Internalization of transferrin-tagged Myxococcus xanthus encapsulins into mesenchymal stem cells DOI Creative Commons
А. Н. Габашвили, Natalya Alexandrushkina, Elizaveta N. Mochalova

и другие.

Experimental Biology and Medicine, Год журнала: 2024, Номер 249

Опубликована: Май 7, 2024

Currently, various functionalized nanocarrier systems are extensively studied for targeted delivery of drugs, peptides, and nucleic acids. Joining the approaches genetic chemical engineering may produce novel carriers precise targeting different cellular proteins, which is important both therapy diagnosis pathologies. Here we present nanocontainers based on vectorized genetically encoded Myxococcus xanthus (Mx) encapsulin, confining a fluorescent photoactivatable mCherry (PAmCherry) protein. The shells such encapsulins were modified using conjugation human transferrin (Tf) prelabeled with fluorescein-6 (FAM) maleimide acting as vector. We demonstrate that encapsulin specifically binds to receptors (TfRs) membranes mesenchymal stromal/stem cells (MSCs) followed by internalization into cells. Two spectrally separated signals from Tf-FAM PAmCherry clearly distinguishable co-localized. It shown Tf-tagged Mx internalized MSCs much more efficiently than fibroblasts. has been also found unlabeled Tf effectively competes conjugated Mx-Tf-FAM formulations. That indicates conjugate Tf-TfR endocytosis pathway. developed nanoplatform can be used an alternative conventional nanocarriers of, e.g., material MSCs.

Язык: Английский

Advancing glioblastoma treatment through iron metabolism: A focus on TfR1 and Ferroptosis innovations DOI
Matías Daniel Caverzán, Luis Exequiel Ibarra

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 278, С. 134777 - 134777

Опубликована: Авг. 15, 2024

Язык: Английский

Процитировано

6
Efficient Chlorostannate Modification of Magnetite Nanoparticles for Their Biofunctionalization DOI Open Access
Maria O. Zolotova, Sergey L. Znoyko, А. В. Орлов

и другие.

Materials, Год журнала: 2024, Номер 17(2), С. 349 - 349

Опубликована: Янв. 10, 2024

Magnetite nanoparticles (MNPs) are highly favored materials for a wide range of applications, from smart composite and biosensors to targeted drug delivery. These multifunctional applications typically require the biofunctional coating MNPs that involves various conjugation techniques form stable MNP–biomolecule complexes. In this study, cost-effective method is developed chlorostannate modification MNP surfaces provides efficient one-step with biomolecules. The proposed was validated using obtained via an optimized co-precipitation technique included use degassed water, argon atmosphere, pre-filtering FeCl2 FeCl3 solutions followed by surface stannous chloride. resulting chlorostannated were comprehensively characterized, their efficiency compared both carboxylate-modified unmodified MNPs. biorecognition performance verified magnetic immunochromatography. Mouse monoclonal antibodies folic acid served as model biomolecules conjugated produce nanobioconjugates, while acid–gelatin conjugates immobilized on test lines immunochromatography lateral flow strips. specific trapping nanobioconjugates antibody–antigen interactions registered sensitive particle quantification technique. versatile, rapid, convenient tool creating span in vitro diagnostics, separation, potential vivo uses.

Язык: Английский

Процитировано

2
Nanotechnology in Glioblastoma Therapy: Advances in Drug Delivery Systems and Diagnostic Approaches DOI Creative Commons

Maimoona Qindeel,

Mahnoor Irfan,

Sami Ullah

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 102, С. 106322 - 106322

Опубликована: Окт. 24, 2024

Язык: Английский

Процитировано

1
Internalization of transferrin-tagged Myxococcus xanthus encapsulins into mesenchymal stem cells DOI Creative Commons
А. Н. Габашвили, Natalya Alexandrushkina, Elizaveta N. Mochalova

и другие.

Experimental Biology and Medicine, Год журнала: 2024, Номер 249

Опубликована: Май 7, 2024

Currently, various functionalized nanocarrier systems are extensively studied for targeted delivery of drugs, peptides, and nucleic acids. Joining the approaches genetic chemical engineering may produce novel carriers precise targeting different cellular proteins, which is important both therapy diagnosis pathologies. Here we present nanocontainers based on vectorized genetically encoded Myxococcus xanthus (Mx) encapsulin, confining a fluorescent photoactivatable mCherry (PAmCherry) protein. The shells such encapsulins were modified using conjugation human transferrin (Tf) prelabeled with fluorescein-6 (FAM) maleimide acting as vector. We demonstrate that encapsulin specifically binds to receptors (TfRs) membranes mesenchymal stromal/stem cells (MSCs) followed by internalization into cells. Two spectrally separated signals from Tf-FAM PAmCherry clearly distinguishable co-localized. It shown Tf-tagged Mx internalized MSCs much more efficiently than fibroblasts. has been also found unlabeled Tf effectively competes conjugated Mx-Tf-FAM formulations. That indicates conjugate Tf-TfR endocytosis pathway. developed nanoplatform can be used an alternative conventional nanocarriers of, e.g., material MSCs.

Язык: Английский

Процитировано

0