Advancements in genetic engineering for enhanced Polyhydroxyalkanoates (PHA) production: a comprehensive review of metabolic pathway manipulation and gene deletion strategies

Bioengineered, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 30, 2025

Polyhydroxyalkanoates (PHA) are bioplastics produced by few bacteria as intracellular lipid inclusions under excess carbon source and nutrient-deprived conditions. These polymers biodegradable resemble petroleum-based plastics. The rising environmental concerns have increased the demand for PHA, but low yield in wild-type bacterial strains limits large-scale production. An improvement PHA production can be achieved genetically engineering removing competitive pathways that divert metabolites away from biosynthesis, cloning strong promotors to overexpress genes involved biosynthesis constructing non-native metabolic feed desired monomers were obtained elimination of biosynthetic pathway. chain length degradation specific-gene deletion β-oxidation pathway resulted accumulation having high length. A controlled polymer was novel deleting native genome non-PHA producers. present review attempts showcase genetic modification approaches conducted so far enhance with a special focus on gene various bacteria.

Язык: Английский

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Alternatives to Conventional Plastics: Polyhydroxyalkanoates (PHA) from Microbial Sources and Recent Approaches – A Review

Process Safety and Environmental Protection, Год журнала: 2025, Номер unknown, С. 106809 - 106809

Опубликована: Янв. 1, 2025

Язык: Английский

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Recent Trends in the Production and Recovery of Bioplastics Using Polyhydroxyalkanoates Copolymers

Microorganisms, Год журнала: 2024, Номер 12(11), С. 2135 - 2135

Опубликована: Окт. 24, 2024

Polyhydroxyalkanoates (PHAs) are polyesters synthesized as a carbon and energy reserve material by wide number of bacteria. These polymers characterized their thermoplastic properties similar to those plastics derived from the petrochemical industry, such polyethylene polypropylene. PHAs widely used in medical field have potential be other applications due biocompatibility biodegradability. Among PHAs, P(3HB-

Язык: Английский

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Research Hot Spots and Development Trends of Biodegradable Plastics

Process Biochemistry, Год журнала: 2024, Номер 150, С. 80 - 93

Опубликована: Дек. 23, 2024

Язык: Английский

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