Microorganisms, Год журнала: 2024, Номер 12(12), С. 2546 - 2546
Опубликована: Дек. 11, 2024
The phyllosphere (aerial plant surfaces colonized by microorganisms) remains an understudied ecosystem in terms of bacterial biogeography, particularly at intermediate or local spatial scales. This study characterized the community on leaves 87
Язык: Английский
BMC Plant Biology, Год журнала: 2024, Номер 24(1)
Опубликована: Июнь 17, 2024
Abstract Background Plants can retain atmospheric particulate matter (PM) through their unique foliar microstructures, which has a profound impact on the phyllosphere microbial communities. Yet, underlying mechanisms linking retention by microstructures to variations in communities remain mystery. In this study, we conducted field experiment with ten Ulmus lines. A series of analytical techniques, including scanning electron microscopy, atomic force and high-throughput amplicon sequencing, were applied examine relationship between surface PM retention, diversity L. Results We characterized leaf across Chun exhibited highly undulated abaxial dense stomatal distribution. Langya Xingshan possessed trichomes, while Lieye, Zuiweng, Daguo had sparsely distributed, short trichomes. Duomai, Qingyun, Lang sparse stomata flat surfaces, whereas Jinye distributed but extensive stomata. The mean values for total suspended (TSP), 2.5 , 2.5-10 10-100 > 100 135.76, 6.60, 20.10, 90.98, 13.08 µg·cm − 2 respectively. Trichomes substantially contributed larger undulations enhanced as evidenced positive correlations trichome density adaxial raw microroughness values. Phyllosphere patterns varied among lines, bacteria dominated Sediminibacterium fungi Mycosphaerella Alternaria Cladosporium . Redundancy analysis confirmed that trichomes facilitated capture -associated fungi, less impacted struggled adhere microstructures. Long provided ideal microhabitats retaining PM-borne microbes, feedback loops characteristics, relative abundances microorganisms like Trichoderma Aspergillus Conclusions Based our findings, three-factor network profile was constructed, provides foundation further exploration into how different plants thereby impacting
Язык: Английский
Процитировано
8
Journal of Fungi, Год журнала: 2025, Номер 11(1), С. 77 - 77
Опубликована: Янв. 18, 2025
This review delves into innovative technologies to improve the control of vascular fungal plant pathogens. It also briefly summarizes traditional biocontrol approaches manage them, addressing their limitations and emphasizing need develop more sustainable precise solutions. Powerful tools such as next-generation sequencing, meta-omics, microbiome engineering allow for targeted manipulation microbial communities enhance pathogen suppression. Microbiome-based include design synthetic consortia transplant entire or customized soil/plant microbiomes, potentially offering resilient adaptable strategies. Nanotechnology has advanced significantly, providing methods delivery biological agents (BCAs) compounds derived from them through different nanoparticles (NPs), including bacteriogenic, mycogenic, phytogenic, phycogenic, debris-derived ones acting carriers. The use biodegradable polymeric non-polymeric eco-friendly NPs, which enable controlled release antifungal while minimizing environmental impact, is explored. Furthermore, artificial intelligence machine learning can revolutionize crop protection early disease detection, prediction outbreaks, precision in BCA treatments. Other genome editing, RNA interference (RNAi), functional peptides efficacy against pathogenic fungi. Altogether, these provide a comprehensive framework management diseases, redefining modern agriculture.
Язык: Английский
Процитировано
1
Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(1)
Опубликована: Янв. 14, 2025
The use of biological control agents is one the best strategies available to combat plant diseases in an ecofriendly manner. Biocontrol bacteria capable providing beneficial effect crop growth and health, have been developed for several decades. It highlights need a deeper understanding colonization mechanisms employed by biocontrol enhance their efficacy pathogen control. present review deals with in-depth steps involved host bacteria. process starts from root zone, where establish initial interactions plant’s system. Moving beyond roots, migrate colonize other organs, including stems, leaves, even flowers. Also, attempts explore facilitating bacterial movement within such as migrating through interconnected spaces vessels or apoplast, applying quorum sensing extracellular enzymes during what needed long-term association plant. impacts on microbial community dynamics, nutrient cycling, overall health are discussed, emphasizing intricate relationships between microbiome benefits above-ground parts, 40 confer. By unraveling these mechanisms, researchers can develop targeted enhancing efficiency effectiveness bacteria, leading more sustainability resilience.
Язык: Английский
Процитировано
1
Journal of Food Science, Год журнала: 2025, Номер 90(2)
Опубликована: Фев. 1, 2025
Abstract The oxidative species generated by cold atmospheric plasma (CAP) treatment can impact the plant stress response system. We hypothesized that this is not limited to site of CAP application and it transmitted through plant. resulting influence microbiome on intact These hypotheses were tested live sweet basil ( Ocimum basilicum var. Kiera). A single upper leaf underwent a 60 s at three different wattage intensity levels. Reactive oxygen (ROS) generation in directly treated leaves vicinity (i.e., one, two, or nodes away) was measured using fluorescein degradation assay (ex/em: 485/525). Leaves exposed showed marked increase ROS production. Interestingly, also significant p < 0.05) compared untreated control, extending two nearest from all plants tested. microbiomes evaluated 16S rRNA gene sequencing. appeared drive restructuring microbiota profiles, despite maintaining similar α‐diversity. led differences relative abundances variety dominant bacterial families (e.g., Psuedomonadaceae Streptomycetaceae) phyla, effects certain taxa dependent distance site. CAP's ability restructure may have applications improve produce microbial safety shelf‐life. Practical Application Cold induces living beyond application. This includes an production reactive trigger pathways enhance phytochemicals. alters community profile, possibly response. Results study be useful developing for improved growth, health‐benefiting phytochemicals, managing its microbiota.
Язык: Английский
Процитировано
1
The Science of The Total Environment, Год журнала: 2024, Номер 929, С. 172622 - 172622
Опубликована: Апрель 19, 2024
Язык: Английский
Процитировано
5
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(46)
Опубликована: Ноя. 4, 2024
To address rising global food demand, the development of sustainable technologies to increase productivity is urgently needed. This study revealed that foliar application zinc oxide nanoparticles (ZnO NPs; 30 80 nm, 0.67 mg/d per plant, 6 d) rice leaves under heatwave (HW) stress increased grain yield and nutritional quality. Compared with HW control, HWs+ZnO group presented increases in yield, protein content, amino acid content 22.1%, 11.8%, 77.5%, respectively. Nanoscale ZnO aggregated on leaf surface interacted molecules. at ambient temperature, treatment dissolution NPs by 25.9% facilitated their translocation mesophyll cells. The Zn existed as both ionic particulate ZnO. conditions nutrient levels (Zn, Mn, Cu, Fe, Mg) 15.8 416.9%, chlorophyll 22.2 24.8%, Rubisco enzyme activity 21.2%, antioxidant 26.7 31.2%. Transcriptomic analyses reversed HW-induced transcriptomic dysregulation, thereby enhancing photosynthesis 74.4%. Additionally, diversity, stability, enrichment beneficial microbial taxa protected phyllosphere community from damage. work elucidates how interact phyllosphere, highlighting potential promote agriculture, especially extreme climate events (e.g., HWs).
Язык: Английский
Процитировано
5
Biomolecules, Год журнала: 2025, Номер 15(2), С. 252 - 252
Опубликована: Фев. 9, 2025
Plant microbiomes play a major role in plant health, growth, and development, enhancing resistance to pathogen invasion. However, despite the extensive research on phyllosphere microbiome, it remains unclear how microbiome of leaves proximity diseased responds We investigate response maize white spot by assessing dynamics associated with portion area using 16S ITS high-throughput sequencing analysis. Our results showed that bacterial diversities were higher than those healthy plants. At same time, lower fungal diversity was recorded compared portions leaves. The had significant influence microbial composition. portion, it, dominated genera Sphingomonas, Delftia, Chryseobacterium, Stenotrophomonas, Methylobacterium-methylorubrum, Bacteroides. Still, abundance Sphingomonas decreased corresponding increase Stenotrophomonas. Conversely, genus Setophoma while pathogens Cladosporium, Alternaria, Exserohilum highly abundant samples from it. In addition, co-occurrence network analysis revealed complex infected portion. This study suggests leaf disease is colonized more harmful pathogenic fungi for progression.
Язык: Английский
Процитировано
0
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Апрель 1, 2025
Phyllosphere-associated microbes directly influence plant-pathogen interactions, and the external environment plant shape phyllosphere microbiome. In this study, we integrated 16S rRNA ITS high-throughput sequencing to systematically investigate changes in microbiome between symptomatic asymptomatic walnut leaves affected by spot disease, with consideration of phenological stage progression. Additionally, explored how abiotic (AT, DT, SCTCC & LPDD) biotic factors (Pn Gs) impact microbial communities. Our findings revealed significant differences diversity at same stage. Furthermore, structure function phyllosphere-associated changed as progressed. Fungal taxa that related Plant_Pathogen bacterial KEGG pathway functions Fatty acid biosynthesis Biotin metabolism were increased group. The keystone species driving was Pseudomonas spp., which substantially influenced vs. leaves. Notably, spp. interacted Xanthomonas Pantoea Correlation analysis dew point temperature constituted primary factor community composition, whereas liquid precipitation depth dimension identified dominant shaping fungal taxa. leaf net photosynthetic rate stomatal conductance closely linked These results advance our understanding community-level responses pathogen invasion highlight multifactorial drivers assembly. Ultimately, they contribute predicting managing leaf-related diseases.
Язык: Английский
Процитировано
0
Microbiological Research, Год журнала: 2025, Номер 297, С. 128184 - 128184
Опубликована: Апрель 14, 2025
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Процитировано
0