CRISPR/Cas: a powerful tool for designing and improving oil crops
Trends in biotechnology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Genome-wide analysis of the calmodulin-binding transcription activator (CAMTA) gene family in Sesamum indicum L., and its role in abiotic stress tolerance traits
Plant Stress,
Год журнала:
2024,
Номер
13, С. 100532 - 100532
Опубликована: Июль 11, 2024
Calmodulin-binding
transcription
activator
(CAMTA)
is
one
of
the
key
factor
families
possessing
calcium
receptors
(calmodulins,
CaM).
It
modulates
expression
levels
genes
associated
with
ontogeny
and
various
biotic
abiotic
stress
factors.
The
CAMTA
family
were
known
to
be
involved
in
different
several
crop
species.
However,
their
functional
relevance
sesame
remains
unexplored.
To
understand
role
tolerance
sesame,
we
performed
a
genome-wide
analysis
identify
members
SiCAMTA
gene
family.
We
have
identified
reported
here
five
localized
on
four
chromosomes
within
genome.
In
silico
putative
2-kilobase
(kb)
promoter
regions
for
these
showed
that
phytohormone
response-related
cis-elements
predominated
SiCAMTA2
SiCAMTA5.
Also,
studied
its
modulated
levels,
special
reference
drought
waterlogging
stress.
revealed
SiCAMTA5
most
responsive
target
prediction
network
suggested
SiCAMTAs
could
bind
CGCG
cis
element
promoters
predicted
1202
genome,
including
stress-responsive
viz.
LEA,
PIP1–2,
PPO1,
SAP,
ARF17,
GA3OX1.
These
findings
validated
using
qPCR
10
establish
foundation
future
research
towards
tolerance.
Язык: Английский
Sculpting the Harvest: Genomics and Genome Editing Applications for Enhanced Oil Crop Development
Опубликована: Янв. 1, 2025
Язык: Английский
Genome-wide characterization of the soybean DOMAIN OF UNKNOWN FUNCTION 679 membrane protein gene family highlights their potential involvement in growth and stress response
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Сен. 8, 2023
The
DMP
(DUF679
membrane
proteins)
family
is
a
plant-specific
gene
that
encodes
proteins.
genes
are
suggested
to
be
involved
in
various
programmed
cell
death
processes
and
gamete
fusion
during
double
fertilization
Arabidopsis.
However,
their
functional
relevance
other
crops
remains
unknown.
This
study
identified
14
from
the
soybean
(
Glycine
max
)
characterized
physiochemical
properties,
subcellular
location,
structure,
promoter
regions
using
bioinformatics
tools.
Additionally,
tissue-specific
stress-responsive
expressions
were
analyzed
publicly
available
transcriptome
data.
Phylogenetic
analysis
of
198
s
monocots
dicots
revealed
six
clades,
with
clade-I
encoding
senescence-related
AtDMP1/2
orthologues
clade-II
including
pollen-specific
AtDMP8/9
orthologues.
largest
clade,
clade-III,
predominantly
included
monocot
s,
while
monocot-
dicot-specific
assembled
clade-IV
clade-VI,
respectively.
Evolutionary
suggests
GmDMP
underwent
purifying
selection
evolution.
Using
68
datasets,
expression
profiling
diverse
tissues
distinct
responses
abiotic
biotic
stresses.
Glyma.09G237500
Glyma.18G098300
showed
pistil-abundant
by
qPCR,
suggesting
they
could
potential
targets
for
female
organ-mediated
haploid
induction.
Furthermore,
cis-acting
regulatory
elements
primarily
related
stress-,
hormone-,
light-induced
pathways
regulate
which
consistent
divergent
involvement
growth
stress
responses.
Overall,
our
provides
comprehensive
report
on
framework
further
biological
or
crops.
Язык: Английский
Double‐digest restriction‐associated DNA sequencing‐based genotyping and its applications in sesame germplasm management
The Plant Genome,
Год журнала:
2024,
Номер
17(2)
Опубликована: Апрель 17, 2024
Sesame
(Sesamum
indicum
L.)
is
an
ancient
oilseed
crop
belonging
to
the
family
Pedaliaceae
and
a
globally
cultivated
for
its
use
as
oil
food.
In
this
study,
2496
sesame
accessions,
being
conserved
at
National
Genebank
of
ICAR-National
Bureau
Plant
Genetic
Resources
(NBPGR),
were
genotyped
using
genomics-assisted
double-digest
restriction-associated
DNA
sequencing
(ddRAD-seq)
approach.
A
total
64,910
filtered
single-nucleotide
polymorphisms
(SNPs)
utilized
assess
genome-scale
diversity.
Applications
information
(reduced
representation
restriction
enzymes)
are
demonstrated
through
development
molecular
core
collection
(CC)
representing
maximal
SNP
This
also
applied
in
developing
mid-density
panel
(MDP)
comprising
2515
hyper-variable
SNPs,
almost
equally
genic
non-genic
regions.
The
CC
384
representative
set
accessions
with
diversity,
was
identified
multiple
criteria
such
k-mer
(subsequence
length
"k"
sequence
read)
observed
heterozygosity,
CoreHunter3,
GenoCore,
genetic
differentiation.
coreset
constituted
around
15%
studied,
small
subset
had
captured
>60%
diversity
entire
population.
coreset,
admixture
analysis
shows
reduced
complexity,
increased
nucleotide
(π),
geographically
distributed
without
any
repetitiveness
germplasm.
Within
CC,
India-originated
exhibit
higher
(as
expected
based
on
center
concept),
than
those
that
procured
from
various
other
countries.
MDP
will
be
valuable
resource
accelerated
improvement
program.
Язык: Английский
Evaluation and validation of suitable reference genes for quantitative real-time PCR analysis in lotus (Nelumbo nucifera Gaertn.)
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Май 13, 2024
Abstract
The
qRT-PCR
technique
has
been
regarded
as
an
important
tool
for
assessing
gene
expression
diversity.
Selection
of
appropriate
reference
genes
is
essential
validating
deviation
and
obtaining
reliable
accurate
results.
Lotus
(
Nelumbo
nucifera
Gaertn)
a
common
aquatic
plant
with
aesthetic,
commercial,
cultural
values.
Twelve
candidate
genes,
which
are
typically
used
in
other
plants,
were
selected
this
study.
These
cloned
with,
specific
primers
designed
based
on
published
sequences.
In
particular,
the
level
each
was
examined
different
tissues
growth
stages
Lotus.
Notably,
stability
these
assessed
using
software
programs
geNorm
NormFinder.
As
result,
most
efficient
rootstock
expansion
TBP
UBQ.
addition,
EF-1α
various
floral
tissues,
while
ACT
GAPDH
stable
at
all
developmental
seed.
CYP
best
leaf
development,
but
TUA
least
stable.
Meanwhile,
profile
NnEXPA
analyzed
to
confirm
validity
findings.
It
concluded
that,
identified
genes.
results
study
may
help
researchers
select
thus
obtain
credible
further
quantitative
RT-qPCR
analyses
Язык: Английский
Molecular Diversity Studies and Core Development in Sesame Germplasm (Sesamum indicum L.) Using SSR Markers
Plant Molecular Biology Reporter,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 23, 2024
Язык: Английский
Unraveling the Amino Acid Synthesis in Maturity Sesame Seeds Based on the Integrative Analysis of Transcriptome and Metabolome
Food Frontiers,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 13, 2024
ABSTRACT
Sesame
plays
a
vital
role
in
food
industry
due
to
its
high
oil
yield,
antioxidant
potential,
and
substantial
protein
content.
Notably,
there
are
significant
differences
amino
acid
composition
sesame
seeds
at
various
developmental
stages.
However,
the
molecular
basis
regulatory
mechanism
underlying
production
largely
remain
unexplored.
To
unravel
these
mechanisms,
we
analyzed
metabolome
transcriptome
profiles
of
variety
across
four
distinct
growth
stages
(S1–S4).
Our
analysis
identified
total
17
acids,
with
glutamic
(Glu),
arginine
(Arg),
proline
(Pro),
tyrosine
(Tyr)
exhibiting
significantly
higher
abundances
mature
This
increased
abundance
correlated
elevated
expression
genes
involved
synthesis
genes.
Using
weighted
gene
co‐expression
network
analysis,
discovered
modules
associated
glutathione
metabolism,
biosynthesis,
proline,
synthesis,
along
candidate
that
regulate
metabolism.
differential
within
pathways
resulted
variations
contents
Glu,
Arg,
Pro,
Tyr
stage
(28
days
after
flowering,
S4)
compared
other
Correlation
revealed
strong
association
among
enzymes
glutamine
synthetase
(GS),
glutamate/aspartate–prephenate
aminotransferase
(PAT),
polyamine
oxidase
(PAO)
suggesting
their
potential
synthesis.
findings
provide
novel
insights
into
accumulation
acids
during
seeds,
highlighting
key
metabolic
this
process.
study
lays
groundwork
for
future
studies
aiming
enhance
nutritional
quality
yield
varieties.
Язык: Английский
Advances in Single-Cell Sequencing Technology and Its Application in Poultry Science
Genes,
Год журнала:
2022,
Номер
13(12), С. 2211 - 2211
Опубликована: Ноя. 25, 2022
Single-cell
sequencing
(SCS)
uses
a
single
cell
as
the
research
material
and
involves
three
dimensions:
genes,
phenotypes
biological
mechanisms.
This
type
of
can
locate
target
cells,
analyze
dynamic
changes
in
cells
relationships
between
pinpoint
molecular
mechanism
formation.
Currently,
common
problem
faced
by
animal
husbandry
scientists
is
how
to
apply
existing
science
technology
promote
production
high-quality
livestock
poultry
products
breed
for
disease
resistance;
this
also
bottleneck
sustainable
development
husbandry.
In
recent
years,
although
SCS
has
been
successfully
applied
fields
medicine
bioscience,
its
application
rarely
reported.
With
industry,
great
potential
(or
husbandry).
Therefore,
it
necessary
review
innovation
science.
article
summarizes
current
main
technical
methods
poultry,
which
provide
references
future
applications
precision
breeding,
prevention
control,
immunity,
identification.
Язык: Английский