Plant-Derived Extracellular Vesicles: Natural Nanocarriers for Biotechnological Drugs
Processes,
Год журнала:
2024,
Номер
12(12), С. 2938 - 2938
Опубликована: Дек. 23, 2024
Plant-derived
extracellular
vesicles
(PDEVs)
are
lipid
bilayer
nanoparticles,
naturally
produced
by
plant
cells,
with
sizes
ranging
from
50
to
500
nm.
Recent
studies
have
highlighted
their
great
potential
in
the
biotechnological
and
medical
fields,
due
natural
origin,
high
biocompatibility
intrinsic
therapeutic
properties.
PDEVs
contain
a
complex
biological
cargo
of
proteins,
lipids,
nucleic
acids
secondary
metabolites,
including
antioxidants
anti-inflammatory
molecules,
making
them
ideal
for
biomedical
applications
such
as
drug
delivery.
These
play
key
role
intercellular
communication
gene
regulation,
proving
be
particularly
promising
personalized
medicine.
ability
improve
stability
bioavailability,
optimizing
targeted
release
minimizing
side
effects.
Despite
some
challenges,
compositional
variability
need
standardized
protocols,
at
gunsight
innovative
research
aimed
improving
loading
capacity
specificity.
This
review
aims
provide
comprehensive
overview
PDEVs,
exploring
structure,
isolation
methods,
functional
characteristics,
applications,
highlighting
advantages
over
synthetic
nanoparticles
animal-derived
vesicles,
leading
an
sustainable
solution
development
new
approaches.
Язык: Английский
Engineering Strategies of Plant-Derived Exosome-Like Nanovesicles: Current Knowledge and Future Perspectives
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 12793 - 12815
Опубликована: Ноя. 1, 2024
Plant-derived
exosome-like
nanovesicles
(PELNs)
from
edible
plants,
isolated
by
ultracentrifugation,
size
exclusion
chromatography
or
other
methods,
were
proved
to
contain
a
variety
of
biologically
active
and
therapeutically
specific
components.
Recently,
investigations
in
the
field
PELN-based
biomedicine
have
been
conducted,
which
positioned
those
as
promising
tools
for
prevention
treatment
several
diseases,
with
their
natural
origin
potentially
offering
superior
biocompatibility
bioavailability.
However,
inadequate
targeting
limited
therapeutic
effects
constrain
utility
clinical
translation
PELNs.
Thus,
strategies
aiming
at
bridging
gap
engineering
PELNs
great
interest.
Those
approaches
include
membrane
hybridization,
physical
chemical
surface
functionalization
encapsulation
payloads.
Herein,
we
provide
comprehensive
overview
biogenesis
composition,
isolation
purification
methods
characterization
PELNs,
well
functions.
Current
knowledge
on
construction
biomedical
application
engineered
reviewed.
Additionally,
future
directions
perspectives
this
discussed
order
further
enrich
expand
prospects
Язык: Английский
Extracellular Vesicles and PlantCrystals for Improved Bioavailability of Curcumin as a BCS Class IV Drug
Molecules,
Год журнала:
2024,
Номер
29(24), С. 5926 - 5926
Опубликована: Дек. 16, 2024
The
limited
water
solubility
of
active
compounds
remains
a
significant
challenge
for
efficient
dermal
drug
delivery,
particularly
BCS
class
IV
drugs
such
as
curcumin.
This
study
aimed
to
enhance
curcumin’s
penetration
using
two
strategies:
extracellular
vesicles
(EVs)
and
plantCrystals
derived
from
soybeans.
EVs
were
isolated
classical
methods.
However,
containing
(PCEVs)
formed
during
the
preparation
through
bead
milling.
Curcumin
was
either
added
after
PCEVs
formed,
resulting
in
curcumin-added
PCEVs,
or
soybean
dispersion
before
milling,
forming
curcumin-loaded
PCEVs.
formulations
characterized
their
physicochemical
properties
assessed
efficacy
quantitative
dermatokinetic
semi-quantitative
ex
vivo
porcine
ear
models.
results
indicated
that
achieved
higher
compared
EVs,
with
approximately
1.5-fold
2.7-fold
increases
efficacy,
respectively.
Additionally,
showed
superior
depth,
while
curcumin
remained
stratum
corneum.
These
findings
suggest
strategy
via
milling
offers
more
effective
approach
than
improving
topical
delivery
like
Язык: Английский