
Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 119864 - 119864
Published: Oct. 19, 2024
Language: Английский
Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 119864 - 119864
Published: Oct. 19, 2024
Language: Английский
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 123 - 137
Published: Jan. 1, 2025
Language: Английский
Citations
0Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 14, 2025
Barnase, derived from Bacillus amyloliquefaciens, is a key enzyme in biocatalysis with widespread applications pharmaceutical synthesis. However, its stability under extreme conditions, such as high temperatures and pH, limits industrial applications. Therefore, enhancing both catalytic efficiency through genetic engineering has become critical focus of research. In this study, AlphaFold was employed to predict the structure followed by molecular docking dynamics simulations using GROMACS design construct 24 mutants. The results demonstrated that enzymatic activity S28H D101 K mutants increased 75.28% 71.86%, respectively, while declined temperatures. To address this, immobilized onto ZIF-8 carrier. Under optimized immobilization conditions (1.5 M 2-methylimidazole, 1.5 mL solution, 20 °C), ZIF-8@D101 exhibited significantly enhanced thermal pH adaptability. Recycling experiments showed 96.21% retained after three cycles, 72.47% eight demonstrating superior reusability stability, making it more suitable for
Language: Английский
Citations
0Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 119864 - 119864
Published: Oct. 19, 2024
Language: Английский
Citations
1