CRISPR-Cas9 genome editing in crop breeding for climate change resilience: Implications for smallholder farmers in Africa

Journal of Agriculture and Food Research, Год журнала: 2024, Номер 16, С. 101132 - 101132

Опубликована: Март 28, 2024

Food insecurity and malnutrition, compounded by climate change, are seriously threatening the growing African population. Unpredictable precipitation patterns droughts contributing to declining crop productivity. Efforts increase agricultural productivity include adoption of crops that resistant change engaging in resilient agriculture. Currently, CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats - associated protein) technology is being used breeding practices improve traits such as drought tolerance, nutrition disease resistance. The aim this review explore use developing for mitigation food hunger attendant implications small holder farmers Africa. A comprehensive systematic search peer reviewed articles three bibliographic databases, PubMed, Web Science Google Scholar, was conducted identify documents relevant applications CRISPR-mediated genome editing improvement context. literature shows CRISPR-Cas has been harnessed enhance resilience nutritional content various crops, combatting biotic abiotic stresses. CRISPR-assisted strategies can help smallholder low-middle income countries Africa adapt without loss. By harnessing technology, benefit from with improved yields stress resistance thereby security sustainable agriculture on continent.

Язык: Английский

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Unveiling the therapeutic and nutritious potential of Vigna unguiculata in line with its phytochemistry

Heliyon, Год журнала: 2024, Номер 10(18), С. e37911 - e37911

Опубликована: Сен. 1, 2024

Язык: Английский

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Underutilized crops for diverse, resilient and healthy agri-food systems: a systematic review of sub-Saharan Africa

Frontiers in Sustainable Food Systems, Год журнала: 2024, Номер 8

Опубликована: Ноя. 28, 2024

Sub-Saharan Africa (SSA) faces increasing water scarcity, food and nutrition insecurity, poverty inequality under climate change. Under these circumstances, promoting locally adapted nutrient-dense crops is touted as a plausible adaptation strategy. We reviewed the utility of neglected underutilized crop species (NUS) change strategy to diversify local systems diets improve nutritional health environmental outcomes in SSA. conducted systematic literature review using Web Science Scopus research databases. Of 1,545 studies retrieved, 75 were included following multi-level screening process on Covidence guided by Preferred Reporting Items for Systematic reviews Meta-Analyses (PRISMA) guidelines. The consolidates fragmented knowledge application NUS different contexts. Despite growing interest, remain gendered stigmatized crops, marginalized research, development, marketing efforts lack explicit support from policy decision-makers. rhetoric purporting them, there worrying rise policies regulations that inadvertently hinder development reinforce dependence narrow basket security, undermining sovereignty. Some have received recognition their potential past decade. However, this neither universal nor systematic, which makes scaling up necessary but challenging. Consequently, progress mainstreaming continues lag. challenges, sub-Saharan Africa's better-bet option diversifying transitioning them be equitable, inclusive, resilient healthy; hence, provide positive people planet

Язык: Английский

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Secondary metabolites in selected cowpea [Vigna unguiculata (L.) Walp.] lines mediated resistance to flower thrips (Megalurothrips sjostedti Trybom)

Discover Plants., Год журнала: 2025, Номер 2(1)

Опубликована: Фев. 17, 2025

Flower thrips (Megalurothrips sjostedti Trybom) are a major pest of cowpea worldwide. Although some varieties exhibit resistance to these pests, the underlying mechanisms this remain unclear. This study aimed explore biochemical factors contributing against and measure levels specific compounds in resistant varieties. Six cultivars were tested using randomized complete block design across three field trials. At 44 days after planting, foliage flowers from each variety collected for laboratory analysis. Samples ten randomly chosen plants combined total protein, soluble amino acids, phenols, flavonoids, antioxidants. Data analysis revealed significant differences among lines. Most metabolites, except showed considerable variation Notably, lines TVu1509, Sanzi, TVNu1249 exhibited highest protein. Principal component indicated that first components explained about 83.59% variation, with contributions 36%, 28%, 19%, respectively. In conclusion, certain flower is associated higher

Язык: Английский

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Combining the CowPEAsy Web Application With in Planta Agroinfiltration for Native Promoter Validation in Vigna unguiculata

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

ABSTRACT Cowpea ( Vigna unguiculata ) is an important protein source in Sub‐Saharan Africa. Optimizing resilience and productivity through genetic engineering cowpea has been slow due part to a lack of defined species‐specific regulatory elements difficulty testing gene function within the native system. In many plant species, Agrobacterium ‐mediated transient expression widely used validate constructs before investing transgenic lines, but its implementation legumes challenging. this study, we optimized planta agroinfiltration assay trifoliate leaves using betalain reporter. To demonstrate “intact plant” aspect system, characterize drought‐inducible promoters by challenging plants with drought stress. Subsequently, identify broaden pool known cowpea, developed user‐friendly web application, CowPEAsy, allowing users interrogate from our canopy‐level, developmental‐series RNA‐Seq data set. Finally, identified six that showed constitutive across all conditions verified these This work provides vivo platform for preliminary validation other enhances current resources identifying suite physiologically relevant varying strengths.

Язык: Английский

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SILÍCIO COMO AGENTE MITIGADOR DA RESTRIÇÃO HÍDRICA EM FEIJÃO-CAUPI: AVALIAÇÃO DE PARÂMETROS DE CRESCIMENTO

Journal of Education Science and Health, Год журнала: 2025, Номер 5(1), С. 1 - 11

Опубликована: Фев. 15, 2025

O Vigna unguiculata é uma leguminosa amplamente cultivada devido ao seu elevado valor nutricional e econômico. Embora possua adaptações para suportar condições adversas, esta planta pode sofrer diversas alterações negativas quando submetida estresse abiótico, especialmente hídrico. Por esse motivo, essencial buscar tecnologias que viabilizem o cultivo dessa cultura sob tais adversidades. Nesse sentido, objetivou-se avaliar a eficácia da pulverização foliar de silício, na mitigação dos danos causados pelo hídrico em feijão-caupi, focando nos parâmetros crescimento planta. Para tanto, foi conduzido um experimento estufa utilizando-se delineamento inteiramente casualizado com quatro repetições, sendo avaliadas plantas sem restrição hídrica dois tratamentos, sendo, Controle (Nada Aplicado) silício (300 mg/L Si). Foram avaliados, taxa relativo parte aérea (TCRA, cm cm-1 dia-1), Taxa caulinar (TCRC, mm mm-1 Razão área (RAF, cm² g-1), massa (RMF, g g-1) específica (AFE, mg mm2). Os dados foram submetidos à análise variância testes comparações médias. Conclui-se que, reduziu do feijão-caupi nas variáveis TCRA, TCRC, RMF AFE, mas não afetou RAF. A suplementação melhorou maioria das estudadas, sugerindo beneficiar No entanto, mais pesquisas são necessárias entender completamente os efeitos como agente mitigador.

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Comparative Analysis of Five African Traditional Multipurpose Crops Using a Food Systems Approach

Food Reviews International, Год журнала: 2025, Номер unknown, С. 1 - 37

Опубликована: Апрель 7, 2025

Язык: Английский

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Controlled Environment Ecosystem: A Cutting-Edge Technology in Speed Breeding

ACS Omega, Год журнала: 2024, Номер 9(27), С. 29114 - 29138

Опубликована: Июнь 26, 2024

The controlled environment ecosystem is a meticulously designed plant growing chamber utilized for cultivating biofortified crops and microgreens, addressing hidden hunger malnutrition prevalent in the population. integration of speed breeding within such environments effectively eradicates morphological disruptions encountered traditional methods as inbreeding depression, male sterility, self-incompatibility, embryo abortion, other unsuccessful attempts. In contrast to unpredictable climate conditions that often prolong cycles 10-15 years 4-5 transgenic open ecosystems, techniques expedite achievement objectives F1-F6 generations 2-3 under conditions. comparison, may take 5-10 population line creation, 3-5 field trials, 1-2 variety release. effectiveness trait improvement development varies across different crops, requiring approximately 4 rice groundnut, 5 soybean, pea, oat, 6 sorghum,

Язык: Английский

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Cowpea Constraints and Breeding in Europe

Plants, Год журнала: 2023, Номер 12(6), С. 1339 - 1339

Опубликована: Март 16, 2023

Cowpea (Vigna unguiculata (L.) Walp.) is a legume with constant rate of cultivation in Southern European countries. Consumer demand for cowpea worldwide rising due to its nutritional content, while Europe constantly attempting reduce the deficit production pulses and invest new, healthy food market products. Although climatic conditions that prevail are not so harsh terms heat drought as tropical climates where mainly cultivated, confronts plethora abiotic biotic stresses yield-limiting factors In this paper, we summarize main constraints breeding methods have been or can be used. A special mention made availability plant genetic resources (PGRs) their potential purposes, aiming promote more sustainable cropping systems shifts become frequent fiercer, environmental degradation expands worldwide.

Язык: Английский

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Phenomenon of Non-Grain Production of Cultivated Land Has Become Increasingly Prominent over the Last 20 Years: Evidence from Guanzhong Plain, China

Agriculture, Год журнала: 2022, Номер 12(10), С. 1654 - 1654

Опубликована: Окт. 9, 2022

Cultivated land is the carrier of food production. As a populous country that regards cultivated as most valuable strategic resource, China has faced challenge phenomenon non-grain production (NGP) in recent years, which attracted great attention from Chinese government. Based on and grain data at plot scale 2000, 2010, 2019, this study explored evolutionary characteristics spatial pattern NGP Guanzhong Plain Shaanxi Province 2000 to 2019 with Geographic Information System analysis. The found clear trend expansion past 20 years. In 54 counties districts Guanzhong, there were different degrees NGP, showing an annual trend. agglomeration effect was significant. This important scientific value understanding patterns provides basis for formulation management policies.

Язык: Английский

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