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic‐Assisted Approaches Toward Smart Cancer Diagnosis and Analysis DOI
Seyyed Mojtaba Mousavi, Seyyed Alireza Hashemi, Masoomeh Yari Kalashgrani

и другие.

ChemMedChem, Год журнала: 2023, Номер 19(1)

Опубликована: Ноя. 2, 2023

Exosomes are essential indicators of molecular mechanisms involved in interacting with cancer cells and the tumor environment. As nanostructures based on lipids nucleic acids, exosomes provide a communication pathway for information transfer by transporting biomolecules from target cell to other cells. Importantly, these extracellular vesicles released into bloodstream most invasive cells, i. e., cells; this way, they could be considered promising specific biomarker diagnosis. In matter, CRISPR-Cas systems microfluidic approaches practical tools diagnosis understanding biology. systems, as genome editing approach, way inactivate or even remove gene without affecting intracellular mechanisms. These vital about factors development that lead more effective treatment. Meanwhile, can also significantly benefit research due their proper sensitivity, high throughput, low material consumption, cost, advanced spatial temporal control. Thereby, employing CRISPR-Cas- microfluidics-based toward exosome monitoring valuable source therapy This review assesses recent progress accurate in-depth study behavior.

Язык: Английский

Recent Advances in Extracellular Vesicles in Amyotrophic Lateral Sclerosis and Emergent Perspectives DOI Creative Commons
Gonçalo J. M. Afonso, Carla Cavaleiro, Jorge Valero

и другие.

Cells, Год журнала: 2023, Номер 12(13), С. 1763 - 1763

Опубликована: Июль 1, 2023

Amyotrophic lateral sclerosis (ALS) is a severe and incurable neurodegenerative disease characterized by the progressive death of motor neurons, leading to paralysis death. It rare high patient-to-patient heterogeneity, which makes its study arduous complex. Extracellular vesicles (EVs) have emerged as important players in development ALS. Thus, ALS phenotype-expressing cells can spread their abnormal bioactive cargo through secretion EVs, even distant tissues. Importantly, owing nature composition, EVs’ formation be exploited for better comprehension this elusive identification novel biomarkers, well potential therapeutic applications, such those based on stem cell-derived exosomes. This review highlights recent advances role EVs etiopathology how promising new strategies.

Язык: Английский

Процитировано

10
Membrane Vesicles as Drug Delivery Systems: Source, Preparation, Modification, Drug Loading, In Vivo Administration and Biodistribution, and Application in Various Diseases DOI Creative Commons

Chenhan Sun,

Ying Qin,

H.L. Zhuang

и другие.

Pharmaceutics, Год журнала: 2023, Номер 15(7), С. 1903 - 1903

Опубликована: Июль 7, 2023

Bioinspired (or biologically inspired) drug delivery systems (DDSs) have been intensively studied in the last decades. As bioinspired DDSs, membrane vesicles, including extracellular vesicles (EVs) released from eukaryotic cells, outer (OMVs) bacteria, cell-bound (CBMVs) isolated situ cell surfaces, reorganized after isolation of plasma and others rapidly developed are attracting more attention. Most recently, a collection 25 papers on advances vesicle-based was published Special Issue Pharmaceutics entitled “Advances systems”. These cover many related topics source, preparation, modification, loading, vivo administration biodistribution (mainly or exosomes bacterial vesicles), as well application DDSs treatment various diseases.

Язык: Английский

Процитировано

8
Exosomes for CRISPR-Cas9 Delivery: The Cutting Edge in Genome Editing DOI
Cynthia Aslan, Naime Majidi Zolbanin, Fatemeh Faraji

и другие.

Molecular Biotechnology, Год журнала: 2023, Номер 66(11), С. 3092 - 3116

Опубликована: Ноя. 27, 2023

Язык: Английский

Процитировано

8
Multifunctional extracellular vesicles and edaravone-loaded scaffolds for kidney tissue regeneration by activating GDNF/RET pathway DOI Creative Commons
Seung Yeon Lee, Jeong Min Park, Won‐Kyu Rhim

и другие.

Nano Convergence, Год журнала: 2024, Номер 11(1)

Опубликована: Окт. 26, 2024

With the severity of chronic kidney disease worldwide, strategies to recover renal function via tissue regeneration provide alternatives replacement therapy. To exclude side effects from direct cell transplantation, extracellular vesicles (EVs) are great substitutes representing paracrine signaling. build three-dimensional structures for implantation into 5/6 nephrectomy model by incorporating bioactive materials, including multifunctional EVs (mEVs), porous PMEZE/mEV scaffolds were developed in combination with edaravone (EDV; E) and mEV based on PMEZ PLGA (P), MH-RA (M), ECM (E), ZnO-ALA (Z). The oxygen free radical scavenger EDV was incorporated induce tubular regeneration. mEVs engineered serve regenerative activities a two SDF-1α overexpressed tonsil-derived mesenchymal stem cells (sEVs) intermediate mesoderm (IM) during differentiation progenitor (dEVs). displayed beneficial facilitating migration inducing surrounding cells, improved regulating GDNF/RET pathway their downstream genes. promotion MSC recruitment confirmed sEV particles number dependently, regulation effect its enhanced elucidated using vitro analysis. tubules additionally demonstrated through increased expression aquaporin-1 (AQP-1) cadherin-16 (CDH16) proximal tubules, calbindin PAX2 distal defect model. these, structural functional recovery achieved mice

Язык: Английский

Процитировано

2
Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic‐Assisted Approaches Toward Smart Cancer Diagnosis and Analysis DOI
Seyyed Mojtaba Mousavi, Seyyed Alireza Hashemi, Masoomeh Yari Kalashgrani

и другие.

ChemMedChem, Год журнала: 2023, Номер 19(1)

Опубликована: Ноя. 2, 2023

Exosomes are essential indicators of molecular mechanisms involved in interacting with cancer cells and the tumor environment. As nanostructures based on lipids nucleic acids, exosomes provide a communication pathway for information transfer by transporting biomolecules from target cell to other cells. Importantly, these extracellular vesicles released into bloodstream most invasive cells, i. e., cells; this way, they could be considered promising specific biomarker diagnosis. In matter, CRISPR-Cas systems microfluidic approaches practical tools diagnosis understanding biology. systems, as genome editing approach, way inactivate or even remove gene without affecting intracellular mechanisms. These vital about factors development that lead more effective treatment. Meanwhile, can also significantly benefit research due their proper sensitivity, high throughput, low material consumption, cost, advanced spatial temporal control. Thereby, employing CRISPR-Cas- microfluidics-based toward exosome monitoring valuable source therapy This review assesses recent progress accurate in-depth study behavior.

Язык: Английский

Процитировано

6