BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells
Dan Zhu,
Zongbin Sun,
Jiayun Wei
и другие.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 3475 - 3495
Опубликована: Апрель 1, 2024
Purpose:
Human
umbilical
cord
mesenchymal
stem
cell
(hucMSC)-derived
small
extracellular
vesicles
(sEVs)
are
natural
nanocarriers
with
promising
potential
in
treating
liver
fibrosis
and
have
widespread
applications
the
fields
of
nanomedicine
regenerative
medicine.
However,
therapeutic
efficacy
hucMSC-sEVs
is
currently
limited
owing
to
their
non-specific
distribution
vivo
partial
removal
by
mononuclear
macrophages
following
systemic
delivery.
Thus,
can
be
improved
through
development
engineered
capable
overcome
these
limitations.
Patients
Methods:
To
improve
anti-liver
hucMSC-sEVs,
we
genetically
overexpress
anti-fibrotic
gene
bone
morphogenic
protein
7
(
BMP7
)
parental
cells.
This
was
achieved
using
lentiviral
transfection,
which
BMP7-loaded
were
isolated
ultracentrifugation.
First,
induced
C57BL/6J
mice
intraperitoneal
injection
50%
carbon
tetrachloride
(CCL4)
twice
a
week
for
8
weeks.
These
subsequently
treated
BMP7+sEVs
via
tail
vein
injection,
effect
validated
animal
imaging,
immunohistochemistry
(IHC),
tissue
immunofluorescence,
enzyme-linked
immunosorbent
assay
(ELISA).
Finally,
function
studies
performed
confirm
results.
Results:
Liver
imaging
histopathology
confirmed
that
could
reach
aggregate
around
activated
hepatic
stellate
cells
(aHSCs)
significantly
stronger
compared
those
blank
or
negative
control-transfected
hucMSC-sEVs.
In
vitro,
promoted
phenotypic
reversal
aHSCs
inhibited
proliferation
enhance
effects.
Conclusion:
offer
novel
strategy
clinical
treatment
fibrosis.
Keywords:
hucMSCs,
sEVs,
aHSCs,
nanocarrier,
Язык: Английский
Frontier role of extracellular vesicles in kidney disease
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Сен. 20, 2024
Язык: Английский
Engineered ATP-Loaded Extracellular Vesicles Derived from Mesenchymal Stromal Cells: A Novel Strategy to Counteract Cell ATP Depletion in an In Vitro Model
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3424 - 3424
Опубликована: Апрель 5, 2025
The
use
of
adenosine
triphosphate
(ATP)
has
shown
promising
effects
in
alleviating
ischemic
damage
across
various
tissues.
However,
the
penetration
ATP
into
kidney
tubular
cells
presents
a
challenge
due
to
their
unique
anatomical
and
physiological
properties.
In
this
study,
we
introduce
novel
bioinspired
drug
delivery
system
utilizing
extracellular
vesicles
(EVs)
derived
from
mesenchymal
stromal
(MSCs)
engineered
carry
ATP.
ATP-loaded
liposomes
(ATP-LPs)
EVs
(ATP-EVs)
were
prepared
using
microfluidic
technology,
followed
by
characterization
morphology
(DLS,
NTA,
SEM,
TEM),
content,
release
rate
at
37
°C
(pH
7.4).
Additionally,
efficacy
ATP-LPs
ATP-EVs
was
evaluated
vitro
on
renal
(HK2
cells)
under
chemically
induced
ischemia.
results
indicated
successful
enrichment
EVs,
with
showing
no
significant
changes
or
size
compared
naïve
EVs.
Notably,
demonstrated
superior
retention
ATP-LPs,
protecting
degradation
environment.
an
ATP-depleted
HK2
cell
model,
only
effectively
restored
levels,
preserving
viability
reducing
apoptotic
gene
expression
(BCL2-BAX).
This
study
is
first
successfully
demonstrate
direct
as
carriers.
Язык: Английский
Extracellular Vesicles in Renal Inflammatory Diseases: Revealing Mechanisms of Extracellular Vesicle-Mediated Macrophage Regulation
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3646 - 3646
Опубликована: Апрель 12, 2025
Renal
inflammatory
diseases
are
a
group
of
severe
conditions
marked
by
significant
morbidity
and
mortality.
Extracellular
vesicles
(EVs),
as
facilitators
intercellular
communication,
have
been
recognized
pivotal
regulators
renal
diseases,
significantly
contributing
to
these
modulating
immune
responses
among
other
mechanisms.
This
review
highlights
the
intricate
mechanisms
through
which
EVs
modulate
macrophage-kidney
cell
interactions
regulating
macrophages,
principal
cells
within
milieu.
regulation
subsequently
influences
pathophysiology
such
acute
kidney
injury
chronic
disease.
Furthermore,
understanding
offers
novel
opportunities
alleviate
consequences
associated
with
diseases.
In
addition,
we
summarize
therapeutic
landscape
based
on
EV-mediated
macrophage
regulatory
mechanisms,
highlighting
potential
biomarkers
targets
well
challenges
limitations
translating
therapies
into
clinical
practice.
Язык: Английский
Engineered Extracellular Vesicles in Chronic Kidney Diseases: A Comprehensive Review
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 2377 - 2393
Опубликована: Март 1, 2024
Abstract:
Chronic
kidney
diseases
(CKD)
present
a
formidable
global
health
challenge,
characterized
by
deficiency
of
effective
treatment
options.
Extracellular
vesicles
(EVs),
recognized
as
multifunctional
drug
delivery
systems
in
biomedicine,
have
gained
accumulative
interest.
Specifically,
engineered
EVs
emerged
promising
therapeutic
approach
for
targeted
delivery,
potentially
addressing
the
complexities
CKD
management.
In
this
review,
we
systematically
dissect
EVs,
elucidating
their
classification,
biogenesis,
composition,
and
cargo
molecules.
Furthermore,
explore
techniques
EV
engineering
strategies
precise
renal
focusing
on
loading
transportation,
providing
comprehensive
perspective.
Moreover,
review
also
discusses
summarizes
diverse
applications
CKD,
emphasizing
anti-inflammatory,
immunomodulatory,
renoprotective,
tissue-regenerating
effects.
It
critically
evaluates
challenges
limitations
translating
therapies
from
laboratory
settings
to
clinical
applications,
while
outlining
future
prospects
emerging
trends.
Keywords:
extracellular
vesicles,
nanomaterials,
chronic
diseases,
systems,
application
Язык: Английский
A quantitative review of nanotechnology‐based therapeutics for kidney diseases
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology,
Год журнала:
2024,
Номер
16(2)
Опубликована: Март 1, 2024
Abstract
Kidney‐specific
nanocarriers
offer
a
targeted
approach
to
enhance
therapeutic
efficacy
and
reduce
off‐target
effects
in
renal
treatments.
The
can
achieve
organ
or
cell
specificity
via
passive
targeting
active
mechanisms.
Passive
capitalizes
on
the
unique
physiological
traits
of
kidney,
with
factors
like
particle
size,
charge,
shape,
material
properties
enhancing
specificity.
Active
targeting,
other
hand,
achieves
through
ligand‐receptor
interactions,
modifying
molecules,
peptides,
antibodies
for
receptor‐mediated
delivery.
Nanotechnology‐enabled
therapy
targets
diseased
kidney
tissue
by
modulating
podocytes
immune
cells
inflammation
repair,
inhibiting
myofibroblast
differentiation
mitigate
fibrosis.
This
review
summarizes
current
reports
drug
delivery
systems
that
have
been
tested
vivo,
identifies
may
preferentially
accumulate
quantitatively
compares
various
cargo‐carrier
combinations
outline
optimal
strategies
future
research
directions.
article
is
categorized
under:
Nanotechnology
Approaches
Biology
>
Nanoscale
Systems
Therapeutic
Drug
Discovery
Emerging
Technologies
Язык: Английский
State of the Art and New Trends from the Second International StemNet Meeting
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(4), С. 2221 - 2221
Опубликована: Фев. 13, 2024
The
Second
International
StemNet
(Federation
of
Stem
Cell
Research
Associations)
meeting
took
place
on
18–20
October
2023
in
Brescia
(Italy),
with
the
support
University
and
Zooprophylactic
Institute
Lombardy
Emilia
Romagna.
program
was
articulated
nine
sections:
(1)
Biomedical
Communication
Italy:
Critical
Aspects;
(2)
Next
Generation
Session;
(3)
Cell-Free
Therapies;
(4)
Tips
Tricks
Valorisation;
(5)
Cells
Cancer;
(6)
Veterinary
Applications;
(7)
Clinical
(8)
Organoids
3D
Systems;
(9)
induced
pluripotent
stem
cells
(iPCS)
Gene
Therapy.
National
speakers
presented
their
scientific
works,
inspiring
debates
discussions
among
attendees.
participation
high,
especially
because
young
researchers
who
animated
all
sessions
rich
poster
session.
Язык: Английский
New approaches to acute kidney injury
Clinical Kidney Journal,
Год журнала:
2024,
Номер
17(Supplement_2), С. 65 - 81
Опубликована: Ноя. 22, 2024
ABSTRACT
Acute
kidney
injury
(AKI)
is
a
common
and
serious
clinical
syndrome
that
involves
complex
interplay
between
different
cellular,
molecular,
metabolic
immunologic
mechanisms.
Elucidating
these
pathophysiologic
mechanisms
crucial
to
identify
novel
biomarkers
therapies.
Recent
innovative
methodologies
the
advancement
of
existing
technologies
has
accelerated
our
understanding
AKI
led
unexpected
new
therapeutic
candidates.
The
aim
this
review
introduce
update
reader
about
recent
developments
applying
in
omics,
imaging,
nanomedicine
artificial
intelligence
research,
plus
provide
examples
where
can
be
translated
improve
patient
care.
Язык: Английский