Biomass for sustainable air quality

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 123 - 137

Опубликована: Янв. 1, 2025

Язык: Английский

Semirational Design and Immobilization Synergistically Enhance Barnase Activity and Stability

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Barnase, derived from Bacillus amyloliquefaciens, is a key enzyme in biocatalysis with widespread applications pharmaceutical synthesis. However, its stability under extreme conditions, such as high temperatures and pH, limits industrial applications. Therefore, enhancing both catalytic efficiency through genetic engineering has become critical focus of research. In this study, AlphaFold was employed to predict the structure followed by molecular docking dynamics simulations using GROMACS design construct 24 mutants. The results demonstrated that enzymatic activity S28H D101 K mutants increased 75.28% 71.86%, respectively, while declined temperatures. To address this, immobilized onto ZIF-8 carrier. Under optimized immobilization conditions (1.5 M 2-methylimidazole, 1.5 mL solution, 20 °C), ZIF-8@D101 exhibited significantly enhanced thermal pH adaptability. Recycling experiments showed 96.21% retained after three cycles, 72.47% eight demonstrating superior reusability stability, making it more suitable for

Язык: Английский

Catalytic conversion of woody biomass into monomeric chemicals by a microwave mediated metal-organic-framework 818

Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119864 - 119864

Опубликована: Окт. 19, 2024

Язык: Английский