A positive feedback loop controls Toxoplasma chronic differentiation

Nature Microbiology, Год журнала: 2023, Номер 8(5), С. 889 - 904

Опубликована: Апрель 20, 2023

Язык: Английский

---

Zinc Finger Proteins in Neuro-Related Diseases Progression

Frontiers in Neuroscience, Год журнала: 2021, Номер 15

Опубликована: Ноя. 18, 2021

Zinc finger proteins (ZNF) are among the most abundant in eukaryotic genomes. It contains several zinc domains that can selectively bind to certain DNA or RNA and associate with proteins, therefore, ZNF regulate gene expression at transcriptional translational levels. In terms of neurological diseases, numerous studies have shown many associated diseases. The purpose this review is summarize types roles neuropsychiatric disorders. We will describe structure classification ZNF, then focus on pathophysiological role neuro-related diseases mechanism action

Язык: Английский

---

Natural products modulate NLRP3 in ulcerative colitis

Frontiers in Pharmacology, Год журнала: 2023, Номер 14

Опубликована: Окт. 2, 2023

Ulcerative colitis (UC) is a clinically common, progressive, devastating, chronic inflammatory disease of the intestine that recurrent and difficult to treat. Nod-like receptor protein 3 (NLRP3) complex composed multiple proteins whose formation activates cysteine aspartate protease-1 (caspase-1) induce maturation secretion mediators such as interleukin (IL)-1β IL-18, promoting development responses. Recent studies have shown NLRP3 associated with UC susceptibility, it maintains stable intestinal environment by responding wide range pathogenic microorganisms. The mainstay treatment for control inflammation relieve symptoms. Despite certain curative effect, there are problems easy recurrence after drug withdrawal many side effects long-term medication. serves core link in response. If relationship between gut microbes inflammation-associated factors can be analyzed concerning its related signaling pathways, expression status well specific mechanism course IBD elucidated further considered clinical diagnosis IBD, expected lead compounds targeting inflammasome developed IBD. Research into prevention UC, which has become hotbed research recent years, natural products rich therapeutic means, multi-targets, fewer adverse effects. Natural promise treating numerous basic trials over past few years. This paper describes regulatory role against provides reference this disease.

Язык: Английский

---

MKRN1 promotes colorectal cancer metastasis by activating the TGF-β signalling pathway through SNIP1 protein degradation

Journal of Experimental & Clinical Cancer Research, Год журнала: 2023, Номер 42(1)

Опубликована: Авг. 24, 2023

The Makorin ring finger protein 1 (MKRN1) gene, also called RNF61, is located on the long arm of chromosome 7 and a member RING family. E3 ubiquitin ligase MKRN1 closely linked to tumour development, but exact mechanism needs be elucidated. In this study, we aimed investigate specific role in colorectal cancer (CRC) development.MKRN1 expression CRC was analysed using Cancer Cell Line Encyclopaedia Genome Atlas (TCGA) databases. Rectal tissues were frozen explore its clinical significance. impact cell proliferation migration observed CCK8, colony formation, wound healing, transwell assays. A combination quantitative proteomics, ubiquitination modification omics analysis, string vitro vivo experiments revealed potential mechanisms by which regulates metastasis.MKRN1 significantly elevated compared paracancerous positively with prognosis (P < 0.01). downregulation inhibits proliferation, migration, invasion. Conversely, overexpression promotes invasion cells. Mechanistically, induces epithelial-mesenchymal transition (EMT) cells via degradation Smad nuclear-interacting (SNIP1). Furthermore, SNIP1 transforming growth factor-β (TGF-β) signalling, TGF-β signalling degrading induce EMT Finally, conditional knockout mice, intestinal lesions metastatic liver microlesions greatly reduced group that control group.High levels promote through SNIP1, thereby facilitating metastasis, supporting as pro-cancer factor. MKRN1/SNIP1/TGF-β axis may therapeutic target CRC.

Язык: Английский

---

Zinc finger proteins of Plasmodium falciparum

Cellular Microbiology, Год журнала: 2021, Номер 23(12)

Опубликована: Авг. 21, 2021

Zinc finger proteins (ZFPs) are a large diverse family of with one or more zinc domains in which is important stabilising the domain. ZFPs can interact DNA, RNA, lipids even other and therefore contribute to cellular processes including transcriptional regulation, ubiquitin-mediated protein degradation, mRNA decay stability. In this review, we provide first comprehensive classification malaria parasite Plasmodium falciparum state knowledge on main parasite, include C2H2, CCCH, RING PHD proteins. TAKE AWAYS: The genome encodes 170 putative (ZFPs). subfamilies most represented. Known ZFP functions regulation metabolism proteostasis.

Язык: Английский

---

The role of ubiquitination and deubiquitination in PI3K/AKT/mTOR pathway: A potential target for cancer therapy

Gene, Год журнала: 2023, Номер 889, С. 147807 - 147807

Опубликована: Сен. 16, 2023

Язык: Английский

---

Identification and catalogue of viral transcriptional regulators in human diseases

iScience, Год журнала: 2025, Номер 28(3), С. 112081 - 112081

Опубликована: Фев. 21, 2025

Язык: Английский

---

Molecular Functions and Potential Utilization of Zinc Finger Proteins in Protozoan Parasites

Опубликована: Апрель 10, 2025

Zinc finger proteins (ZFP) are metalloproteins whose zinc atom interacts with side chains of specific histidines and cysteines the motif, generating functional three dimensional structures. A good number these from different species similar while others divergent in structure function. Existing literature on structural features, functions their potential to be targeted for drug development or used as molecular tools parasitic protozoa was explored. Evidence reviewed articles revealed that, just like other eukaryotes, function various parasite cellular processes such as: transcription, RNA editing, mRNA processing & export and, turn-over among others. Some them had same features homologues eukaryotes including hosts. However, there were which studies aiming at replacing reported positive results some parasites Giardia lamblia, Trichomonas vaginalis Leishmania donovani though this is promising, off- target challenge that can cause effects, must solved before wide application achieved. Successful engineering use genome editing also Plasmodium falciparum. Since nucleases a powerful research medical tool, repurposing ZFPs useful therapy discovery. For succeed, more knowledge how regulated needed.

Язык: Английский

---

The Plasmodium falciparum CCCH Zinc Finger Protein ZNF4 Plays an Important Role in Gametocyte Exflagellation through the Regulation of Male Enriched Transcripts

Cells, Год журнала: 2022, Номер 11(10), С. 1666 - 1666

Опубликована: Май 17, 2022

CCCH zinc finger proteins (ZFPs) function mainly as RNA-binding (RBPs) and play a central role in the mRNA metabolism. Over twenty seven CCCH-ZFPs are encoded genome of human malaria parasite Plasmodium falciparum, causative agent tropica. However, little is known about their functions. In this study, we characterize one member PfCCCH-ZFP named ZNF4. We show that ZNF4 highly expressed mature gametocytes, where it predominantly localizes to cytoplasm. Targeted gene disruption showed no significant effect asexual blood stage replication gametocyte development while male exflagellation was significantly impaired, leading reduced transmission mosquito. Comparative transcriptomics between wildtype (WT) ZNF4-deficient line (ZNF4-KO) demonstrated deregulation 473 genes (274 upregulated 199 downregulated) gametocytes. Most downregulated peak expression with enriched associated axonemal dynein complex formation, cell projection organization affected, pointing phenotype exflagellation. Upregulated ATP synthesis. Our combined data therefore indicate protein which plays an important through regulation gametocyte-enriched genes.

Язык: Английский

---

The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts

Journal of Biological Chemistry, Год журнала: 2023, Номер 299(8), С. 104959 - 104959

Опубликована: Июнь 24, 2023

Nuclear mRNA metabolism is regulated by multiple proteins, which either directly bind to RNA or form multiprotein complexes. The RNA-binding protein ZC3H11A involved in nuclear export, NF-κB signaling, and essential during mouse embryo development. Furthermore, previous studies have shown that important for nuclear-replicating viruses. However, detailed biochemical characterization of the has been lacking. In this study, we established interactome human cells. We demonstrate poly(A)-binding PABPN1 interacts specifically with controls localization into speckles. report adenovirus type 5 (HAdV-5) capsid a PABPN1-dependent manner. Notably, uses same zinc finger motifs interact viral mRNA. Further, lack alters polyadenylation HAdV-5 Taken together, our results suggest may act as novel regulator

Язык: Английский

---